Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms.
The housefly feeds and reproduces in animal manure and decaying organic substances and thus lives in intimate association with various microorganisms including human pathogens. In order to understand the variation and association between bacteria and the housefly, we used 16S rRNA gene amplicon sequ...
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doaj-97a14f318621460289aca99a93f7a8552020-11-25T02:34:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01121e016975310.1371/journal.pone.0169753Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms.Simon BahrndorffNadieh de JongeHenrik SkovgårdJeppe Lund NielsenThe housefly feeds and reproduces in animal manure and decaying organic substances and thus lives in intimate association with various microorganisms including human pathogens. In order to understand the variation and association between bacteria and the housefly, we used 16S rRNA gene amplicon sequencing to describe bacterial communities of 90 individual houseflies collected within and between ten dairy farms in Denmark. Analysis of gene sequences showed that the most abundant classes of bacteria found across all sites included Bacilli, Clostridia, Actinobacteria, Flavobacteria, and all classes of Proteobacteria and at the genus level the most abundant genera included Corynebacterium, Lactobacillus, Staphylococcus, Vagococcus, Weissella, Lactococcus, and Aerococcus. Comparison of the microbiota of houseflies revealed a highly diverse microbiota compared to other insect species and with most variation in species richness and diversity found between individuals, but not locations. Our study is the first in-depth amplicon sequencing study of the housefly microbiota, and collectively shows that the microbiota of single houseflies is highly diverse and differs between individuals likely to reflect the lifestyle of the housefly. We suggest that these results should be taken into account when addressing the transmission of pathogens by the housefly and assessing the vector competence variation under natural conditions.http://europepmc.org/articles/PMC5232358?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Simon Bahrndorff Nadieh de Jonge Henrik Skovgård Jeppe Lund Nielsen |
spellingShingle |
Simon Bahrndorff Nadieh de Jonge Henrik Skovgård Jeppe Lund Nielsen Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms. PLoS ONE |
author_facet |
Simon Bahrndorff Nadieh de Jonge Henrik Skovgård Jeppe Lund Nielsen |
author_sort |
Simon Bahrndorff |
title |
Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms. |
title_short |
Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms. |
title_full |
Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms. |
title_fullStr |
Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms. |
title_full_unstemmed |
Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms. |
title_sort |
bacterial communities associated with houseflies (musca domestica l.) sampled within and between farms. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
The housefly feeds and reproduces in animal manure and decaying organic substances and thus lives in intimate association with various microorganisms including human pathogens. In order to understand the variation and association between bacteria and the housefly, we used 16S rRNA gene amplicon sequencing to describe bacterial communities of 90 individual houseflies collected within and between ten dairy farms in Denmark. Analysis of gene sequences showed that the most abundant classes of bacteria found across all sites included Bacilli, Clostridia, Actinobacteria, Flavobacteria, and all classes of Proteobacteria and at the genus level the most abundant genera included Corynebacterium, Lactobacillus, Staphylococcus, Vagococcus, Weissella, Lactococcus, and Aerococcus. Comparison of the microbiota of houseflies revealed a highly diverse microbiota compared to other insect species and with most variation in species richness and diversity found between individuals, but not locations. Our study is the first in-depth amplicon sequencing study of the housefly microbiota, and collectively shows that the microbiota of single houseflies is highly diverse and differs between individuals likely to reflect the lifestyle of the housefly. We suggest that these results should be taken into account when addressing the transmission of pathogens by the housefly and assessing the vector competence variation under natural conditions. |
url |
http://europepmc.org/articles/PMC5232358?pdf=render |
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