Formulation development of itraconazole PEGylated nano-lipid carriers for pulmonary aspergillosis using hot-melt extrusion technology
Pulmonary delivery is a promising alternative for the oral treatment of pulmonary aspergillosis. This study aimed to develop continuous and scalable itraconazole PEGylated nano-lipid carriers (ITZ-PEG-NLC) for inhalation delivery. The feasibility of preparing NLCs utilizing hot-melt extrusion (HME)...
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doaj-976cb0ee04e84701b828b8aaae3ada872021-03-15T04:26:50ZengElsevierInternational Journal of Pharmaceutics: X2590-15672021-12-013100074Formulation development of itraconazole PEGylated nano-lipid carriers for pulmonary aspergillosis using hot-melt extrusion technologyGauri Shadambikar0Sushrut Marathe1Nan Ji2Mashan Almutairi3Suresh Bandari4Feng Zhang5Mahavir Chougule6Michael Repka7Department of Pharmaceutics and Drug Delivery, University of Mississippi, University, MS 38677, USADepartment of Pharmaceutics and Drug Delivery, University of Mississippi, University, MS 38677, USADepartment of Pharmaceutics and Drug Delivery, University of Mississippi, University, MS 38677, USADepartment of Pharmaceutics and Drug Delivery, University of Mississippi, University, MS 38677, USA; Department of Pharmaceutics, College of Pharmacy, University of Hail, Hail 81442, Saudi ArabiaDepartment of Pharmaceutics and Drug Delivery, University of Mississippi, University, MS 38677, USACollege of Pharmacy, The University of Texas at Austin, TX 78712, USADepartment of Pharmaceutics and Drug Delivery, University of Mississippi, University, MS 38677, USADepartment of Pharmaceutics and Drug Delivery, University of Mississippi, University, MS 38677, USA; Pii Center for Pharmaceutical Technology, University of Mississippi, University, MS 38677, USA; Corresponding author at: Department of Pharmaceutics and Drug Delivery, Pii Center for Pharmaceutical Technology, School of Pharmacy, University of Mississippi, University, MS 38677, USA.Pulmonary delivery is a promising alternative for the oral treatment of pulmonary aspergillosis. This study aimed to develop continuous and scalable itraconazole PEGylated nano-lipid carriers (ITZ-PEG-NLC) for inhalation delivery. The feasibility of preparing NLCs utilizing hot-melt extrusion (HME) coupled with probe sonication was investigated. The process parameters for HME and sonication were varied to optimize the formulation. ITZ-PEG-NLC (particle size, 101.20 ± 1.69 nm; polydispersity index, 0.26 ± 0.01) was successfully formulated. The drug entrapment efficiency of ITZ-PEG-NLC was 97.28 ± 0.50%. Transmission electron microscopy was used to characterize the shape of the particles. The developed formulations were evaluated for their aerodynamic properties for pulmonary delivery. The lung deposition of ITZ-PEG-NLC was determined using an Anderson Cascade Impactor and Philips Respironics Sami the Seal Nebulizer Compressor. In vitro cytotoxicity studies were performed using A549 cells. A burst-release pattern was observed in ITZ-PEG-NLC with a drug release of 41.74 ± 1.49% in 60 min. The in vitro aerosolization of the ITZ-PEG-NLC formulation showed a mass median aerodynamic diameter of 3.51 ± 0.28 μm and a geometric standard deviation of 2.44 ± 0.49. These findings indicate that HME technology could be used for the production of continuous scalable ITZ-PEG-NLC.http://www.sciencedirect.com/science/article/pii/S2590156721000037ItraconazolePEGylationHot-melt extrusionPulmonary drug deliveryInhalationNanostructured lipid carriers |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gauri Shadambikar Sushrut Marathe Nan Ji Mashan Almutairi Suresh Bandari Feng Zhang Mahavir Chougule Michael Repka |
spellingShingle |
Gauri Shadambikar Sushrut Marathe Nan Ji Mashan Almutairi Suresh Bandari Feng Zhang Mahavir Chougule Michael Repka Formulation development of itraconazole PEGylated nano-lipid carriers for pulmonary aspergillosis using hot-melt extrusion technology International Journal of Pharmaceutics: X Itraconazole PEGylation Hot-melt extrusion Pulmonary drug delivery Inhalation Nanostructured lipid carriers |
author_facet |
Gauri Shadambikar Sushrut Marathe Nan Ji Mashan Almutairi Suresh Bandari Feng Zhang Mahavir Chougule Michael Repka |
author_sort |
Gauri Shadambikar |
title |
Formulation development of itraconazole PEGylated nano-lipid carriers for pulmonary aspergillosis using hot-melt extrusion technology |
title_short |
Formulation development of itraconazole PEGylated nano-lipid carriers for pulmonary aspergillosis using hot-melt extrusion technology |
title_full |
Formulation development of itraconazole PEGylated nano-lipid carriers for pulmonary aspergillosis using hot-melt extrusion technology |
title_fullStr |
Formulation development of itraconazole PEGylated nano-lipid carriers for pulmonary aspergillosis using hot-melt extrusion technology |
title_full_unstemmed |
Formulation development of itraconazole PEGylated nano-lipid carriers for pulmonary aspergillosis using hot-melt extrusion technology |
title_sort |
formulation development of itraconazole pegylated nano-lipid carriers for pulmonary aspergillosis using hot-melt extrusion technology |
publisher |
Elsevier |
series |
International Journal of Pharmaceutics: X |
issn |
2590-1567 |
publishDate |
2021-12-01 |
description |
Pulmonary delivery is a promising alternative for the oral treatment of pulmonary aspergillosis. This study aimed to develop continuous and scalable itraconazole PEGylated nano-lipid carriers (ITZ-PEG-NLC) for inhalation delivery. The feasibility of preparing NLCs utilizing hot-melt extrusion (HME) coupled with probe sonication was investigated. The process parameters for HME and sonication were varied to optimize the formulation. ITZ-PEG-NLC (particle size, 101.20 ± 1.69 nm; polydispersity index, 0.26 ± 0.01) was successfully formulated. The drug entrapment efficiency of ITZ-PEG-NLC was 97.28 ± 0.50%. Transmission electron microscopy was used to characterize the shape of the particles. The developed formulations were evaluated for their aerodynamic properties for pulmonary delivery. The lung deposition of ITZ-PEG-NLC was determined using an Anderson Cascade Impactor and Philips Respironics Sami the Seal Nebulizer Compressor. In vitro cytotoxicity studies were performed using A549 cells. A burst-release pattern was observed in ITZ-PEG-NLC with a drug release of 41.74 ± 1.49% in 60 min. The in vitro aerosolization of the ITZ-PEG-NLC formulation showed a mass median aerodynamic diameter of 3.51 ± 0.28 μm and a geometric standard deviation of 2.44 ± 0.49. These findings indicate that HME technology could be used for the production of continuous scalable ITZ-PEG-NLC. |
topic |
Itraconazole PEGylation Hot-melt extrusion Pulmonary drug delivery Inhalation Nanostructured lipid carriers |
url |
http://www.sciencedirect.com/science/article/pii/S2590156721000037 |
work_keys_str_mv |
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