In Vitro Anthelmintic Activity of Four Plant-Derived Compounds against Sheep Gastrointestinal Nematodes
By using the egg hatch test (EHT), the larval development test (LDT) and the larval mortality/paralysis test (LMT), the in vitro anthelmintic activity on sheep gastrointestinal strongyles (GIS) of four plant-derived pure compounds, mangiferin (at 0.25%, 0.125% and 0.0625%), rutin (at 1%, 0.75%, 0.5%...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-09-01
|
Series: | Veterinary Sciences |
Subjects: | |
Online Access: | http://www.mdpi.com/2306-7381/5/3/78 |
id |
doaj-9780ff3de26d4f35b56e21db204e3021 |
---|---|
record_format |
Article |
spelling |
doaj-9780ff3de26d4f35b56e21db204e30212021-04-02T03:15:27ZengMDPI AGVeterinary Sciences2306-73812018-09-01537810.3390/vetsci5030078vetsci5030078In Vitro Anthelmintic Activity of Four Plant-Derived Compounds against Sheep Gastrointestinal NematodesFederica Giovanelli0Matteo Mattellini1Gianluca Fichi2Guido Flamini3Stefania Perrucci4Dipartimento di Scienze Veterinarie, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDipartimento di Scienze Veterinarie, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDipartimento di Scienze Veterinarie, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDipartimento di Farmacia, University of Pisa, Via Bonanno Pisano 6, 56126 Pisa, ItalyDipartimento di Scienze Veterinarie, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyBy using the egg hatch test (EHT), the larval development test (LDT) and the larval mortality/paralysis test (LMT), the in vitro anthelmintic activity on sheep gastrointestinal strongyles (GIS) of four plant-derived pure compounds, mangiferin (at 0.25%, 0.125% and 0.0625%), rutin (at 1%, 0.75%, 0.5%), quercetin (at 1%), and β-sitosterol (at 1%, 0.75%, 0.5%), was investigated. For comparison, untreated and treated (0.1% thiabendazole, 0.1% TBZ) controls were used. Six repetitions were made throughout the experiment. Data were statistically elaborated using the χ2 test. The concentration able to inhibit the development of the 50% of L1s to L3s and causing the mortality of the 50% of L3s (EC50) was also calculated. L3s recovered from untreated Petri dishes were identified at the genus level. In EHT, all tested compounds at all concentrations significantly (p < 0.01) inhibited the hatch of the eggs when compared to the untreated controls, but none of them was as effective as 0.1% TBZ. In LDT, rutin (at 1%, 0.75% and 0.5%), mangiferin (at 0.25% and 0.125%), β-sitosterol (at 1%) and 0.1% TBZ completely prevented the larval development from L1 to L3 in respect to the untreated controls (p < 0.01). In LMT, all tested compounds significantly (p < 0.01) increased the death of L3s compared to the untreated controls, except for β-sitosterol at 0.5%. However, only rutin at all concentrations and 0.25% and 0.125% mangiferin were as effective as 0.1% TBZ. Haemonchus, Trichostrongylus, Chabertia and Teladorsagia/Ostertagia GIS genera, were identified.http://www.mdpi.com/2306-7381/5/3/78mangiferinrutinquercetinβ-sitosterolanthelmintic activityin vitrogastrointestinal strongylessheep |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Federica Giovanelli Matteo Mattellini Gianluca Fichi Guido Flamini Stefania Perrucci |
spellingShingle |
Federica Giovanelli Matteo Mattellini Gianluca Fichi Guido Flamini Stefania Perrucci In Vitro Anthelmintic Activity of Four Plant-Derived Compounds against Sheep Gastrointestinal Nematodes Veterinary Sciences mangiferin rutin quercetin β-sitosterol anthelmintic activity in vitro gastrointestinal strongyles sheep |
author_facet |
Federica Giovanelli Matteo Mattellini Gianluca Fichi Guido Flamini Stefania Perrucci |
author_sort |
Federica Giovanelli |
title |
In Vitro Anthelmintic Activity of Four Plant-Derived Compounds against Sheep Gastrointestinal Nematodes |
title_short |
In Vitro Anthelmintic Activity of Four Plant-Derived Compounds against Sheep Gastrointestinal Nematodes |
title_full |
In Vitro Anthelmintic Activity of Four Plant-Derived Compounds against Sheep Gastrointestinal Nematodes |
title_fullStr |
In Vitro Anthelmintic Activity of Four Plant-Derived Compounds against Sheep Gastrointestinal Nematodes |
title_full_unstemmed |
In Vitro Anthelmintic Activity of Four Plant-Derived Compounds against Sheep Gastrointestinal Nematodes |
title_sort |
in vitro anthelmintic activity of four plant-derived compounds against sheep gastrointestinal nematodes |
publisher |
MDPI AG |
series |
Veterinary Sciences |
issn |
2306-7381 |
publishDate |
2018-09-01 |
description |
By using the egg hatch test (EHT), the larval development test (LDT) and the larval mortality/paralysis test (LMT), the in vitro anthelmintic activity on sheep gastrointestinal strongyles (GIS) of four plant-derived pure compounds, mangiferin (at 0.25%, 0.125% and 0.0625%), rutin (at 1%, 0.75%, 0.5%), quercetin (at 1%), and β-sitosterol (at 1%, 0.75%, 0.5%), was investigated. For comparison, untreated and treated (0.1% thiabendazole, 0.1% TBZ) controls were used. Six repetitions were made throughout the experiment. Data were statistically elaborated using the χ2 test. The concentration able to inhibit the development of the 50% of L1s to L3s and causing the mortality of the 50% of L3s (EC50) was also calculated. L3s recovered from untreated Petri dishes were identified at the genus level. In EHT, all tested compounds at all concentrations significantly (p < 0.01) inhibited the hatch of the eggs when compared to the untreated controls, but none of them was as effective as 0.1% TBZ. In LDT, rutin (at 1%, 0.75% and 0.5%), mangiferin (at 0.25% and 0.125%), β-sitosterol (at 1%) and 0.1% TBZ completely prevented the larval development from L1 to L3 in respect to the untreated controls (p < 0.01). In LMT, all tested compounds significantly (p < 0.01) increased the death of L3s compared to the untreated controls, except for β-sitosterol at 0.5%. However, only rutin at all concentrations and 0.25% and 0.125% mangiferin were as effective as 0.1% TBZ. Haemonchus, Trichostrongylus, Chabertia and Teladorsagia/Ostertagia GIS genera, were identified. |
topic |
mangiferin rutin quercetin β-sitosterol anthelmintic activity in vitro gastrointestinal strongyles sheep |
url |
http://www.mdpi.com/2306-7381/5/3/78 |
work_keys_str_mv |
AT federicagiovanelli invitroanthelminticactivityoffourplantderivedcompoundsagainstsheepgastrointestinalnematodes AT matteomattellini invitroanthelminticactivityoffourplantderivedcompoundsagainstsheepgastrointestinalnematodes AT gianlucafichi invitroanthelminticactivityoffourplantderivedcompoundsagainstsheepgastrointestinalnematodes AT guidoflamini invitroanthelminticactivityoffourplantderivedcompoundsagainstsheepgastrointestinalnematodes AT stefaniaperrucci invitroanthelminticactivityoffourplantderivedcompoundsagainstsheepgastrointestinalnematodes |
_version_ |
1724173925319966720 |