Effect of Novel Compound LX519290, a Derivative of l-allo Threonine, on Antioxidant Potential in Vitro and in Vivo
We investigated the antioxidative activity of LX519290, a derivative of l-allo threonine, in vitro and in vivo. To evaluate the antioxidative activity of LX519290, we performed several in vitro assays (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2016-09-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | http://www.mdpi.com/1422-0067/17/9/1451 |
id |
doaj-9817f017a07a480abd72836eb0f75764 |
---|---|
record_format |
Article |
spelling |
doaj-9817f017a07a480abd72836eb0f757642020-11-24T21:52:01ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-09-01179145110.3390/ijms17091451ijms17091451Effect of Novel Compound LX519290, a Derivative of l-allo Threonine, on Antioxidant Potential in Vitro and in VivoKun Chun0Md Badrul Alam1Hyeong-U Son2Sang-Han Lee3Department of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, KoreaDepartment of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, KoreaDepartment of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, KoreaDepartment of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, KoreaWe investigated the antioxidative activity of LX519290, a derivative of l-allo threonine, in vitro and in vivo. To evaluate the antioxidative activity of LX519290, we performed several in vitro assays (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical-scavenging assays, a ferric reducing antioxidant power assay, cupric-reducing antioxidant capacity, and oxygen radical absorbance capacity assay) and evaluated inhibition against the generation of nitric oxide (NO) and reactive oxygen species (ROS) in murine macrophage (RAW264.7) cells. The results showed that LX519290 possessed very strong radical scavenging activity and reducing power, and inhibited NO and ROS generation in a dose-dependent manner without showing any cytotoxicity. LX519290 treatment also increased the total thiol content and glutathione S-transferases (GST) activities in RAW264.7 cells. Finally, we also determined whether LX519290 affects the mRNA levels of antioxidant enzymes in vitro and in vivo. The expression of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were markedly higher in the sample-treated group than in the oxidative stress group. LX519290 treatment also increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) with corresponding increases in the transcriptional and translational activities of haeme oxygenase-1 (HO-1). Collectively, the data demonstrated that LX519290 has potent antioxidative activity, decreases NO and ROS generation, increases total thiol content and GST activities in RAW264.7 cells, and increases the transcriptional and translational levels of antioxidant enzymes in vitro and in vivo.http://www.mdpi.com/1422-0067/17/9/1451ʟ-allo threonineLX519290antioxidative activityin vitroin vivo |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kun Chun Md Badrul Alam Hyeong-U Son Sang-Han Lee |
spellingShingle |
Kun Chun Md Badrul Alam Hyeong-U Son Sang-Han Lee Effect of Novel Compound LX519290, a Derivative of l-allo Threonine, on Antioxidant Potential in Vitro and in Vivo International Journal of Molecular Sciences ʟ-allo threonine LX519290 antioxidative activity in vitro in vivo |
author_facet |
Kun Chun Md Badrul Alam Hyeong-U Son Sang-Han Lee |
author_sort |
Kun Chun |
title |
Effect of Novel Compound LX519290, a Derivative of l-allo Threonine, on Antioxidant Potential in Vitro and in Vivo |
title_short |
Effect of Novel Compound LX519290, a Derivative of l-allo Threonine, on Antioxidant Potential in Vitro and in Vivo |
title_full |
Effect of Novel Compound LX519290, a Derivative of l-allo Threonine, on Antioxidant Potential in Vitro and in Vivo |
title_fullStr |
Effect of Novel Compound LX519290, a Derivative of l-allo Threonine, on Antioxidant Potential in Vitro and in Vivo |
title_full_unstemmed |
Effect of Novel Compound LX519290, a Derivative of l-allo Threonine, on Antioxidant Potential in Vitro and in Vivo |
title_sort |
effect of novel compound lx519290, a derivative of l-allo threonine, on antioxidant potential in vitro and in vivo |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2016-09-01 |
description |
We investigated the antioxidative activity of LX519290, a derivative of l-allo threonine, in vitro and in vivo. To evaluate the antioxidative activity of LX519290, we performed several in vitro assays (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical-scavenging assays, a ferric reducing antioxidant power assay, cupric-reducing antioxidant capacity, and oxygen radical absorbance capacity assay) and evaluated inhibition against the generation of nitric oxide (NO) and reactive oxygen species (ROS) in murine macrophage (RAW264.7) cells. The results showed that LX519290 possessed very strong radical scavenging activity and reducing power, and inhibited NO and ROS generation in a dose-dependent manner without showing any cytotoxicity. LX519290 treatment also increased the total thiol content and glutathione S-transferases (GST) activities in RAW264.7 cells. Finally, we also determined whether LX519290 affects the mRNA levels of antioxidant enzymes in vitro and in vivo. The expression of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were markedly higher in the sample-treated group than in the oxidative stress group. LX519290 treatment also increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) with corresponding increases in the transcriptional and translational activities of haeme oxygenase-1 (HO-1). Collectively, the data demonstrated that LX519290 has potent antioxidative activity, decreases NO and ROS generation, increases total thiol content and GST activities in RAW264.7 cells, and increases the transcriptional and translational levels of antioxidant enzymes in vitro and in vivo. |
topic |
ʟ-allo threonine LX519290 antioxidative activity in vitro in vivo |
url |
http://www.mdpi.com/1422-0067/17/9/1451 |
work_keys_str_mv |
AT kunchun effectofnovelcompoundlx519290aderivativeoflallothreonineonantioxidantpotentialinvitroandinvivo AT mdbadrulalam effectofnovelcompoundlx519290aderivativeoflallothreonineonantioxidantpotentialinvitroandinvivo AT hyeonguson effectofnovelcompoundlx519290aderivativeoflallothreonineonantioxidantpotentialinvitroandinvivo AT sanghanlee effectofnovelcompoundlx519290aderivativeoflallothreonineonantioxidantpotentialinvitroandinvivo |
_version_ |
1725877333398650880 |