Protein malnutrition during gestation and early life decreases neuronal size in the medial prefrontal cortex of post-pubertal rats
Retrospective studies in human populations indicate that protein deprivation during pregnancy and early life (early protein malnutrition, EPM) is associated with cognitive impairments, learning disabilities and may represent a risk factor for the late onset of some psychiatric disorders, fundamental...
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doaj-97526675826c45c78db862f6c17838672020-11-24T23:58:41ZengElsevierIBRO Reports2451-83012017-12-013C657110.1016/j.ibror.2017.08.002Protein malnutrition during gestation and early life decreases neuronal size in the medial prefrontal cortex of post-pubertal ratsRoelf J. Cruz-Rizzolo0Laís Leal Limieri1Isabela Rogério de Paiva2Jéssica O. Barbosa Ribeiro3Taís Fernandes Pimenta4Luciana Pinato5Edilson Ervolino6Cláudio Aparecido Casatti7Leila M. Guissoni Campos8Edson Aparecido Liberti9Department of Basic Sciences, São Paulo State University, Araçatuba, SP, BrazilDepartment of Basic Sciences, São Paulo State University, Araçatuba, SP, BrazilDepartment of Basic Sciences, São Paulo State University, Araçatuba, SP, BrazilDepartment of Basic Sciences, São Paulo State University, Araçatuba, SP, BrazilDepartment of Basic Sciences, São Paulo State University, Araçatuba, SP, BrazilDepartment of Speech-Language and Hearing Therapy, São Paulo State University, Marilia, SP, BrazilDepartment of Basic Sciences, São Paulo State University, Araçatuba, SP, BrazilDepartment of Basic Sciences, São Paulo State University, Araçatuba, SP, BrazilDepartment of Anatomy, School of Medicine, University of Marilia, SP, BrazilDepartment of Anatomy, Institute of Biomedical Sciences, University of São Paulo, SP, BrazilRetrospective studies in human populations indicate that protein deprivation during pregnancy and early life (early protein malnutrition, EPM) is associated with cognitive impairments, learning disabilities and may represent a risk factor for the late onset of some psychiatric disorders, fundamentally schizophrenia, a condition where the prefrontal cortex plays an important role. The purpose of this study was to analyze whether EPM affects structural aspects of the rat medial prefrontal cortex (mPFC), such as cortical volume, neuronal density and neuronal soma size, which seem altered in patients with schizophrenia. For this, a rat model of EPM (5% casein from conception to postnatal day 60) was adopted and the rat mPFC volume, total number of neurons and average neuronal volume were evaluated on postnatal day 60 (post-pubertal animals) by histo- and immunohistochemical techniques using unbiased stereological analysis. EPM did not alter the number of NeuN+ neurons in the rat mPFC. However, a very significant decrease in mPFC volume and average neuronal size was observed in malnourished rats. Although the present study does not establish causal relationships between malnutrition and schizophrenia, our results may indicate a similar structural phenomenon in these two situations.http://www.sciencedirect.com/science/article/pii/S2451830117300213MalnutritionPrefrontal cortexNeuronal sizeNeuronal densityNeuNStereologySchizophrenia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roelf J. Cruz-Rizzolo Laís Leal Limieri Isabela Rogério de Paiva Jéssica O. Barbosa Ribeiro Taís Fernandes Pimenta Luciana Pinato Edilson Ervolino Cláudio Aparecido Casatti Leila M. Guissoni Campos Edson Aparecido Liberti |
spellingShingle |
Roelf J. Cruz-Rizzolo Laís Leal Limieri Isabela Rogério de Paiva Jéssica O. Barbosa Ribeiro Taís Fernandes Pimenta Luciana Pinato Edilson Ervolino Cláudio Aparecido Casatti Leila M. Guissoni Campos Edson Aparecido Liberti Protein malnutrition during gestation and early life decreases neuronal size in the medial prefrontal cortex of post-pubertal rats IBRO Reports Malnutrition Prefrontal cortex Neuronal size Neuronal density NeuN Stereology Schizophrenia |
author_facet |
Roelf J. Cruz-Rizzolo Laís Leal Limieri Isabela Rogério de Paiva Jéssica O. Barbosa Ribeiro Taís Fernandes Pimenta Luciana Pinato Edilson Ervolino Cláudio Aparecido Casatti Leila M. Guissoni Campos Edson Aparecido Liberti |
author_sort |
Roelf J. Cruz-Rizzolo |
title |
Protein malnutrition during gestation and early life decreases neuronal size in the medial prefrontal cortex of post-pubertal rats |
title_short |
Protein malnutrition during gestation and early life decreases neuronal size in the medial prefrontal cortex of post-pubertal rats |
title_full |
Protein malnutrition during gestation and early life decreases neuronal size in the medial prefrontal cortex of post-pubertal rats |
title_fullStr |
Protein malnutrition during gestation and early life decreases neuronal size in the medial prefrontal cortex of post-pubertal rats |
title_full_unstemmed |
Protein malnutrition during gestation and early life decreases neuronal size in the medial prefrontal cortex of post-pubertal rats |
title_sort |
protein malnutrition during gestation and early life decreases neuronal size in the medial prefrontal cortex of post-pubertal rats |
publisher |
Elsevier |
series |
IBRO Reports |
issn |
2451-8301 |
publishDate |
2017-12-01 |
description |
Retrospective studies in human populations indicate that protein deprivation during pregnancy and early life (early protein malnutrition, EPM) is associated with cognitive impairments, learning disabilities and may represent a risk factor for the late onset of some psychiatric disorders, fundamentally schizophrenia, a condition where the prefrontal cortex plays an important role. The purpose of this study was to analyze whether EPM affects structural aspects of the rat medial prefrontal cortex (mPFC), such as cortical volume, neuronal density and neuronal soma size, which seem altered in patients with schizophrenia. For this, a rat model of EPM (5% casein from conception to postnatal day 60) was adopted and the rat mPFC volume, total number of neurons and average neuronal volume were evaluated on postnatal day 60 (post-pubertal animals) by histo- and immunohistochemical techniques using unbiased stereological analysis. EPM did not alter the number of NeuN+ neurons in the rat mPFC. However, a very significant decrease in mPFC volume and average neuronal size was observed in malnourished rats. Although the present study does not establish causal relationships between malnutrition and schizophrenia, our results may indicate a similar structural phenomenon in these two situations. |
topic |
Malnutrition Prefrontal cortex Neuronal size Neuronal density NeuN Stereology Schizophrenia |
url |
http://www.sciencedirect.com/science/article/pii/S2451830117300213 |
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