Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota
Dietary fibers are considered beneficial nutrients for health. Current data suggest that their interaction with the gut microbiota largely contributes to their physiological effects. In this context, chitin-glucan (CG) improves metabolic disorders associated with obesity in mice, but its effect on g...
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doaj-97a656bb983b4ca3aa88a63d53ca8aeb2021-03-18T15:12:50ZengTaylor & Francis GroupGut Microbes1949-09761949-09842020-11-0112110.1080/19490976.2020.18105301810530Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiotaJulie Rodriguez0Audrey M. Neyrinck1Zhengxiao Zhang2Benjamin Seethaler3Julie-Anne Nazare4Cándido Robles Sánchez5Martin Roumain6Giulio G. Muccioli7Laure B. Bindels8Patrice D. Cani9Véronique Maquet10Martine Laville11Stephan C. Bischoff12Jens Walter13Nathalie M. Delzenne14UCLouvain, Université Catholique de LouvainUCLouvain, Université Catholique de LouvainUniversity of AlbertaUniversity of HohenheimUniversité-Lyon, CarMeN Laboratory, Hospices Civils de LyonUCLouvain, Université Catholique de LouvainLouvain Drug Research Institute, UCLouvain, Université Catholique de LouvainLouvain Drug Research Institute, UCLouvain, Université Catholique de LouvainUCLouvain, Université Catholique de LouvainUCLouvain, Université Catholique de LouvainKitoZyme, Parc Industriel des Hauts-SartUniversité-Lyon, CarMeN Laboratory, Hospices Civils de LyonUniversity of HohenheimUniversity of AlbertaUCLouvain, Université Catholique de LouvainDietary fibers are considered beneficial nutrients for health. Current data suggest that their interaction with the gut microbiota largely contributes to their physiological effects. In this context, chitin-glucan (CG) improves metabolic disorders associated with obesity in mice, but its effect on gut microbiota has never been evaluated in humans. This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites. CG was given to healthy volunteers (n = 15) for three weeks as a supplement (4.5 g/day). Food diary, visual analog and Bristol stool form scales and a “quality of life” survey were analyzed. Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples. The gut microbiota (primary outcome) was analyzed by Illumina sequencing. A 3-week supplementation with CG is well tolerated in healthy humans. CG induces specific changes in the gut microbiota composition, with Eubacterium, Dorea and Roseburia genera showing the strongest regulation. In addition, CG increased bacterial metabolites in feces including butyric, iso-valeric, caproic and vaccenic acids. No major changes were observed for the fecal BA profile following CG intervention. In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota.http://dx.doi.org/10.1080/19490976.2020.1810530gut microbiotachitin-glucanfiberscfabile acids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julie Rodriguez Audrey M. Neyrinck Zhengxiao Zhang Benjamin Seethaler Julie-Anne Nazare Cándido Robles Sánchez Martin Roumain Giulio G. Muccioli Laure B. Bindels Patrice D. Cani Véronique Maquet Martine Laville Stephan C. Bischoff Jens Walter Nathalie M. Delzenne |
spellingShingle |
Julie Rodriguez Audrey M. Neyrinck Zhengxiao Zhang Benjamin Seethaler Julie-Anne Nazare Cándido Robles Sánchez Martin Roumain Giulio G. Muccioli Laure B. Bindels Patrice D. Cani Véronique Maquet Martine Laville Stephan C. Bischoff Jens Walter Nathalie M. Delzenne Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota Gut Microbes gut microbiota chitin-glucan fiber scfa bile acids |
author_facet |
Julie Rodriguez Audrey M. Neyrinck Zhengxiao Zhang Benjamin Seethaler Julie-Anne Nazare Cándido Robles Sánchez Martin Roumain Giulio G. Muccioli Laure B. Bindels Patrice D. Cani Véronique Maquet Martine Laville Stephan C. Bischoff Jens Walter Nathalie M. Delzenne |
author_sort |
Julie Rodriguez |
title |
Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota |
title_short |
Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota |
title_full |
Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota |
title_fullStr |
Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota |
title_full_unstemmed |
Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota |
title_sort |
metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota |
publisher |
Taylor & Francis Group |
series |
Gut Microbes |
issn |
1949-0976 1949-0984 |
publishDate |
2020-11-01 |
description |
Dietary fibers are considered beneficial nutrients for health. Current data suggest that their interaction with the gut microbiota largely contributes to their physiological effects. In this context, chitin-glucan (CG) improves metabolic disorders associated with obesity in mice, but its effect on gut microbiota has never been evaluated in humans. This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites. CG was given to healthy volunteers (n = 15) for three weeks as a supplement (4.5 g/day). Food diary, visual analog and Bristol stool form scales and a “quality of life” survey were analyzed. Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples. The gut microbiota (primary outcome) was analyzed by Illumina sequencing. A 3-week supplementation with CG is well tolerated in healthy humans. CG induces specific changes in the gut microbiota composition, with Eubacterium, Dorea and Roseburia genera showing the strongest regulation. In addition, CG increased bacterial metabolites in feces including butyric, iso-valeric, caproic and vaccenic acids. No major changes were observed for the fecal BA profile following CG intervention. In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota. |
topic |
gut microbiota chitin-glucan fiber scfa bile acids |
url |
http://dx.doi.org/10.1080/19490976.2020.1810530 |
work_keys_str_mv |
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