Cytoplasmic and Nuclear Localization of TCTP in Normal and Cancer Cells
Objective. Intracellular localization of translationally controlled tumour protein (TCTP) was investigated in cancer cells. Methods. The expression and localization of TCTP were detected at 12 h, 24 h, 48 h, 60 h time points in culture of human hepatocarcinoma cell line HepG2, human cervical carcino...
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Series: | Biochemistry Research International |
Online Access: | http://dx.doi.org/10.1155/2012/871728 |
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doaj-97df9ca134724125a1ee3e661cad6dd32020-11-24T22:50:45ZengHindawi LimitedBiochemistry Research International2090-22472090-22552012-01-01201210.1155/2012/871728871728Cytoplasmic and Nuclear Localization of TCTP in Normal and Cancer CellsYu-Ping Ma0Wu-Ling Zhu1Department of Pathology, Xinxiang Medical University, Henan 453003, ChinaDepartment of Pathology, Xinxiang Medical University, Henan 453003, ChinaObjective. Intracellular localization of translationally controlled tumour protein (TCTP) was investigated in cancer cells. Methods. The expression and localization of TCTP were detected at 12 h, 24 h, 48 h, 60 h time points in culture of human hepatocarcinoma cell line HepG2, human cervical carcinoma cell line HeLa, and human normal liver cell line HL-7702 by immunofluorescence. Results. TCTP was expressed in both normal and tumor cells, and its localization changes at different time points. TCTP was mainly expressed in cytoplasm from 24 h to 48 h then expressed in both nucleus and cytoplasm at 60 h in HL-7702 cells. While in HepG2 cells, TCTP first localized at cell membrane within 24 h then at both nucleus and cytoplasm from 48 h to 60 h; TCTP localized at both nucleus and cytoplasm from 12 h to 60 h in Hela cells. Conclusion. The translocation of intracellular expression of TCTP in normal and tumor cells at different time points may pave a path to the studying of TCTP role in tumor growth.http://dx.doi.org/10.1155/2012/871728 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu-Ping Ma Wu-Ling Zhu |
spellingShingle |
Yu-Ping Ma Wu-Ling Zhu Cytoplasmic and Nuclear Localization of TCTP in Normal and Cancer Cells Biochemistry Research International |
author_facet |
Yu-Ping Ma Wu-Ling Zhu |
author_sort |
Yu-Ping Ma |
title |
Cytoplasmic and Nuclear Localization of TCTP in Normal and Cancer Cells |
title_short |
Cytoplasmic and Nuclear Localization of TCTP in Normal and Cancer Cells |
title_full |
Cytoplasmic and Nuclear Localization of TCTP in Normal and Cancer Cells |
title_fullStr |
Cytoplasmic and Nuclear Localization of TCTP in Normal and Cancer Cells |
title_full_unstemmed |
Cytoplasmic and Nuclear Localization of TCTP in Normal and Cancer Cells |
title_sort |
cytoplasmic and nuclear localization of tctp in normal and cancer cells |
publisher |
Hindawi Limited |
series |
Biochemistry Research International |
issn |
2090-2247 2090-2255 |
publishDate |
2012-01-01 |
description |
Objective. Intracellular localization of translationally controlled tumour protein (TCTP) was investigated in cancer cells. Methods. The expression and localization of TCTP were detected at 12 h, 24 h, 48 h, 60 h time points in culture of human hepatocarcinoma cell line HepG2, human cervical carcinoma cell line HeLa, and human normal liver cell line HL-7702 by immunofluorescence. Results. TCTP was expressed in both normal and tumor cells, and its localization changes at different time points. TCTP was mainly expressed in cytoplasm from 24 h to 48 h then expressed in both nucleus and cytoplasm at 60 h in HL-7702 cells. While in HepG2 cells, TCTP first localized at cell membrane within 24 h then at both nucleus and cytoplasm from 48 h to 60 h; TCTP localized at both nucleus and cytoplasm from 12 h to 60 h in Hela cells. Conclusion. The translocation of intracellular expression of TCTP in normal and tumor cells at different time points may pave a path to the studying of TCTP role in tumor growth. |
url |
http://dx.doi.org/10.1155/2012/871728 |
work_keys_str_mv |
AT yupingma cytoplasmicandnuclearlocalizationoftctpinnormalandcancercells AT wulingzhu cytoplasmicandnuclearlocalizationoftctpinnormalandcancercells |
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