Contrasting Effects of Wild Arachis Dehydrin Under Abiotic and Biotic Stresses
Plant dehydrins (DNHs) belong to the LEA (Late Embryogenesis Abundant) protein family and are involved in responses to multiple abiotic stresses. DHNs are classified into five subclasses according to the organization of three conserved motifs (K-; Y-; and S-segments). In the present study, the DHN p...
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doaj-978f3d1144294b5da6689655573a5cdd2020-11-24T21:52:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-04-011010.3389/fpls.2019.00497443681Contrasting Effects of Wild Arachis Dehydrin Under Abiotic and Biotic StressesAna Paula Zotta Mota0Ana Paula Zotta Mota1Thais Nicolini Oliveira2Thais Nicolini Oliveira3Christina Cleo Vinson4Christina Cleo Vinson5Thomas Christopher Rhys Williams6Marcos Mota do Carmo Costa7Ana Claudia Guerra Araujo8Etienne G. J. Danchin9Maria Fatima Grossi-de-Sá10Patricia Messenberg Guimaraes11Ana Cristina Miranda Brasileiro12EMBRAPA Recursos Genéticos e Biotecnologia, Brasília, BrazilDepartamento de Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul, Porto Alegre, BrazilEMBRAPA Recursos Genéticos e Biotecnologia, Brasília, BrazilDepartamento de Botânica, Universidade de Brasília, Brasília, BrazilEMBRAPA Recursos Genéticos e Biotecnologia, Brasília, BrazilDepartamento de Botânica, Universidade de Brasília, Brasília, BrazilDepartamento de Botânica, Universidade de Brasília, Brasília, BrazilEMBRAPA Recursos Genéticos e Biotecnologia, Brasília, BrazilEMBRAPA Recursos Genéticos e Biotecnologia, Brasília, BrazilINRA, Université Côte d’Azur, CNRS, ISA, Sophia-Antipolis, FranceEMBRAPA Recursos Genéticos e Biotecnologia, Brasília, BrazilEMBRAPA Recursos Genéticos e Biotecnologia, Brasília, BrazilEMBRAPA Recursos Genéticos e Biotecnologia, Brasília, BrazilPlant dehydrins (DNHs) belong to the LEA (Late Embryogenesis Abundant) protein family and are involved in responses to multiple abiotic stresses. DHNs are classified into five subclasses according to the organization of three conserved motifs (K-; Y-; and S-segments). In the present study, the DHN protein family was characterized by molecular phylogeny, exon/intron organization, protein structure, and tissue-specificity expression in eight Fabaceae species. We identified 20 DHN genes, encompassing three (YnSKn, SKn, and Kn) subclasses sharing similar gene organization and protein structure. Two additional low conserved DHN Φ-segments specific to the legume SKn-type of proteins were also found. The in silico expression patterns of DHN genes in four legume species (Arachis duranensis, A. ipaënsis, Glycine max, and Medicago truncatula) revealed that their tissue-specific regulation is associated with the presence or absence of the Y-segment. Indeed, DHN genes containing a Y-segment are mainly expressed in seeds, whereas those without the Y-segment are ubiquitously expressed. Further qRT-PCR analysis revealed that, amongst stress responsive dehydrins, a SKn-type DHN gene from A. duranensis (AdDHN1) showed opposite response to biotic and abiotic stress with a positive regulation under water deficit and negative regulation upon nematode infection. Furthermore, transgenic Arabidopsis lines overexpressing (OE) AdDHN1 displayed improved tolerance to multiple abiotic stresses (freezing and drought) but increased susceptibility to the biotrophic root-knot nematode (RKN) Meloidogyne incognita. This contradictory role of AdDHN1 in responses to abiotic and biotic stresses was further investigated by qRT-PCR analysis of transgenic plants using a set of stress-responsive genes involved in the abscisic acid (ABA) and jasmonic acid (JA) signaling pathways and suggested an involvement of DHN overexpression in these stress-signaling pathways.https://www.frontiersin.org/article/10.3389/fpls.2019.00497/fullArabidopsisdroughtfreezinggenome-wideMeloidogyneroot-knot nematode |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana Paula Zotta Mota Ana Paula Zotta Mota Thais Nicolini Oliveira Thais Nicolini Oliveira Christina Cleo Vinson Christina Cleo Vinson Thomas Christopher Rhys Williams Marcos Mota do Carmo Costa Ana Claudia Guerra Araujo Etienne G. J. Danchin Maria Fatima Grossi-de-Sá Patricia Messenberg Guimaraes Ana Cristina Miranda Brasileiro |
