Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) against the virulence of uropathogenic Escherichia coli
Abstract Uropathogenic Escherichia coli (UPEC), the most prevalent bacteria isolated in urinary tract infections (UTI), is now frequently resistant to antibiotics used to treat this pathology. The antibacterial properties of cranberry and propolis could reduce the frequency of UTIs and thus the use...
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2018-07-01
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Online Access: | https://doi.org/10.1038/s41598-018-29082-6 |
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doaj-9789d38ed42245a0b13c98d57ede97022020-12-08T06:21:05ZengNature Publishing GroupScientific Reports2045-23222018-07-018111110.1038/s41598-018-29082-6Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) against the virulence of uropathogenic Escherichia coliJérémy Ranfaing0Catherine Dunyach-Remy1Laurence Louis2Jean-Philippe Lavigne3Albert Sotto4French National Institute of Health and Medical Research, Unit 1047, University Montpellier, Faculty of MedicineFrench National Institute of Health and Medical Research, Unit 1047, University Montpellier, Faculty of MedicinePlateforme Génomique et transcriptomique, UMR_S 910, University Aix-Marseille, Faculty of Medicine La TimoneFrench National Institute of Health and Medical Research, Unit 1047, University Montpellier, Faculty of MedicineFrench National Institute of Health and Medical Research, Unit 1047, University Montpellier, Faculty of MedicineAbstract Uropathogenic Escherichia coli (UPEC), the most prevalent bacteria isolated in urinary tract infections (UTI), is now frequently resistant to antibiotics used to treat this pathology. The antibacterial properties of cranberry and propolis could reduce the frequency of UTIs and thus the use of antibiotics, helping in the fight against the emergence of antibiotic resistance. Transcriptomic profiles of a clinical UPEC strain exposed to cranberry proanthocyanidins alone (190 µg/mL), propolis alone (102.4 µg/mL) and a combination of both were determined. Cranberry alone, but more so cranberry + propolis combined, modified the expression of genes involved in different essential pathways: down-expression of genes involved in adhesion, motility, and biofilm formation, and up-regulation of genes involved in iron metabolism and stress response. Phenotypic assays confirmed the decrease of motility (swarming and swimming) and biofilm formation (early formation and formed biofilm). This study showed for the first time that propolis potentiated the effect of cranberry proanthocyanidins on adhesion, motility, biofilm formation, iron metabolism and stress response of UPEC. Cranberry + propolis treatment could represent an interesting new strategy to prevent recurrent UTI.https://doi.org/10.1038/s41598-018-29082-6 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jérémy Ranfaing Catherine Dunyach-Remy Laurence Louis Jean-Philippe Lavigne Albert Sotto |
spellingShingle |
Jérémy Ranfaing Catherine Dunyach-Remy Laurence Louis Jean-Philippe Lavigne Albert Sotto Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) against the virulence of uropathogenic Escherichia coli Scientific Reports |
author_facet |
Jérémy Ranfaing Catherine Dunyach-Remy Laurence Louis Jean-Philippe Lavigne Albert Sotto |
author_sort |
Jérémy Ranfaing |
title |
Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) against the virulence of uropathogenic Escherichia coli |
title_short |
Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) against the virulence of uropathogenic Escherichia coli |
title_full |
Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) against the virulence of uropathogenic Escherichia coli |
title_fullStr |
Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) against the virulence of uropathogenic Escherichia coli |
title_full_unstemmed |
Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) against the virulence of uropathogenic Escherichia coli |
title_sort |
propolis potentiates the effect of cranberry (vaccinium macrocarpon) against the virulence of uropathogenic escherichia coli |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-07-01 |
description |
Abstract Uropathogenic Escherichia coli (UPEC), the most prevalent bacteria isolated in urinary tract infections (UTI), is now frequently resistant to antibiotics used to treat this pathology. The antibacterial properties of cranberry and propolis could reduce the frequency of UTIs and thus the use of antibiotics, helping in the fight against the emergence of antibiotic resistance. Transcriptomic profiles of a clinical UPEC strain exposed to cranberry proanthocyanidins alone (190 µg/mL), propolis alone (102.4 µg/mL) and a combination of both were determined. Cranberry alone, but more so cranberry + propolis combined, modified the expression of genes involved in different essential pathways: down-expression of genes involved in adhesion, motility, and biofilm formation, and up-regulation of genes involved in iron metabolism and stress response. Phenotypic assays confirmed the decrease of motility (swarming and swimming) and biofilm formation (early formation and formed biofilm). This study showed for the first time that propolis potentiated the effect of cranberry proanthocyanidins on adhesion, motility, biofilm formation, iron metabolism and stress response of UPEC. Cranberry + propolis treatment could represent an interesting new strategy to prevent recurrent UTI. |
url |
https://doi.org/10.1038/s41598-018-29082-6 |
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