Rifampin resistance among individuals with extrapulmonary tuberculosis: 4 years of experience from a reference laboratory
Information is limited about the drug resistance patterns in extrapulmonary tuberculosis (EPTB) in Iran. This study aimed to determine the prevalence of EPTB and to investigate the drug-resistance pattern in Mycobacterium tuberculosis strains collected from extrapulmonary samples at the Tehran regio...
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doaj-974eb26b32a34d5d89476c91d5dfce6c2021-03-25T04:28:15ZengElsevierNew Microbes and New Infections2052-29752021-03-0140100841Rifampin resistance among individuals with extrapulmonary tuberculosis: 4 years of experience from a reference laboratoryS. Baghbanbashi0S. Mohammad J. Mousavi1H. Dabiri2M. Hakemi-Vala3H. Goudarzi4G. Hamzehloo5S. Amini6M.J. Nasiri7Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranRegional Tuberculosis Reference Laboratory, Tehran University of Medical Sciences, Tehran, IranRegional Tuberculosis Reference Laboratory, Tehran University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Corresponding author: M.J. Nasiri, Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Information is limited about the drug resistance patterns in extrapulmonary tuberculosis (EPTB) in Iran. This study aimed to determine the prevalence of EPTB and to investigate the drug-resistance pattern in Mycobacterium tuberculosis strains collected from extrapulmonary samples at the Tehran regional TB reference laboratory. Extrapulmonary specimens from individuals with suspected TB referred to the TB reference laboratories in five cities of Iran were collected. Both standard conventional methods (culture and direct smear microscopy) and Xpert MTB/RIF assay were used for the identification of mycobacteria. Drug susceptibility testing was done using Xpert MTB/RIF. The proportion method on Lowenstein–Jensen medium was performed for confirmation. Between 2016 and 2020, a total of 12 050 clinical specimens from individuals with suspected TB were collected, of which 10 380 (86%) were pulmonary specimens and 1670 (14%) were extrapulmonary. Of the extrapulmonary specimens, 85 (5.0%) were positive for M. tuberculosis, and the remaining 1585 (95.0%) samples were negative by standard methods. Of 85 M. tuberculosis isolates, drug susceptibility testing was performed for 32 isolates, of which 1 (3.1%, 95% CI 0.0%–9.4%) was rifampin resistant and 31 (96.9%, 95% CI 90.1%–100%) were pan-susceptible. The rifampin-resistant isolate was also resistant to isoniazid, so was assigned as a multidrug-resistant TB. Our study indicated the frequency of drug-resistance among EPTB in Iran. Establishing rapid diagnostic methods for detection of drug-resistance in EPTB, performing drug susceptibility testing for all EPTB cases to provide effective treatment, and continuous monitoring of drug resistance, are suggested for prevention and control of drug resistance in EPTB in Iran.http://www.sciencedirect.com/science/article/pii/S2052297521000056Drug resistanceextrapulmonary tuberculosisIranMycobacterium tuberculosisrifampin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
S. Baghbanbashi S. Mohammad J. Mousavi H. Dabiri M. Hakemi-Vala H. Goudarzi G. Hamzehloo S. Amini M.J. Nasiri |
spellingShingle |
S. Baghbanbashi S. Mohammad J. Mousavi H. Dabiri M. Hakemi-Vala H. Goudarzi G. Hamzehloo S. Amini M.J. Nasiri Rifampin resistance among individuals with extrapulmonary tuberculosis: 4 years of experience from a reference laboratory New Microbes and New Infections Drug resistance extrapulmonary tuberculosis Iran Mycobacterium tuberculosis rifampin |
author_facet |
S. Baghbanbashi S. Mohammad J. Mousavi H. Dabiri M. Hakemi-Vala H. Goudarzi G. Hamzehloo S. Amini M.J. Nasiri |
author_sort |
S. Baghbanbashi |
title |
Rifampin resistance among individuals with extrapulmonary tuberculosis: 4 years of experience from a reference laboratory |
title_short |
Rifampin resistance among individuals with extrapulmonary tuberculosis: 4 years of experience from a reference laboratory |
title_full |
Rifampin resistance among individuals with extrapulmonary tuberculosis: 4 years of experience from a reference laboratory |
title_fullStr |
Rifampin resistance among individuals with extrapulmonary tuberculosis: 4 years of experience from a reference laboratory |
title_full_unstemmed |
Rifampin resistance among individuals with extrapulmonary tuberculosis: 4 years of experience from a reference laboratory |
title_sort |
rifampin resistance among individuals with extrapulmonary tuberculosis: 4 years of experience from a reference laboratory |
publisher |
Elsevier |
series |
New Microbes and New Infections |
issn |
2052-2975 |
publishDate |
2021-03-01 |
description |
Information is limited about the drug resistance patterns in extrapulmonary tuberculosis (EPTB) in Iran. This study aimed to determine the prevalence of EPTB and to investigate the drug-resistance pattern in Mycobacterium tuberculosis strains collected from extrapulmonary samples at the Tehran regional TB reference laboratory. Extrapulmonary specimens from individuals with suspected TB referred to the TB reference laboratories in five cities of Iran were collected. Both standard conventional methods (culture and direct smear microscopy) and Xpert MTB/RIF assay were used for the identification of mycobacteria. Drug susceptibility testing was done using Xpert MTB/RIF. The proportion method on Lowenstein–Jensen medium was performed for confirmation. Between 2016 and 2020, a total of 12 050 clinical specimens from individuals with suspected TB were collected, of which 10 380 (86%) were pulmonary specimens and 1670 (14%) were extrapulmonary. Of the extrapulmonary specimens, 85 (5.0%) were positive for M. tuberculosis, and the remaining 1585 (95.0%) samples were negative by standard methods. Of 85 M. tuberculosis isolates, drug susceptibility testing was performed for 32 isolates, of which 1 (3.1%, 95% CI 0.0%–9.4%) was rifampin resistant and 31 (96.9%, 95% CI 90.1%–100%) were pan-susceptible. The rifampin-resistant isolate was also resistant to isoniazid, so was assigned as a multidrug-resistant TB. Our study indicated the frequency of drug-resistance among EPTB in Iran. Establishing rapid diagnostic methods for detection of drug-resistance in EPTB, performing drug susceptibility testing for all EPTB cases to provide effective treatment, and continuous monitoring of drug resistance, are suggested for prevention and control of drug resistance in EPTB in Iran. |
topic |
Drug resistance extrapulmonary tuberculosis Iran Mycobacterium tuberculosis rifampin |
url |
http://www.sciencedirect.com/science/article/pii/S2052297521000056 |
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