Visualization and identification of IL-7 producing cells in reporter mice.
Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide an...
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2009-11-01
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doaj-97899add48644baaaae8623b3d0db2532020-11-25T00:02:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-11-01411e763710.1371/journal.pone.0007637Visualization and identification of IL-7 producing cells in reporter mice.Renata I MazzucchelliSøren WarmingScott M LawrenceMasaru IshiiMehrnoosh AbshariA Valance WashingtonLionel FeigenbaumAndrew C WarnerDavis J SimsWen Qing LiJulie A HixonDaniel H D GrayBenjamin E RichMatthew MorrowMiriam R AnverJames CherryDieter NafLawrence R SternbergDaniel W McVicarAndrew G FarrRonald N GermainKeith RogersNancy A JenkinsNeal G CopelandScott K DurumInterleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP (enhanced cyan fluorescent protein) accumulated inside IL-7-producing stromal cells in thoracic thymus, cervical thymus and bone marrow. In thymus, an extensive reticular network of IL-7-containing processes extended from cortical and medullary epithelial cells, closely contacting thymocytes. Central memory CD8 T cells, which require IL-7 and home to bone marrow, physically associated with IL-7-producing cells as we demonstrate by intravital imaging.http://europepmc.org/articles/PMC2770321?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Renata I Mazzucchelli Søren Warming Scott M Lawrence Masaru Ishii Mehrnoosh Abshari A Valance Washington Lionel Feigenbaum Andrew C Warner Davis J Sims Wen Qing Li Julie A Hixon Daniel H D Gray Benjamin E Rich Matthew Morrow Miriam R Anver James Cherry Dieter Naf Lawrence R Sternberg Daniel W McVicar Andrew G Farr Ronald N Germain Keith Rogers Nancy A Jenkins Neal G Copeland Scott K Durum |
spellingShingle |
Renata I Mazzucchelli Søren Warming Scott M Lawrence Masaru Ishii Mehrnoosh Abshari A Valance Washington Lionel Feigenbaum Andrew C Warner Davis J Sims Wen Qing Li Julie A Hixon Daniel H D Gray Benjamin E Rich Matthew Morrow Miriam R Anver James Cherry Dieter Naf Lawrence R Sternberg Daniel W McVicar Andrew G Farr Ronald N Germain Keith Rogers Nancy A Jenkins Neal G Copeland Scott K Durum Visualization and identification of IL-7 producing cells in reporter mice. PLoS ONE |
author_facet |
Renata I Mazzucchelli Søren Warming Scott M Lawrence Masaru Ishii Mehrnoosh Abshari A Valance Washington Lionel Feigenbaum Andrew C Warner Davis J Sims Wen Qing Li Julie A Hixon Daniel H D Gray Benjamin E Rich Matthew Morrow Miriam R Anver James Cherry Dieter Naf Lawrence R Sternberg Daniel W McVicar Andrew G Farr Ronald N Germain Keith Rogers Nancy A Jenkins Neal G Copeland Scott K Durum |
author_sort |
Renata I Mazzucchelli |
title |
Visualization and identification of IL-7 producing cells in reporter mice. |
title_short |
Visualization and identification of IL-7 producing cells in reporter mice. |
title_full |
Visualization and identification of IL-7 producing cells in reporter mice. |
title_fullStr |
Visualization and identification of IL-7 producing cells in reporter mice. |
title_full_unstemmed |
Visualization and identification of IL-7 producing cells in reporter mice. |
title_sort |
visualization and identification of il-7 producing cells in reporter mice. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2009-11-01 |
description |
Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP (enhanced cyan fluorescent protein) accumulated inside IL-7-producing stromal cells in thoracic thymus, cervical thymus and bone marrow. In thymus, an extensive reticular network of IL-7-containing processes extended from cortical and medullary epithelial cells, closely contacting thymocytes. Central memory CD8 T cells, which require IL-7 and home to bone marrow, physically associated with IL-7-producing cells as we demonstrate by intravital imaging. |
url |
http://europepmc.org/articles/PMC2770321?pdf=render |
work_keys_str_mv |
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