Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China

Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China

Virulence genes of Escherichia coli (E. coli) isolates from healthy dairy cows were identified and characterized by a multiplex PCR assay and serogrouping test. The results showed that among the target genes, eaeA was most frequently detected, accounting for 22.11% (67/303) in all strains from 101 c...

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Main Authors: Simujide Huasai, Aorigele Chen, Chun-jie Wang, Yu Li, Bai Tongrige
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia 2012-06-01
Series:Brazilian Journal of Microbiology
Subjects:
E. coli
Virulence gene
Multiplex PCR
Serogroup
Healthy dairy cow
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200013
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spelling doaj-9785ee52597849438555bb216355a25a2020-11-24T21:36:00ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1517-83821678-44052012-06-0143252853410.1590/S1517-83822012000200013Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, ChinaSimujide HuasaiAorigele ChenChun-jie WangYu LiBai TongrigeVirulence genes of Escherichia coli (E. coli) isolates from healthy dairy cows were identified and characterized by a multiplex PCR assay and serogrouping test. The results showed that among the target genes, eaeA was most frequently detected, accounting for 22.11% (67/303) in all strains from 101 cows. For categorization of E. coli, aEPEC was the category with widest distribution detected in 55 (18.15%) strains from 22 cattle. All of 84 PCR-positive strains belonged to 14 O serogroups, and O149 (25.00%) was most common identified, followed by O2 (17.86%), O8 (11.90%) and O103 (9.52%) with relatively high prevalence.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200013E. coliVirulence geneMultiplex PCRSerogroupHealthy dairy cow
collection DOAJ
language English
format Article
sources DOAJ
author Simujide Huasai
Aorigele Chen
Chun-jie Wang
Yu Li
Bai Tongrige
spellingShingle Simujide Huasai
Aorigele Chen
Chun-jie Wang
Yu Li
Bai Tongrige
Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China
Brazilian Journal of Microbiology
E. coli
Virulence gene
Multiplex PCR
Serogroup
Healthy dairy cow
author_facet Simujide Huasai
Aorigele Chen
Chun-jie Wang
Yu Li
Bai Tongrige
author_sort Simujide Huasai
title Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China
title_short Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China
title_full Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China
title_fullStr Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China
title_full_unstemmed Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China
title_sort occurrence and characteristics of virulence genes of escherichia coli strains isolated from healthy dairy cows in inner mongolia, china
publisher Sociedade Brasileira de Microbiologia
series Brazilian Journal of Microbiology
issn 1517-8382
1678-4405
publishDate 2012-06-01
description Virulence genes of Escherichia coli (E. coli) isolates from healthy dairy cows were identified and characterized by a multiplex PCR assay and serogrouping test. The results showed that among the target genes, eaeA was most frequently detected, accounting for 22.11% (67/303) in all strains from 101 cows. For categorization of E. coli, aEPEC was the category with widest distribution detected in 55 (18.15%) strains from 22 cattle. All of 84 PCR-positive strains belonged to 14 O serogroups, and O149 (25.00%) was most common identified, followed by O2 (17.86%), O8 (11.90%) and O103 (9.52%) with relatively high prevalence.
topic E. coli
Virulence gene
Multiplex PCR
Serogroup
Healthy dairy cow
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200013
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