A bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injection
A thioredoxin-like protein (txl) gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus txl (Bitxl) gene spans 1777 bp and consists of three introns and four exons coding for 285 amino acid residues with a conserved active site (CGPC). The deduced amino acid sequence of the Bitxl cDNA wa...
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Institute of Entomology, Biology Centre, Czech Academy of Science
2006-04-01
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doaj-97c7a07a55ff411a9456c4bd74ab4a9c2021-04-16T20:34:03ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292006-04-01103229129610.14411/eje.2006.037eje-200602-0002A bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injectionYong Soo CHOI0Kwang Sik LEE1Hyung Joo YOON2Iksoo KIM3Hung Dae SOHN4Byung Rae JIN5College of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaCollege of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaDepartment of Agricultural Biology, National Institute of Agricultural Science and Technology, Suwon 441-100, KoreaDepartment of Agricultural Biology, National Institute of Agricultural Science and Technology, Suwon 441-100, KoreaCollege of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaCollege of Natural Resources and Life Science, Dong-A University, Busan 604-714, KoreaA thioredoxin-like protein (txl) gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus txl (Bitxl) gene spans 1777 bp and consists of three introns and four exons coding for 285 amino acid residues with a conserved active site (CGPC). The deduced amino acid sequence of the Bitxl cDNA was 65% similar to the Drosophila melanogaster txl. Northern blot analysis revealed the presence of Bitxl transcripts in all tissues examined. When H2O2 was injected into the body cavity of B. ignitus workers, Bitxl mRNA expression was up-regulated in the fat body tissue. In addition, the expression levels of Bitxl mRNA in the fat body greatly increased when B. ignitus workers were exposed to low (4°C) or high (37°C) temperatures, or injected with lipopolysaccharide (LPS), which suggests that the Bitxl possibly protects against oxidative stress caused by extreme temperatures and bacterial infection.https://www.eje.cz/artkey/eje-200602-0002_A_bumblebee_thioredoxin-like_protein_gene_that_is_up-regulated_by_a_temperature_stimulus_and_lipopolysaccharide.phpantioxidant enzymeapidaebumblebeebombus ignitusgene structureoxidative stressreactive oxygen species (ros)thioredoxin-like protein (txl) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yong Soo CHOI Kwang Sik LEE Hyung Joo YOON Iksoo KIM Hung Dae SOHN Byung Rae JIN |
spellingShingle |
Yong Soo CHOI Kwang Sik LEE Hyung Joo YOON Iksoo KIM Hung Dae SOHN Byung Rae JIN A bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injection European Journal of Entomology antioxidant enzyme apidae bumblebee bombus ignitus gene structure oxidative stress reactive oxygen species (ros) thioredoxin-like protein (txl) |
author_facet |
Yong Soo CHOI Kwang Sik LEE Hyung Joo YOON Iksoo KIM Hung Dae SOHN Byung Rae JIN |
author_sort |
Yong Soo CHOI |
title |
A bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injection |
title_short |
A bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injection |
title_full |
A bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injection |
title_fullStr |
A bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injection |
title_full_unstemmed |
A bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injection |
title_sort |
bumblebee thioredoxin-like protein gene that is up-regulated by a temperature stimulus and lipopolysaccharide injection |
publisher |
Institute of Entomology, Biology Centre, Czech Academy of Science |
series |
European Journal of Entomology |
issn |
1210-5759 1802-8829 |
publishDate |
2006-04-01 |
description |
A thioredoxin-like protein (txl) gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus txl (Bitxl) gene spans 1777 bp and consists of three introns and four exons coding for 285 amino acid residues with a conserved active site (CGPC). The deduced amino acid sequence of the Bitxl cDNA was 65% similar to the Drosophila melanogaster txl. Northern blot analysis revealed the presence of Bitxl transcripts in all tissues examined. When H2O2 was injected into the body cavity of B. ignitus workers, Bitxl mRNA expression was up-regulated in the fat body tissue. In addition, the expression levels of Bitxl mRNA in the fat body greatly increased when B. ignitus workers were exposed to low (4°C) or high (37°C) temperatures, or injected with lipopolysaccharide (LPS), which suggests that the Bitxl possibly protects against oxidative stress caused by extreme temperatures and bacterial infection. |
topic |
antioxidant enzyme apidae bumblebee bombus ignitus gene structure oxidative stress reactive oxygen species (ros) thioredoxin-like protein (txl) |
url |
https://www.eje.cz/artkey/eje-200602-0002_A_bumblebee_thioredoxin-like_protein_gene_that_is_up-regulated_by_a_temperature_stimulus_and_lipopolysaccharide.php |
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