Patch-clamp recordings of rat neurons from acute brain slices of the somatosensory cortex during magnetic stimulation
Although transcranial magnetic stimulation (TMS) is a popular tool for both basic research and clinical applications, its actions on nerve cells are only partially understood. We have previously predicted, using compartmental modeling, that magnetic stimulation of central nervous system neurons depo...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2014-06-01
|
Series: | Frontiers in Cellular Neuroscience |
Subjects: | |
Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00145/full |
id |
doaj-983be985f8ce4114a1f5bc4dd3a5b379 |
---|---|
record_format |
Article |
spelling |
doaj-983be985f8ce4114a1f5bc4dd3a5b3792020-11-24T22:56:10ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022014-06-01810.3389/fncel.2014.0014589435Patch-clamp recordings of rat neurons from acute brain slices of the somatosensory cortex during magnetic stimulationTamar ePashut0Dafna eMagidov1Hana eBen-Porat2Shuki eWolfus3Alex eFriedman4Eli ePerel5Michal eLavidor6Izhar eBar‐Gad7Yosef eYeshurun8Alon eKorngreen9Bar-Ilan UniversityBar-Ilan UniversityBar-Ilan UniversityBar-Ilan UniversityBar-Ilan UniversityBar-Ilan UniversityBar-Ilan UniversityBar-Ilan UniversityBar-Ilan UniversityBar-Ilan UniversityAlthough transcranial magnetic stimulation (TMS) is a popular tool for both basic research and clinical applications, its actions on nerve cells are only partially understood. We have previously predicted, using compartmental modeling, that magnetic stimulation of central nervous system neurons depolarized the soma followed by initiation of an action potential in the initial segment of the axon. The simulations also predict that neurons with low current threshold are more susceptible to magnetic stimulation. Here we tested these theoretical predictions by combining in vitro patch-clamp recordings from rat brain slices with magnetic stimulation and compartmental modeling. In agreement with the modeling, our recordings demonstrate the dependence of magnetic stimulation-triggered action potentials on the type and state of the neuron and its orientation within the magnetic field. Our results suggest that the observed effects of TMS are deeply rooted in the biophysical properties of single neurons in the central nervous system and provide a framework both for interpreting existing TMS data and developing new simulation-based tools and therapies.http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00145/fullMembrane PotentialsCortexTMSIn-vitromagnetic stimulationpatch-clamp |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tamar ePashut Dafna eMagidov Hana eBen-Porat Shuki eWolfus Alex eFriedman Eli ePerel Michal eLavidor Izhar eBar‐Gad Yosef eYeshurun Alon eKorngreen |
spellingShingle |
Tamar ePashut Dafna eMagidov Hana eBen-Porat Shuki eWolfus Alex eFriedman Eli ePerel Michal eLavidor Izhar eBar‐Gad Yosef eYeshurun Alon eKorngreen Patch-clamp recordings of rat neurons from acute brain slices of the somatosensory cortex during magnetic stimulation Frontiers in Cellular Neuroscience Membrane Potentials Cortex TMS In-vitro magnetic stimulation patch-clamp |
author_facet |
Tamar ePashut Dafna eMagidov Hana eBen-Porat Shuki eWolfus Alex eFriedman Eli ePerel Michal eLavidor Izhar eBar‐Gad Yosef eYeshurun Alon eKorngreen |
author_sort |
Tamar ePashut |
title |
Patch-clamp recordings of rat neurons from acute brain slices of the somatosensory cortex during magnetic stimulation |
title_short |
Patch-clamp recordings of rat neurons from acute brain slices of the somatosensory cortex during magnetic stimulation |
title_full |
Patch-clamp recordings of rat neurons from acute brain slices of the somatosensory cortex during magnetic stimulation |
title_fullStr |
Patch-clamp recordings of rat neurons from acute brain slices of the somatosensory cortex during magnetic stimulation |
title_full_unstemmed |
Patch-clamp recordings of rat neurons from acute brain slices of the somatosensory cortex during magnetic stimulation |
title_sort |
patch-clamp recordings of rat neurons from acute brain slices of the somatosensory cortex during magnetic stimulation |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular Neuroscience |
issn |
1662-5102 |
publishDate |
2014-06-01 |
description |
Although transcranial magnetic stimulation (TMS) is a popular tool for both basic research and clinical applications, its actions on nerve cells are only partially understood. We have previously predicted, using compartmental modeling, that magnetic stimulation of central nervous system neurons depolarized the soma followed by initiation of an action potential in the initial segment of the axon. The simulations also predict that neurons with low current threshold are more susceptible to magnetic stimulation. Here we tested these theoretical predictions by combining in vitro patch-clamp recordings from rat brain slices with magnetic stimulation and compartmental modeling. In agreement with the modeling, our recordings demonstrate the dependence of magnetic stimulation-triggered action potentials on the type and state of the neuron and its orientation within the magnetic field. Our results suggest that the observed effects of TMS are deeply rooted in the biophysical properties of single neurons in the central nervous system and provide a framework both for interpreting existing TMS data and developing new simulation-based tools and therapies. |
topic |
Membrane Potentials Cortex TMS In-vitro magnetic stimulation patch-clamp |
url |
http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00145/full |
work_keys_str_mv |
AT tamarepashut patchclamprecordingsofratneuronsfromacutebrainslicesofthesomatosensorycortexduringmagneticstimulation AT dafnaemagidov patchclamprecordingsofratneuronsfromacutebrainslicesofthesomatosensorycortexduringmagneticstimulation AT hanaebenporat patchclamprecordingsofratneuronsfromacutebrainslicesofthesomatosensorycortexduringmagneticstimulation AT shukiewolfus patchclamprecordingsofratneuronsfromacutebrainslicesofthesomatosensorycortexduringmagneticstimulation AT alexefriedman patchclamprecordingsofratneuronsfromacutebrainslicesofthesomatosensorycortexduringmagneticstimulation AT elieperel patchclamprecordingsofratneuronsfromacutebrainslicesofthesomatosensorycortexduringmagneticstimulation AT michalelavidor patchclamprecordingsofratneuronsfromacutebrainslicesofthesomatosensorycortexduringmagneticstimulation AT izharebargad patchclamprecordingsofratneuronsfromacutebrainslicesofthesomatosensorycortexduringmagneticstimulation AT yosefeyeshurun patchclamprecordingsofratneuronsfromacutebrainslicesofthesomatosensorycortexduringmagneticstimulation AT alonekorngreen patchclamprecordingsofratneuronsfromacutebrainslicesofthesomatosensorycortexduringmagneticstimulation |
_version_ |
1725654614356787200 |