Angiomotin functions in HIV-1 assembly and budding
Many retroviral Gag proteins contain PPXY late assembly domain motifs that recruit proteins of the NEDD4 E3 ubiquitin ligase family to facilitate virus release. Overexpression of NEDD4L can also stimulate HIV-1 release but in this case the Gag protein lacks a PPXY motif, suggesting that NEDD4L may f...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2015-01-01
|
Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/03778 |
id |
doaj-983ae360e41f45409ecc2ed7090babbb |
---|---|
record_format |
Article |
spelling |
doaj-983ae360e41f45409ecc2ed7090babbb2021-05-04T23:38:22ZengeLife Sciences Publications LtdeLife2050-084X2015-01-01410.7554/eLife.03778Angiomotin functions in HIV-1 assembly and buddingGaelle Mercenne0Steven L Alam1Jun Arii2Matthew S Lalonde3Wesley I Sundquist4Department of Biochemistry, University of Utah, Salt Lake City, United StatesDepartment of Biochemistry, University of Utah, Salt Lake City, United StatesDepartment of Biochemistry, University of Utah, Salt Lake City, United StatesDepartment of Biochemistry, University of Utah, Salt Lake City, United StatesDepartment of Biochemistry, University of Utah, Salt Lake City, United StatesMany retroviral Gag proteins contain PPXY late assembly domain motifs that recruit proteins of the NEDD4 E3 ubiquitin ligase family to facilitate virus release. Overexpression of NEDD4L can also stimulate HIV-1 release but in this case the Gag protein lacks a PPXY motif, suggesting that NEDD4L may function through an adaptor protein. Here, we demonstrate that the cellular protein Angiomotin (AMOT) can bind both NEDD4L and HIV-1 Gag. HIV-1 release and infectivity are stimulated by AMOT overexpression and inhibited by AMOT depletion, whereas AMOT mutants that cannot bind NEDD4L cannot function in virus release. Electron microscopic analyses revealed that in the absence of AMOT assembling Gag molecules fail to form a fully spherical enveloped particle. Our experiments indicate that AMOT and other motin family members function together with NEDD4L to help complete immature virion assembly prior to ESCRT-mediated virus budding.https://elifesciences.org/articles/03778HIV Gag proteinNEDD4Lmotin protein familyESCRT pathwayvirus-host interaction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gaelle Mercenne Steven L Alam Jun Arii Matthew S Lalonde Wesley I Sundquist |
spellingShingle |
Gaelle Mercenne Steven L Alam Jun Arii Matthew S Lalonde Wesley I Sundquist Angiomotin functions in HIV-1 assembly and budding eLife HIV Gag protein NEDD4L motin protein family ESCRT pathway virus-host interaction |
author_facet |
Gaelle Mercenne Steven L Alam Jun Arii Matthew S Lalonde Wesley I Sundquist |
author_sort |
Gaelle Mercenne |
title |
Angiomotin functions in HIV-1 assembly and budding |
title_short |
Angiomotin functions in HIV-1 assembly and budding |
title_full |
Angiomotin functions in HIV-1 assembly and budding |
title_fullStr |
Angiomotin functions in HIV-1 assembly and budding |
title_full_unstemmed |
Angiomotin functions in HIV-1 assembly and budding |
title_sort |
angiomotin functions in hiv-1 assembly and budding |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2015-01-01 |
description |
Many retroviral Gag proteins contain PPXY late assembly domain motifs that recruit proteins of the NEDD4 E3 ubiquitin ligase family to facilitate virus release. Overexpression of NEDD4L can also stimulate HIV-1 release but in this case the Gag protein lacks a PPXY motif, suggesting that NEDD4L may function through an adaptor protein. Here, we demonstrate that the cellular protein Angiomotin (AMOT) can bind both NEDD4L and HIV-1 Gag. HIV-1 release and infectivity are stimulated by AMOT overexpression and inhibited by AMOT depletion, whereas AMOT mutants that cannot bind NEDD4L cannot function in virus release. Electron microscopic analyses revealed that in the absence of AMOT assembling Gag molecules fail to form a fully spherical enveloped particle. Our experiments indicate that AMOT and other motin family members function together with NEDD4L to help complete immature virion assembly prior to ESCRT-mediated virus budding. |
topic |
HIV Gag protein NEDD4L motin protein family ESCRT pathway virus-host interaction |
url |
https://elifesciences.org/articles/03778 |
work_keys_str_mv |
AT gaellemercenne angiomotinfunctionsinhiv1assemblyandbudding AT stevenlalam angiomotinfunctionsinhiv1assemblyandbudding AT junarii angiomotinfunctionsinhiv1assemblyandbudding AT matthewslalonde angiomotinfunctionsinhiv1assemblyandbudding AT wesleyisundquist angiomotinfunctionsinhiv1assemblyandbudding |
_version_ |
1721476879270019072 |