Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis
Abstract Dietary fatty acids play a role in the pathogenesis of obesity-associated non-alcoholic fatty liver disease (NAFLD), which is associated with insulin resistance (IR). Fatty acid composition is critical for IR and subsequent NAFLD development. Extra-virgin olive oil (EVOO) is the main source...
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Nature Publishing Group
2021-04-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-87761-3 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Leticia Álvarez-Amor Amparo Luque Sierra Antonio Cárdenas Lucía López-Bermudo Javier López-Beas Eloísa Andújar Mónica Pérez-Alegre Rocío Gallego-Durán Lourdes M. Varela Alejandro Martin-Montalvo Genoveva Berná Anabel Rojas Mª José Robles-Frías Abdelkrim Hmadcha Manuel Romero-Gómez Robert Kleemann Franz Martín |
spellingShingle |
Leticia Álvarez-Amor Amparo Luque Sierra Antonio Cárdenas Lucía López-Bermudo Javier López-Beas Eloísa Andújar Mónica Pérez-Alegre Rocío Gallego-Durán Lourdes M. Varela Alejandro Martin-Montalvo Genoveva Berná Anabel Rojas Mª José Robles-Frías Abdelkrim Hmadcha Manuel Romero-Gómez Robert Kleemann Franz Martín Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis Scientific Reports |
author_facet |
Leticia Álvarez-Amor Amparo Luque Sierra Antonio Cárdenas Lucía López-Bermudo Javier López-Beas Eloísa Andújar Mónica Pérez-Alegre Rocío Gallego-Durán Lourdes M. Varela Alejandro Martin-Montalvo Genoveva Berná Anabel Rojas Mª José Robles-Frías Abdelkrim Hmadcha Manuel Romero-Gómez Robert Kleemann Franz Martín |
author_sort |
Leticia Álvarez-Amor |
title |
Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis |
title_short |
Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis |
title_full |
Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis |
title_fullStr |
Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis |
title_full_unstemmed |
Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis |
title_sort |
extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese ldlr−/−.leiden mice without attenuation of steatohepatitis |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-04-01 |
description |
Abstract Dietary fatty acids play a role in the pathogenesis of obesity-associated non-alcoholic fatty liver disease (NAFLD), which is associated with insulin resistance (IR). Fatty acid composition is critical for IR and subsequent NAFLD development. Extra-virgin olive oil (EVOO) is the main source of monounsaturated fatty acids (MUFA) in Mediterranean diets. This study examined whether EVOO-containing high fat diets may prevent diet-induced NAFLD using Ldlr−/−. Leiden mice. In female Ldlr−/−.Leiden mice, the effects of the following high fat diets (HFDs) were examined: a lard-based HFD (HFD-L); an EVOO-based HFD (HFD-EVOO); a phenolic compounds-rich EVOO HFD (HFD-OL). We studied changes in body weight (BW), lipid profile, transaminases, glucose homeostasis, liver pathology and transcriptome. Both EVOO diets reduced body weight (BW) and improved insulin sensitivity. The EVOOs did not improve transaminase values and increased LDL-cholesterol and liver collagen content. EVOOs and HFD-L groups had comparable liver steatosis. The profibrotic effects were substantiated by an up-regulation of gene transcripts related to glutathione metabolism, chemokine signaling and NF-kappa-B activation and down-regulation of genes relevant for fatty acid metabolism. Collectivelly, EVOO intake improved weight gain and insulin sensitivity but not liver inflammation and fibrosis, which was supported by changes in hepatic genes expression. |
url |
https://doi.org/10.1038/s41598-021-87761-3 |