spellingShingle |
Ana Paula Zotta Mota Ana Paula Zotta Mota Thais Nicolini Oliveira Thais Nicolini Oliveira Christina Cleo Vinson Christina Cleo Vinson Thomas Christopher Rhys Williams Marcos Mota do Carmo Costa Ana Claudia Guerra Araujo Etienne G. J. Danchin Maria Fatima Grossi-de-Sá Patricia Messenberg Guimaraes Ana Cristina Miranda Brasileiro Contrasting Effects of Wild Arachis Dehydrin Under Abiotic and Biotic Stresses Frontiers in Plant Science Arabidopsis drought freezing genome-wide Meloidogyne root-knot nematode |
author_facet |
Ana Paula Zotta Mota Ana Paula Zotta Mota Thais Nicolini Oliveira Thais Nicolini Oliveira Christina Cleo Vinson Christina Cleo Vinson Thomas Christopher Rhys Williams Marcos Mota do Carmo Costa Ana Claudia Guerra Araujo Etienne G. J. Danchin Maria Fatima Grossi-de-Sá Patricia Messenberg Guimaraes Ana Cristina Miranda Brasileiro |
author_sort |
Ana Paula Zotta Mota |
title |
Contrasting Effects of Wild Arachis Dehydrin Under Abiotic and Biotic Stresses |
title_short |
Contrasting Effects of Wild Arachis Dehydrin Under Abiotic and Biotic Stresses |
title_full |
Contrasting Effects of Wild Arachis Dehydrin Under Abiotic and Biotic Stresses |
title_fullStr |
Contrasting Effects of Wild Arachis Dehydrin Under Abiotic and Biotic Stresses |
title_full_unstemmed |
Contrasting Effects of Wild Arachis Dehydrin Under Abiotic and Biotic Stresses |
title_sort |
contrasting effects of wild arachis dehydrin under abiotic and biotic stresses |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2019-04-01 |
description |
Plant dehydrins (DNHs) belong to the LEA (Late Embryogenesis Abundant) protein family and are involved in responses to multiple abiotic stresses. DHNs are classified into five subclasses according to the organization of three conserved motifs (K-; Y-; and S-segments). In the present study, the DHN protein family was characterized by molecular phylogeny, exon/intron organization, protein structure, and tissue-specificity expression in eight Fabaceae species. We identified 20 DHN genes, encompassing three (YnSKn, SKn, and Kn) subclasses sharing similar gene organization and protein structure. Two additional low conserved DHN Φ-segments specific to the legume SKn-type of proteins were also found. The in silico expression patterns of DHN genes in four legume species (Arachis duranensis, A. ipaënsis, Glycine max, and Medicago truncatula) revealed that their tissue-specific regulation is associated with the presence or absence of the Y-segment. Indeed, DHN genes containing a Y-segment are mainly expressed in seeds, whereas those without the Y-segment are ubiquitously expressed. Further qRT-PCR analysis revealed that, amongst stress responsive dehydrins, a SKn-type DHN gene from A. duranensis (AdDHN1) showed opposite response to biotic and abiotic stress with a positive regulation under water deficit and negative regulation upon nematode infection. Furthermore, transgenic Arabidopsis lines overexpressing (OE) AdDHN1 displayed improved tolerance to multiple abiotic stresses (freezing and drought) but increased susceptibility to the biotrophic root-knot nematode (RKN) Meloidogyne incognita. This contradictory role of AdDHN1 in responses to abiotic and biotic stresses was further investigated by qRT-PCR analysis of transgenic plants using a set of stress-responsive genes involved in the abscisic acid (ABA) and jasmonic acid (JA) signaling pathways and suggested an involvement of DHN overexpression in these stress-signaling pathways. |
topic |
Arabidopsis drought freezing genome-wide Meloidogyne root-knot nematode |
url |
https://www.frontiersin.org/article/10.3389/fpls.2019.00497/full |
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