Characterization of ExeM, an Extracellular Nuclease of Shewanella oneidensis MR-1
Bacterial extracellular nucleases have multiple functions in processes as diverse as nutrient acquisition, natural transformation, biofilm formation, or defense against neutrophil extracellular traps (NETs). Here we explored the properties of ExeM in Shewanella oneidensis MR-1, an extracellular nucl...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2018-08-01
|
Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01761/full |
id |
doaj-97cf8089a57342e5a18fc57ca66091ac |
---|---|
record_format |
Article |
spelling |
doaj-97cf8089a57342e5a18fc57ca66091ac2020-11-25T01:58:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-08-01910.3389/fmicb.2018.01761379415Characterization of ExeM, an Extracellular Nuclease of Shewanella oneidensis MR-1Lucas BinnenkadeMaximilian KreienbaumKai M. ThormannBacterial extracellular nucleases have multiple functions in processes as diverse as nutrient acquisition, natural transformation, biofilm formation, or defense against neutrophil extracellular traps (NETs). Here we explored the properties of ExeM in Shewanella oneidensis MR-1, an extracellular nuclease, which is widely conserved among species of Shewanella, Vibrio, Aeromonas, and others. In S. oneidensis, ExeM is crucial for normal biofilm formation. In vitro activity measurements on heterologously produced ExeM revealed that this enzyme is a sugar-unspecific endonuclease, which requires Ca2+ and Mg2+/Mn2+ as co-factors for full activity. ExeM was almost exclusively localized to the cytoplasmic membrane fraction, even when a putative C-terminal membrane anchor was deleted. In contrast, ExeM was not detected in medium supernatants. Based on the results we hypothesize that ExeM predominantly interacts with DNA in close proximity to the cell, e.g., to promote biofilm formation and defense against NETs, or to control uptake of DNA.https://www.frontiersin.org/article/10.3389/fmicb.2018.01761/fullDNAbiofilmmembraneendonucleaseShewanella |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lucas Binnenkade Maximilian Kreienbaum Kai M. Thormann |
spellingShingle |
Lucas Binnenkade Maximilian Kreienbaum Kai M. Thormann Characterization of ExeM, an Extracellular Nuclease of Shewanella oneidensis MR-1 Frontiers in Microbiology DNA biofilm membrane endonuclease Shewanella |
author_facet |
Lucas Binnenkade Maximilian Kreienbaum Kai M. Thormann |
author_sort |
Lucas Binnenkade |
title |
Characterization of ExeM, an Extracellular Nuclease of Shewanella oneidensis MR-1 |
title_short |
Characterization of ExeM, an Extracellular Nuclease of Shewanella oneidensis MR-1 |
title_full |
Characterization of ExeM, an Extracellular Nuclease of Shewanella oneidensis MR-1 |
title_fullStr |
Characterization of ExeM, an Extracellular Nuclease of Shewanella oneidensis MR-1 |
title_full_unstemmed |
Characterization of ExeM, an Extracellular Nuclease of Shewanella oneidensis MR-1 |
title_sort |
characterization of exem, an extracellular nuclease of shewanella oneidensis mr-1 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-08-01 |
description |
Bacterial extracellular nucleases have multiple functions in processes as diverse as nutrient acquisition, natural transformation, biofilm formation, or defense against neutrophil extracellular traps (NETs). Here we explored the properties of ExeM in Shewanella oneidensis MR-1, an extracellular nuclease, which is widely conserved among species of Shewanella, Vibrio, Aeromonas, and others. In S. oneidensis, ExeM is crucial for normal biofilm formation. In vitro activity measurements on heterologously produced ExeM revealed that this enzyme is a sugar-unspecific endonuclease, which requires Ca2+ and Mg2+/Mn2+ as co-factors for full activity. ExeM was almost exclusively localized to the cytoplasmic membrane fraction, even when a putative C-terminal membrane anchor was deleted. In contrast, ExeM was not detected in medium supernatants. Based on the results we hypothesize that ExeM predominantly interacts with DNA in close proximity to the cell, e.g., to promote biofilm formation and defense against NETs, or to control uptake of DNA. |
topic |
DNA biofilm membrane endonuclease Shewanella |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2018.01761/full |
work_keys_str_mv |
AT lucasbinnenkade characterizationofexemanextracellularnucleaseofshewanellaoneidensismr1 AT maximiliankreienbaum characterizationofexemanextracellularnucleaseofshewanellaoneidensismr1 AT kaimthormann characterizationofexemanextracellularnucleaseofshewanellaoneidensismr1 |
_version_ |
1724969044873838592 |