Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins.
Respiratory syncytial virus (RSV) is a single-stranded RNA virus that assembles into viral filaments at the cell surface. Virus assembly often depends on the ability of a virus to use host proteins to accomplish viral tasks. Since the fusion protein cytoplasmic tail (FCT) is critical for viral filam...
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doaj-974d60faea5648759a990536d9eda9912020-11-25T01:56:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4082610.1371/journal.pone.0040826Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins.Fyza Y ShaikhThomas J UtleyRyan E CravenMeredith C RogersLynne A LapierreJames R GoldenringJames E CroweRespiratory syncytial virus (RSV) is a single-stranded RNA virus that assembles into viral filaments at the cell surface. Virus assembly often depends on the ability of a virus to use host proteins to accomplish viral tasks. Since the fusion protein cytoplasmic tail (FCT) is critical for viral filamentous assembly, we hypothesized that host proteins important for viral assembly may be recruited by the FCT. Using a yeast two-hybrid screen, we found that filamin A interacted with FCT, and mammalian cell experiments showed it localized to viral filaments but did not affect viral replication. Furthermore, we found that a number of actin-associated proteins also were excluded from viral filaments. Actin or tubulin cytoskeletal rearrangement was not necessary for F trafficking to the cell surface or for viral assembly into filaments, but was necessary for optimal viral replication and may be important for anchoring viral filaments. These findings suggest that RSV assembly into filaments occurs independently of actin polymerization and that viral proteins are the principal drivers for the mechanical tasks involved with formation of complex, structured RSV filaments at the host cell plasma membrane.http://europepmc.org/articles/PMC3396619?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fyza Y Shaikh Thomas J Utley Ryan E Craven Meredith C Rogers Lynne A Lapierre James R Goldenring James E Crowe |
spellingShingle |
Fyza Y Shaikh Thomas J Utley Ryan E Craven Meredith C Rogers Lynne A Lapierre James R Goldenring James E Crowe Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins. PLoS ONE |
author_facet |
Fyza Y Shaikh Thomas J Utley Ryan E Craven Meredith C Rogers Lynne A Lapierre James R Goldenring James E Crowe |
author_sort |
Fyza Y Shaikh |
title |
Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins. |
title_short |
Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins. |
title_full |
Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins. |
title_fullStr |
Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins. |
title_full_unstemmed |
Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins. |
title_sort |
respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Respiratory syncytial virus (RSV) is a single-stranded RNA virus that assembles into viral filaments at the cell surface. Virus assembly often depends on the ability of a virus to use host proteins to accomplish viral tasks. Since the fusion protein cytoplasmic tail (FCT) is critical for viral filamentous assembly, we hypothesized that host proteins important for viral assembly may be recruited by the FCT. Using a yeast two-hybrid screen, we found that filamin A interacted with FCT, and mammalian cell experiments showed it localized to viral filaments but did not affect viral replication. Furthermore, we found that a number of actin-associated proteins also were excluded from viral filaments. Actin or tubulin cytoskeletal rearrangement was not necessary for F trafficking to the cell surface or for viral assembly into filaments, but was necessary for optimal viral replication and may be important for anchoring viral filaments. These findings suggest that RSV assembly into filaments occurs independently of actin polymerization and that viral proteins are the principal drivers for the mechanical tasks involved with formation of complex, structured RSV filaments at the host cell plasma membrane. |
url |
http://europepmc.org/articles/PMC3396619?pdf=render |
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