Evaluation of ocular prosthesis biofilm and anophthalmic cavity contamination after use of three cleansing solutions
In addition to an initial socket discomfort, ocular prosthesis (OP) installation may allow the adherence of fungi and/or bacteria due to the superficial characteristics of the prosthesis' material, use of inadequate cleansing solutions and methods, or because the void located between the intern...
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University of São Paulo
2007-02-01
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doaj-97c566412cbf43aead2a2187e2e762c42020-11-25T02:38:41ZengUniversity of São PauloJournal of Applied Oral Science1678-77571678-77652007-02-01151333810.1590/S1678-77572007000100008Evaluation of ocular prosthesis biofilm and anophthalmic cavity contamination after use of three cleansing solutionsRegina Márcia Zuccolotto Felippe ParanhosCarlos Henrique BatalhãoMarisa SempriniSimone Cecílio Hallak RegaloIzabel Yoko ItoMaria da Glória Chiarello de MattosIn addition to an initial socket discomfort, ocular prosthesis (OP) installation may allow the adherence of fungi and/or bacteria due to the superficial characteristics of the prosthesis' material, use of inadequate cleansing solutions and methods, or because the void located between the internal portion of the prosthesis and the anophthalmic cavity (AC) mucosa. Objective: The aim of this study was to evaluate OP biofilm formation and the level of contamination of the internal portion of the OP and the AC in 24 patients. Material and Methods: Material was collected from the AC at the beginning of the study and 15 days after cleansing of the OP with 3 cleansing solutions: a neutral liquid soap, a multiuse solution for contact lens (Complete) and 0.12% chlorhexidine (Periogard). The collected materials were sowed in Petri dishes containing selective media for aerobic and facultative microorganisms, specifically staphylococci (Hipersalt agar with egg yolk), aerobic microorganisms (Brain Heart Infusion Blood Agar), streptococci (Mitis salivarius Agar), gram-negative bacilli (MacConkey Agar) and yeasts (Chromagar CandidaTM), incubated at 35ºC or 37ºC and the number of colony forming units were counted. Data were analyzed statistically by ANOVA, Friedman's test and Spearman's correlation. Results: Aerobic microorganisms, gram-negative bacilli and S. aureus were found in the OP biofilm and in the AC. There was statistically significant difference (p<0.05) between the number of microorganisms before and after the use of the cleansing solutions. Conclusion: There was positive correlation with respect to the microorganisms present in the OP biofilm and AC for the 4 proposed treatments, indicating that the decrease of OP contamination leads to AC contamination as well.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572007000100008Artificial eyeEye infectionsProsthesis-related infections |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Regina Márcia Zuccolotto Felippe Paranhos Carlos Henrique Batalhão Marisa Semprini Simone Cecílio Hallak Regalo Izabel Yoko Ito Maria da Glória Chiarello de Mattos |
spellingShingle |
Regina Márcia Zuccolotto Felippe Paranhos Carlos Henrique Batalhão Marisa Semprini Simone Cecílio Hallak Regalo Izabel Yoko Ito Maria da Glória Chiarello de Mattos Evaluation of ocular prosthesis biofilm and anophthalmic cavity contamination after use of three cleansing solutions Journal of Applied Oral Science Artificial eye Eye infections Prosthesis-related infections |
author_facet |
Regina Márcia Zuccolotto Felippe Paranhos Carlos Henrique Batalhão Marisa Semprini Simone Cecílio Hallak Regalo Izabel Yoko Ito Maria da Glória Chiarello de Mattos |
author_sort |
Regina Márcia Zuccolotto Felippe Paranhos |
title |
Evaluation of ocular prosthesis biofilm and anophthalmic cavity contamination after use of three cleansing solutions |
title_short |
Evaluation of ocular prosthesis biofilm and anophthalmic cavity contamination after use of three cleansing solutions |
title_full |
Evaluation of ocular prosthesis biofilm and anophthalmic cavity contamination after use of three cleansing solutions |
title_fullStr |
Evaluation of ocular prosthesis biofilm and anophthalmic cavity contamination after use of three cleansing solutions |
title_full_unstemmed |
Evaluation of ocular prosthesis biofilm and anophthalmic cavity contamination after use of three cleansing solutions |
title_sort |
evaluation of ocular prosthesis biofilm and anophthalmic cavity contamination after use of three cleansing solutions |
publisher |
University of São Paulo |
series |
Journal of Applied Oral Science |
issn |
1678-7757 1678-7765 |
publishDate |
2007-02-01 |
description |
In addition to an initial socket discomfort, ocular prosthesis (OP) installation may allow the adherence of fungi and/or bacteria due to the superficial characteristics of the prosthesis' material, use of inadequate cleansing solutions and methods, or because the void located between the internal portion of the prosthesis and the anophthalmic cavity (AC) mucosa. Objective: The aim of this study was to evaluate OP biofilm formation and the level of contamination of the internal portion of the OP and the AC in 24 patients. Material and Methods: Material was collected from the AC at the beginning of the study and 15 days after cleansing of the OP with 3 cleansing solutions: a neutral liquid soap, a multiuse solution for contact lens (Complete) and 0.12% chlorhexidine (Periogard). The collected materials were sowed in Petri dishes containing selective media for aerobic and facultative microorganisms, specifically staphylococci (Hipersalt agar with egg yolk), aerobic microorganisms (Brain Heart Infusion Blood Agar), streptococci (Mitis salivarius Agar), gram-negative bacilli (MacConkey Agar) and yeasts (Chromagar CandidaTM), incubated at 35ºC or 37ºC and the number of colony forming units were counted. Data were analyzed statistically by ANOVA, Friedman's test and Spearman's correlation. Results: Aerobic microorganisms, gram-negative bacilli and S. aureus were found in the OP biofilm and in the AC. There was statistically significant difference (p<0.05) between the number of microorganisms before and after the use of the cleansing solutions. Conclusion: There was positive correlation with respect to the microorganisms present in the OP biofilm and AC for the 4 proposed treatments, indicating that the decrease of OP contamination leads to AC contamination as well. |
topic |
Artificial eye Eye infections Prosthesis-related infections |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572007000100008 |
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