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doaj-98018cb0ec61418692f64df0f9c6509c2021-04-18T11:35:51ZengNature Publishing GroupScientific Reports2045-23222021-04-0111111610.1038/s41598-021-87761-3Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitisLeticia Álvarez-Amor0Amparo Luque Sierra1Antonio Cárdenas2Lucía López-Bermudo3Javier López-Beas4Eloísa Andújar5Mónica Pérez-Alegre6Rocío Gallego-Durán7Lourdes M. Varela8Alejandro Martin-Montalvo9Genoveva Berná10Anabel Rojas11Mª José Robles-Frías12Abdelkrim Hmadcha13Manuel Romero-Gómez14Robert Kleemann15Franz Martín16Andalusian Center of Molecular Biology and Regenerative Medicine-CABIMER, University Pablo Olavide-University of Seville-CSICAndalusian Center of Molecular Biology and Regenerative Medicine-CABIMER, University Pablo Olavide-University of Seville-CSICAndalusian Center of Molecular Biology and Regenerative Medicine-CABIMER, University Pablo Olavide-University of Seville-CSICAndalusian Center of Molecular Biology and Regenerative Medicine-CABIMER, University Pablo Olavide-University of Seville-CSICAndalusian Center of Molecular Biology and Regenerative Medicine-CABIMER, University Pablo Olavide-University of Seville-CSICAndalusian Center of Molecular Biology and Regenerative Medicine-CABIMER, University Pablo Olavide-University of Seville-CSICAndalusian Center of Molecular Biology and Regenerative Medicine-CABIMER, University Pablo Olavide-University of Seville-CSICHospital Universitario Virgen del Rocío de Sevilla, Instituto de Biomedicina de Sevilla, Universidad de SevillaAndalusian Center of Molecular Biology and Regenerative Medicine-CABIMER, University Pablo Olavide-University of Seville-CSICAndalusian Center of Molecular Biology and Regenerative Medicine-CABIMER, University Pablo Olavide-University of Seville-CSICAndalusian Center of Molecular Biology and Regenerative Medicine-CABIMER, University Pablo Olavide-University of Seville-CSICAndalusian Center of Molecular Biology and Regenerative Medicine-CABIMER, University Pablo Olavide-University of Seville-CSICCompared Pathology Unit, Hospital Universitario Virgen del Rocío-Intituto de Biomedicina de Sevilla Biobank Node, Andalucia Health Public System BiobankBiomedical Research Network On Diabetes and Related Metabolic Diseases-CIBERDEM, Instituto de Salud Carlos IIIHospital Universitario Virgen del Rocío de Sevilla, Instituto de Biomedicina de Sevilla, Universidad de SevillaDepartment of Metabolic Health Research, Netherlands Organisation for Applied Scientific Research-TNOAndalusian Center of Molecular Biology and Regenerative Medicine-CABIMER, University Pablo Olavide-University of Seville-CSICAbstract Dietary fatty acids play a role in the pathogenesis of obesity-associated non-alcoholic fatty liver disease (NAFLD), which is associated with insulin resistance (IR). Fatty acid composition is critical for IR and subsequent NAFLD development. Extra-virgin olive oil (EVOO) is the main source of monounsaturated fatty acids (MUFA) in Mediterranean diets. This study examined whether EVOO-containing high fat diets may prevent diet-induced NAFLD using Ldlr−/−. Leiden mice. In female Ldlr−/−.Leiden mice, the effects of the following high fat diets (HFDs) were examined: a lard-based HFD (HFD-L); an EVOO-based HFD (HFD-EVOO); a phenolic compounds-rich EVOO HFD (HFD-OL). We studied changes in body weight (BW), lipid profile, transaminases, glucose homeostasis, liver pathology and transcriptome. Both EVOO diets reduced body weight (BW) and improved insulin sensitivity. The EVOOs did not improve transaminase values and increased LDL-cholesterol and liver collagen content. EVOOs and HFD-L groups had comparable liver steatosis. The profibrotic effects were substantiated by an up-regulation of gene transcripts related to glutathione metabolism, chemokine signaling and NF-kappa-B activation and down-regulation of genes relevant for fatty acid metabolism. Collectivelly, EVOO intake improved weight gain and insulin sensitivity but not liver inflammation and fibrosis, which was supported by changes in hepatic genes expression.https://doi.org/10.1038/s41598-021-87761-3 |