Development of an open hardware bioreactor for optimized cardiac cell culture integrating programmable mechanical and electrical stimulations

Development of an open hardware bioreactor for optimized cardiac cell culture integrating programmable mechanical and electrical stimulations

A new open-hardware bioreactor capable of applying electrical field stimulation in conjunction with static or cyclic stretch is presented. Stretch is applied to cells by a specially designed elastomeric membrane with a central seeding region. The main interest of our approach is the fine control of...

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Main Authors: Jonathan Béland, James Elber Duverger, Estelle Petitjean, Ange Maguy, Jonathan Ledoux, Philippe Comtois
Format: Article
Language:English
Published: AIP Publishing LLC 2020-03-01
Series:AIP Advances
Online Access:http://dx.doi.org/10.1063/1.5144922
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spelling doaj-97b892ce204143f78e5df7b9c30bfd912020-11-25T03:20:18ZengAIP Publishing LLCAIP Advances2158-32262020-03-01103035133035133-1110.1063/1.5144922Development of an open hardware bioreactor for optimized cardiac cell culture integrating programmable mechanical and electrical stimulationsJonathan Béland0James Elber Duverger1Estelle Petitjean2Ange Maguy3Jonathan Ledoux4Philippe Comtois5Research Center, Montreal Heart Institute, Montreal, Quebec H1T 1C8, CanadaResearch Center, Montreal Heart Institute, Montreal, Quebec H1T 1C8, CanadaResearch Center, Montreal Heart Institute, Montreal, Quebec H1T 1C8, CanadaResearch Center, Montreal Heart Institute, Montreal, Quebec H1T 1C8, CanadaResearch Center, Montreal Heart Institute, Montreal, Quebec H1T 1C8, CanadaResearch Center, Montreal Heart Institute, Montreal, Quebec H1T 1C8, CanadaA new open-hardware bioreactor capable of applying electrical field stimulation in conjunction with static or cyclic stretch is presented. Stretch is applied to cells by a specially designed elastomeric membrane with a central seeding region. The main interest of our approach is the fine control of the characteristics of stimulations in regard to timing and amplitude in a simple design based on affordable, easy to find components and 3D printable parts. Our approach opens the way to more complex protocols for electrical and/or mechanical stimulations, which are known important regulators of cardiac phenotypes.http://dx.doi.org/10.1063/1.5144922
collection DOAJ
language English
format Article
sources DOAJ
author Jonathan Béland
James Elber Duverger
Estelle Petitjean
Ange Maguy
Jonathan Ledoux
Philippe Comtois
spellingShingle Jonathan Béland
James Elber Duverger
Estelle Petitjean
Ange Maguy
Jonathan Ledoux
Philippe Comtois
Development of an open hardware bioreactor for optimized cardiac cell culture integrating programmable mechanical and electrical stimulations
AIP Advances
author_facet Jonathan Béland
James Elber Duverger
Estelle Petitjean
Ange Maguy
Jonathan Ledoux
Philippe Comtois
author_sort Jonathan Béland
title Development of an open hardware bioreactor for optimized cardiac cell culture integrating programmable mechanical and electrical stimulations
title_short Development of an open hardware bioreactor for optimized cardiac cell culture integrating programmable mechanical and electrical stimulations
title_full Development of an open hardware bioreactor for optimized cardiac cell culture integrating programmable mechanical and electrical stimulations
title_fullStr Development of an open hardware bioreactor for optimized cardiac cell culture integrating programmable mechanical and electrical stimulations
title_full_unstemmed Development of an open hardware bioreactor for optimized cardiac cell culture integrating programmable mechanical and electrical stimulations
title_sort development of an open hardware bioreactor for optimized cardiac cell culture integrating programmable mechanical and electrical stimulations
publisher AIP Publishing LLC
series AIP Advances
issn 2158-3226
publishDate 2020-03-01
description A new open-hardware bioreactor capable of applying electrical field stimulation in conjunction with static or cyclic stretch is presented. Stretch is applied to cells by a specially designed elastomeric membrane with a central seeding region. The main interest of our approach is the fine control of the characteristics of stimulations in regard to timing and amplitude in a simple design based on affordable, easy to find components and 3D printable parts. Our approach opens the way to more complex protocols for electrical and/or mechanical stimulations, which are known important regulators of cardiac phenotypes.
url http://dx.doi.org/10.1063/1.5144922
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AT jameselberduverger developmentofanopenhardwarebioreactorforoptimizedcardiaccellcultureintegratingprogrammablemechanicalandelectricalstimulations
AT estellepetitjean developmentofanopenhardwarebioreactorforoptimizedcardiaccellcultureintegratingprogrammablemechanicalandelectricalstimulations
AT angemaguy developmentofanopenhardwarebioreactorforoptimizedcardiaccellcultureintegratingprogrammablemechanicalandelectricalstimulations
AT jonathanledoux developmentofanopenhardwarebioreactorforoptimizedcardiaccellcultureintegratingprogrammablemechanicalandelectricalstimulations
AT philippecomtois developmentofanopenhardwarebioreactorforoptimizedcardiaccellcultureintegratingprogrammablemechanicalandelectricalstimulations
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