Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water
Jaguar (Panthera onca) are of conservation concern and occur at very low densities in the northern portion of their range in northern Mexico and the southwestern United States. Environmental DNA sampling to detect genetic material from drinking water may be an effective approach for jaguar detection...
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doaj-973beca9b6944ee8904f1c556500f4182021-05-13T04:24:19ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2021-05-01910.3389/fevo.2021.613200613200Detection of Jaguar (Panthera onca) From Genetic Material in Drinking WaterTaylor M. Wilcox0Anthony Caragiulo1Joseph C. Dysthe2Thomas W. Franklin3Daniel H. Mason4Kevin S. McKelvey5Katherine E. Zarn6Michael K. Schwartz7National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, USDA Forest Service, Missoula, MT, United StatesSackler Institute for Comparative Genomics, American Museum of Natural History, New York, NY, United StatesNational Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, USDA Forest Service, Missoula, MT, United StatesNational Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, USDA Forest Service, Missoula, MT, United StatesNational Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, USDA Forest Service, Missoula, MT, United StatesNational Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, USDA Forest Service, Missoula, MT, United StatesNational Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, USDA Forest Service, Missoula, MT, United StatesNational Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, USDA Forest Service, Missoula, MT, United StatesJaguar (Panthera onca) are of conservation concern and occur at very low densities in the northern portion of their range in northern Mexico and the southwestern United States. Environmental DNA sampling to detect genetic material from drinking water may be an effective approach for jaguar detection in these arid landscapes. Here we develop a qPCR assay for the detection of jaguar mitochondrial DNA, show that large quantities of DNA (mean 66,820 copies/L) can be found in the drinking water of captive animals, and observe detectable levels of DNA (80 copies/L) in a wild habitat with known jaguar populations. We suggest that environmental DNA sampling may represent a useful, complementary sampling tool for detection of rare jaguars, although effective application would require careful consideration of DNA persistence time in the environment.https://www.frontiersin.org/articles/10.3389/fevo.2021.613200/fullenvironmental DNAeDNAnon-invasive geneticswildlifemonitoring |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Taylor M. Wilcox Anthony Caragiulo Joseph C. Dysthe Thomas W. Franklin Daniel H. Mason Kevin S. McKelvey Katherine E. Zarn Michael K. Schwartz |
spellingShingle |
Taylor M. Wilcox Anthony Caragiulo Joseph C. Dysthe Thomas W. Franklin Daniel H. Mason Kevin S. McKelvey Katherine E. Zarn Michael K. Schwartz Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water Frontiers in Ecology and Evolution environmental DNA eDNA non-invasive genetics wildlife monitoring |
author_facet |
Taylor M. Wilcox Anthony Caragiulo Joseph C. Dysthe Thomas W. Franklin Daniel H. Mason Kevin S. McKelvey Katherine E. Zarn Michael K. Schwartz |
author_sort |
Taylor M. Wilcox |
title |
Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water |
title_short |
Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water |
title_full |
Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water |
title_fullStr |
Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water |
title_full_unstemmed |
Detection of Jaguar (Panthera onca) From Genetic Material in Drinking Water |
title_sort |
detection of jaguar (panthera onca) from genetic material in drinking water |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Ecology and Evolution |
issn |
2296-701X |
publishDate |
2021-05-01 |
description |
Jaguar (Panthera onca) are of conservation concern and occur at very low densities in the northern portion of their range in northern Mexico and the southwestern United States. Environmental DNA sampling to detect genetic material from drinking water may be an effective approach for jaguar detection in these arid landscapes. Here we develop a qPCR assay for the detection of jaguar mitochondrial DNA, show that large quantities of DNA (mean 66,820 copies/L) can be found in the drinking water of captive animals, and observe detectable levels of DNA (80 copies/L) in a wild habitat with known jaguar populations. We suggest that environmental DNA sampling may represent a useful, complementary sampling tool for detection of rare jaguars, although effective application would require careful consideration of DNA persistence time in the environment. |
topic |
environmental DNA eDNA non-invasive genetics wildlife monitoring |
url |
https://www.frontiersin.org/articles/10.3389/fevo.2021.613200/full |
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