Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles

Superparamagnetic iron oxide (SPIO) nanoparticles, either modified or in combination with other macromolecules, are being used for magnetic labeling of stem cells and other cells to monitor cell trafficking by magnetic resonance imaging (MRI) in experimental models. The correlation of histology to M...

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Main Authors: Joseph A. Frank, Heather Kalish, E. Kay Jordan, Stasia A. Anderson, Edyta Pawelczyk, Ali S. Arbab
Format: Article
Language:English
Published: Hindawi - SAGE Publishing 2007-05-01
Series:Molecular Imaging
Online Access:https://doi.org/10.2310/7290.2007.00014
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spelling doaj-972fbf0884a742d19f6664406d3abc332021-04-02T12:37:50ZengHindawi - SAGE PublishingMolecular Imaging1536-01212007-05-01610.2310/7290.2007.0001410.2310_7290.2007.00014Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide NanoparticlesJoseph A. FrankHeather KalishE. Kay JordanStasia A. AndersonEdyta PawelczykAli S. ArbabSuperparamagnetic iron oxide (SPIO) nanoparticles, either modified or in combination with other macromolecules, are being used for magnetic labeling of stem cells and other cells to monitor cell trafficking by magnetic resonance imaging (MRI) in experimental models. The correlation of histology to MRI depends on the ability to detect SPIO-labeled cells using Prussian blue (PB) stain and fluorescent tags to cell surface markers. Exposure of PB-positive sections to ultraviolet light at a wavelength of 365 nm commonly used fluorescence microscopy can result in color transformation of PB-positive material from blue to brown. Although the PB color transformation is primarily an artifact that may occur during fluorescence microscopy, the transformation can be manipulated using imaging process software for the detection of low levels of iron labeled cells in tissues sampleshttps://doi.org/10.2310/7290.2007.00014
collection DOAJ
language English
format Article
sources DOAJ
author Joseph A. Frank
Heather Kalish
E. Kay Jordan
Stasia A. Anderson
Edyta Pawelczyk
Ali S. Arbab
spellingShingle Joseph A. Frank
Heather Kalish
E. Kay Jordan
Stasia A. Anderson
Edyta Pawelczyk
Ali S. Arbab
Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles
Molecular Imaging
author_facet Joseph A. Frank
Heather Kalish
E. Kay Jordan
Stasia A. Anderson
Edyta Pawelczyk
Ali S. Arbab
author_sort Joseph A. Frank
title Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles
title_short Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles
title_full Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles
title_fullStr Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles
title_full_unstemmed Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles
title_sort color transformation and fluorescence of prussian blue–positive cells: implications for histologic verification of cells labeled with superparamagnetic iron oxide nanoparticles
publisher Hindawi - SAGE Publishing
series Molecular Imaging
issn 1536-0121
publishDate 2007-05-01
description Superparamagnetic iron oxide (SPIO) nanoparticles, either modified or in combination with other macromolecules, are being used for magnetic labeling of stem cells and other cells to monitor cell trafficking by magnetic resonance imaging (MRI) in experimental models. The correlation of histology to MRI depends on the ability to detect SPIO-labeled cells using Prussian blue (PB) stain and fluorescent tags to cell surface markers. Exposure of PB-positive sections to ultraviolet light at a wavelength of 365 nm commonly used fluorescence microscopy can result in color transformation of PB-positive material from blue to brown. Although the PB color transformation is primarily an artifact that may occur during fluorescence microscopy, the transformation can be manipulated using imaging process software for the detection of low levels of iron labeled cells in tissues samples
url https://doi.org/10.2310/7290.2007.00014
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