Development of diagnostic tools for IBDV detection using plants as bioreactors
Abstract Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 h...
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doaj-978fe17b8a1d4d1a9648769e10c19c812020-11-25T03:33:17ZengSpringerOpenAMB Express2191-08552020-05-011011910.1186/s13568-020-01029-zDevelopment of diagnostic tools for IBDV detection using plants as bioreactorsEvangelina Gómez0María Florencia Cassani1María Soledad Lucero2Viviana Parreño3Silvina Chimeno Zoth4Analía Berinstein5Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)INCUINTA & Instituto de Virología e Innovaciones Tecnológicas (IVIT), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Abstract Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana benthamiana. The SVP, which were observed by electronic microscopy, proved to be antigenically and immunogenically similar to the virion. Indeed, anti-IBDV antibodies from samples of infected birds recognized these SVP and, when injected intramuscularly, these subviral particles also evoked a humoral immune response in chickens. We developed an in-house ELISA using SVP as coating reagent that demonstrated to be highly accurate and in good agreement with a commercial ELISA. This study demonstrates that the recombinant antigen generated and the technology used to produce it are suitable for developing a diagnostic tool against Infectious bursal disease.http://link.springer.com/article/10.1186/s13568-020-01029-zIBDVIn-house ELISAMolecular farmingRecombinant SVP |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Evangelina Gómez María Florencia Cassani María Soledad Lucero Viviana Parreño Silvina Chimeno Zoth Analía Berinstein |
spellingShingle |
Evangelina Gómez María Florencia Cassani María Soledad Lucero Viviana Parreño Silvina Chimeno Zoth Analía Berinstein Development of diagnostic tools for IBDV detection using plants as bioreactors AMB Express IBDV In-house ELISA Molecular farming Recombinant SVP |
author_facet |
Evangelina Gómez María Florencia Cassani María Soledad Lucero Viviana Parreño Silvina Chimeno Zoth Analía Berinstein |
author_sort |
Evangelina Gómez |
title |
Development of diagnostic tools for IBDV detection using plants as bioreactors |
title_short |
Development of diagnostic tools for IBDV detection using plants as bioreactors |
title_full |
Development of diagnostic tools for IBDV detection using plants as bioreactors |
title_fullStr |
Development of diagnostic tools for IBDV detection using plants as bioreactors |
title_full_unstemmed |
Development of diagnostic tools for IBDV detection using plants as bioreactors |
title_sort |
development of diagnostic tools for ibdv detection using plants as bioreactors |
publisher |
SpringerOpen |
series |
AMB Express |
issn |
2191-0855 |
publishDate |
2020-05-01 |
description |
Abstract Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana benthamiana. The SVP, which were observed by electronic microscopy, proved to be antigenically and immunogenically similar to the virion. Indeed, anti-IBDV antibodies from samples of infected birds recognized these SVP and, when injected intramuscularly, these subviral particles also evoked a humoral immune response in chickens. We developed an in-house ELISA using SVP as coating reagent that demonstrated to be highly accurate and in good agreement with a commercial ELISA. This study demonstrates that the recombinant antigen generated and the technology used to produce it are suitable for developing a diagnostic tool against Infectious bursal disease. |
topic |
IBDV In-house ELISA Molecular farming Recombinant SVP |
url |
http://link.springer.com/article/10.1186/s13568-020-01029-z |
work_keys_str_mv |
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