Fusion of purple membranes triggered by immobilization on carbon nanomembranes
A freestanding ultrathin hybrid membrane was synthesized comprising two functional layers, that is, first, a carbon nanomembrane (CNM) produced by electron irradiation-induced cross-linking of a self-assembled monolayer (SAM) of 4′-nitro-1,1′-biphenyl-4-thiol (NBPT) and second, purple membrane (PM)...
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doaj-985382d04d5b4855be38b17915aeb4d02021-03-09T13:46:18ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862021-01-011219310110.3762/bjnano.12.82190-4286-12-8Fusion of purple membranes triggered by immobilization on carbon nanomembranesRené Riedel0Natalie Frese1Fang Yang2Martin Wortmann3Raphael Dalpke4Daniel Rhinow5Norbert Hampp6Armin Gölzhäuser7Faculty of Chemistry and Materials Sciences Center, University of Marburg, Hans-Meerwein-Strasse, D-35032 Marburg, GermanyPhysics of Supramolecular Systems and Surfaces, Faculty of Physics, Bielefeld University, Universitätsstraße 25, D-33615 Bielefeld, GermanyNano Biomaterials Group, Ningbo Institute of Industrial Technology, Chinese Academy of Science, ChinaFaculty of Engineering and Mathematics, Bielefeld University of Applied Sciences, Interaktion 1, D-33619 Bielefeld, GermanyPhysics of Supramolecular Systems and Surfaces, Faculty of Physics, Bielefeld University, Universitätsstraße 25, D-33615 Bielefeld, GermanyFaculty of Chemistry and Materials Sciences Center, University of Marburg, Hans-Meerwein-Strasse, D-35032 Marburg, GermanyFaculty of Chemistry and Materials Sciences Center, University of Marburg, Hans-Meerwein-Strasse, D-35032 Marburg, GermanyPhysics of Supramolecular Systems and Surfaces, Faculty of Physics, Bielefeld University, Universitätsstraße 25, D-33615 Bielefeld, GermanyA freestanding ultrathin hybrid membrane was synthesized comprising two functional layers, that is, first, a carbon nanomembrane (CNM) produced by electron irradiation-induced cross-linking of a self-assembled monolayer (SAM) of 4′-nitro-1,1′-biphenyl-4-thiol (NBPT) and second, purple membrane (PM) containing genetically modified bacteriorhodopsin (BR) carrying a C-terminal His-tag. The NBPT-CNM was further modified to carry nitrilotriacetic acid (NTA) terminal groups for the interaction with the His-tagged PMs forming a quasi-monolayer of His-tagged PM on top of the CNM-NTA. The formation of the Ni-NTA/His-tag complex leads to the unidirectional orientation of PM on the CNM substrate. Electrophoretic sedimentation was employed to optimize the surface coverage and to close gaps between the PM patches. This procedure for the immobilization of oriented dense PM facilitates the spontaneous fusion of individual PM patches, forming larger membrane areas. This is, to our knowledge, the very first procedure described to induce the oriented fusion of PM on a solid support. The resulting hybrid membrane has a potential application as a light-driven two-dimensional proton-pumping membrane, for instance, for light-driven seawater desalination as envisioned soon after the discovery of PM.https://doi.org/10.3762/bjnano.12.8bacteriorhodopsincarbon nanomembraneelectrophoretic sedimentationproton pumppurple membrane |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
René Riedel Natalie Frese Fang Yang Martin Wortmann Raphael Dalpke Daniel Rhinow Norbert Hampp Armin Gölzhäuser |
spellingShingle |
René Riedel Natalie Frese Fang Yang Martin Wortmann Raphael Dalpke Daniel Rhinow Norbert Hampp Armin Gölzhäuser Fusion of purple membranes triggered by immobilization on carbon nanomembranes Beilstein Journal of Nanotechnology bacteriorhodopsin carbon nanomembrane electrophoretic sedimentation proton pump purple membrane |
author_facet |
René Riedel Natalie Frese Fang Yang Martin Wortmann Raphael Dalpke Daniel Rhinow Norbert Hampp Armin Gölzhäuser |
author_sort |
René Riedel |
title |
Fusion of purple membranes triggered by immobilization on carbon nanomembranes |
title_short |
Fusion of purple membranes triggered by immobilization on carbon nanomembranes |
title_full |
Fusion of purple membranes triggered by immobilization on carbon nanomembranes |
title_fullStr |
Fusion of purple membranes triggered by immobilization on carbon nanomembranes |
title_full_unstemmed |
Fusion of purple membranes triggered by immobilization on carbon nanomembranes |
title_sort |
fusion of purple membranes triggered by immobilization on carbon nanomembranes |
publisher |
Beilstein-Institut |
series |
Beilstein Journal of Nanotechnology |
issn |
2190-4286 |
publishDate |
2021-01-01 |
description |
A freestanding ultrathin hybrid membrane was synthesized comprising two functional layers, that is, first, a carbon nanomembrane (CNM) produced by electron irradiation-induced cross-linking of a self-assembled monolayer (SAM) of 4′-nitro-1,1′-biphenyl-4-thiol (NBPT) and second, purple membrane (PM) containing genetically modified bacteriorhodopsin (BR) carrying a C-terminal His-tag. The NBPT-CNM was further modified to carry nitrilotriacetic acid (NTA) terminal groups for the interaction with the His-tagged PMs forming a quasi-monolayer of His-tagged PM on top of the CNM-NTA. The formation of the Ni-NTA/His-tag complex leads to the unidirectional orientation of PM on the CNM substrate. Electrophoretic sedimentation was employed to optimize the surface coverage and to close gaps between the PM patches. This procedure for the immobilization of oriented dense PM facilitates the spontaneous fusion of individual PM patches, forming larger membrane areas. This is, to our knowledge, the very first procedure described to induce the oriented fusion of PM on a solid support. The resulting hybrid membrane has a potential application as a light-driven two-dimensional proton-pumping membrane, for instance, for light-driven seawater desalination as envisioned soon after the discovery of PM. |
topic |
bacteriorhodopsin carbon nanomembrane electrophoretic sedimentation proton pump purple membrane |
url |
https://doi.org/10.3762/bjnano.12.8 |
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