<it>In vitro </it>and <it>in vivo </it>antitumor effects of acetylshikonin isolated from <it>Arnebia euchroma (Royle) Johnst </it>(<it>Ruanzicao</it>) cell suspension cultures
<p>Abstract</p> <p>Background</p> <p>Shikonin derivatives have cytotoxic and antitumor effects. This study aims to investigate the antitumor effects of acetylshikonin isolated from a Chinese medicinal herb <it>Arnebia euchroma (Royle) Johnst</it>.</p>...
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doaj-97f9fa6a18ce4757b661c4d4464a56062020-11-25T00:57:19ZengBMCChinese Medicine1749-85462009-07-01411410.1186/1749-8546-4-14<it>In vitro </it>and <it>in vivo </it>antitumor effects of acetylshikonin isolated from <it>Arnebia euchroma (Royle) Johnst </it>(<it>Ruanzicao</it>) cell suspension culturesZeng YunZhou LimingLuo GangXiong WenbiYang Wenji<p>Abstract</p> <p>Background</p> <p>Shikonin derivatives have cytotoxic and antitumor effects. This study aims to investigate the antitumor effects of acetylshikonin isolated from a Chinese medicinal herb <it>Arnebia euchroma (Royle) Johnst</it>.</p> <p>Methods</p> <p>The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the <it>in vitro </it>antitumor effects of acetylshikonin on human lung adenocarcinoma cell line A549, human hepatocellular carcinoma cell line Bel-7402, human breast adenocarcinoma cell line MCF-7 and mouse Lewis lung carcinoma (LLC) cell line. C<sub>57</sub>BL/6 mice with LLC model were used to study the <it>in vivo </it>antitumor effects of acetylshikonin. The expression of bax, bcl-2 and caspase-3 proteins in LLC tissue was determined with immunohistochemical staining.</p> <p>Results</p> <p>In A549, Bel-7402, MCF-7 and LLC cell lines, acetylshikonin inhibited cell growth in a dose-dependent manner. IC50 (means ± SD) were 5.6 ± 0.86 μg/ml, 6.82 ± 1.5 μg/ml, 3.04 ± 0.44 μg/ml and 2.72 ± 0.38 μg/ml respectively. Acetylshikonin suppressed tumor growth in C57BL/6 mice with LLC. The inhibition rate of acetylshikonin (2 mg/kg) was 42.85%. Immunohistochemical staining revealed that in the acetylshikonin groups the expression of bax and caspase-3 increased, whereas the expression of bcl-2 decreased, suggesting that acetylshikonin induced tumor cell apoptosis through activating the pro-apoptotic bcl-2 family and caspase-3.</p> <p>Conclusion</p> <p>Acetylshikonin isolated from <it>Arnebia euchroma (Royle) Johnst </it>cell suspension cultures exhibits specific <it>in vivo </it>and <it>in vitro </it>antitumor effects.</p> http://www.cmjournal.org/content/4/1/14 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zeng Yun Zhou Liming Luo Gang Xiong Wenbi Yang Wenji |
spellingShingle |
Zeng Yun Zhou Liming Luo Gang Xiong Wenbi Yang Wenji <it>In vitro </it>and <it>in vivo </it>antitumor effects of acetylshikonin isolated from <it>Arnebia euchroma (Royle) Johnst </it>(<it>Ruanzicao</it>) cell suspension cultures Chinese Medicine |
author_facet |
Zeng Yun Zhou Liming Luo Gang Xiong Wenbi Yang Wenji |
author_sort |
Zeng Yun |
title |
<it>In vitro </it>and <it>in vivo </it>antitumor effects of acetylshikonin isolated from <it>Arnebia euchroma (Royle) Johnst </it>(<it>Ruanzicao</it>) cell suspension cultures |
title_short |
<it>In vitro </it>and <it>in vivo </it>antitumor effects of acetylshikonin isolated from <it>Arnebia euchroma (Royle) Johnst </it>(<it>Ruanzicao</it>) cell suspension cultures |
title_full |
<it>In vitro </it>and <it>in vivo </it>antitumor effects of acetylshikonin isolated from <it>Arnebia euchroma (Royle) Johnst </it>(<it>Ruanzicao</it>) cell suspension cultures |
title_fullStr |
<it>In vitro </it>and <it>in vivo </it>antitumor effects of acetylshikonin isolated from <it>Arnebia euchroma (Royle) Johnst </it>(<it>Ruanzicao</it>) cell suspension cultures |
title_full_unstemmed |
<it>In vitro </it>and <it>in vivo </it>antitumor effects of acetylshikonin isolated from <it>Arnebia euchroma (Royle) Johnst </it>(<it>Ruanzicao</it>) cell suspension cultures |
title_sort |
<it>in vitro </it>and <it>in vivo </it>antitumor effects of acetylshikonin isolated from <it>arnebia euchroma (royle) johnst </it>(<it>ruanzicao</it>) cell suspension cultures |
publisher |
BMC |
series |
Chinese Medicine |
issn |
1749-8546 |
publishDate |
2009-07-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Shikonin derivatives have cytotoxic and antitumor effects. This study aims to investigate the antitumor effects of acetylshikonin isolated from a Chinese medicinal herb <it>Arnebia euchroma (Royle) Johnst</it>.</p> <p>Methods</p> <p>The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the <it>in vitro </it>antitumor effects of acetylshikonin on human lung adenocarcinoma cell line A549, human hepatocellular carcinoma cell line Bel-7402, human breast adenocarcinoma cell line MCF-7 and mouse Lewis lung carcinoma (LLC) cell line. C<sub>57</sub>BL/6 mice with LLC model were used to study the <it>in vivo </it>antitumor effects of acetylshikonin. The expression of bax, bcl-2 and caspase-3 proteins in LLC tissue was determined with immunohistochemical staining.</p> <p>Results</p> <p>In A549, Bel-7402, MCF-7 and LLC cell lines, acetylshikonin inhibited cell growth in a dose-dependent manner. IC50 (means ± SD) were 5.6 ± 0.86 μg/ml, 6.82 ± 1.5 μg/ml, 3.04 ± 0.44 μg/ml and 2.72 ± 0.38 μg/ml respectively. Acetylshikonin suppressed tumor growth in C57BL/6 mice with LLC. The inhibition rate of acetylshikonin (2 mg/kg) was 42.85%. Immunohistochemical staining revealed that in the acetylshikonin groups the expression of bax and caspase-3 increased, whereas the expression of bcl-2 decreased, suggesting that acetylshikonin induced tumor cell apoptosis through activating the pro-apoptotic bcl-2 family and caspase-3.</p> <p>Conclusion</p> <p>Acetylshikonin isolated from <it>Arnebia euchroma (Royle) Johnst </it>cell suspension cultures exhibits specific <it>in vivo </it>and <it>in vitro </it>antitumor effects.</p> |
url |
http://www.cmjournal.org/content/4/1/14 |
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