Chloroquine-Resistant Haplotype Plasmodium falciparum Parasites, Haiti

• Main Authors: Berlin L. Londono, Thomas P. Eisele, Joseph Keating, Adam Bennett, Chandon Chattopadhyay, Gaetan Heyliger, Brian Mack, Ian Rawson, Jean-Francois Vely, Olbeg Désinor, Donald J. Krogstad
• Format: Article
• Language: English
• Published: Centers for Disease Control and Prevention 2009-05-01
• Series: Emerging Infectious Diseases
• Subjects: Malaria; parasites; Plasmodium falciparum; Haiti; chloroquine; P. falciparum chloroquine resistance transporter
• Online Access: https://wwwnc.cdc.gov/eid/article/15/5/08-1063_article

Plasmodium falciparum parasites have been endemic to Haiti for >40 years without evidence of chloroquine (CQ) resistance. In 2006 and 2007, we obtained blood smears for rapid diagnostic tests (RDTs) and filter paper blots of blood from 821 persons by passive and active case detection. P. falciparum infections diagnosed for 79 persons by blood smear or RDT were confirmed by PCR for the small subunit rRNA gene of P. falciparum. Amplification of the P. falciparum CQ resistance transporter (pfcrt) gene yielded 10 samples with amplicons resistant to cleavage by ApoI. A total of 5 of 9 samples had threonine at position 76 of pfcrt, which is consistent with CQ resistance (haplotypes at positions 72–76 were CVIET [n = 4] and CVMNT [n = 1]); 4 had only the wild-type haplotype associated with CQ susceptibility (CVMNK). These results indicate that CQ-resistant haplotype P. falciparum malaria parasites are present in Haiti.