EpCAM Induction Functionally Links to the Wnt-Enhanced Cell Proliferation in Human Keratinocytes
Accelerating proliferation of primary keratinocytes benefits skin autografts for severely burned patients. Wnt signal, a conserved pathway controlling cell cycle and morphogenesis in embryo, also involves in cell proliferation and tumorigenesis in adult tissues. Here the effects of Wnt signal on the...
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2014-08-01
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Series: | Cell Transplantation |
Online Access: | https://doi.org/10.3727/096368913X666403 |
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doaj-9855b86f5034458697f43daf0310d7782020-11-25T03:39:32ZengSAGE PublishingCell Transplantation0963-68971555-38922014-08-012310.3727/096368913X666403EpCAM Induction Functionally Links to the Wnt-Enhanced Cell Proliferation in Human KeratinocytesChing-I Shen0Hsiu-Chin Lee1Ying-Hsien Kao2Chieh-Shan Wu3Po-Hung Chen4Shinn-Zong Lin5Ping-Shan Lai6Hong-Lin Su7Department of Chemistry, Agricultural Biotechnology Center, National Chung-Hsing University, TaiwanDepartment of Life Sciences, Agricultural Biotechnology Center, National Chung-Hsing University, TaiwanDepartment of Medical Research, E-DA Hospital, I-Shou University, TaiwanDepartment of Dermatology, Kaohsiung Veterans General Hospital, TaiwanChen Po-Hung Dermatologic Clinic, Kaohsiung, TaiwanDepartment of Immunology, China Medical University, TaiwanDepartment of Chemistry, Agricultural Biotechnology Center, National Chung-Hsing University, TaiwanDepartment of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung, TaiwanAccelerating proliferation of primary keratinocytes benefits skin autografts for severely burned patients. Wnt signal, a conserved pathway controlling cell cycle and morphogenesis in embryo, also involves in cell proliferation and tumorigenesis in adult tissues. Here the effects of Wnt signal on the growth of human interfollicular keratinocytes were investigated. We demonstrated that recombinant Wnt3a significantly promoted the growth of primary keratinocytes at a low cell density. A well-characterized GSK-3b inhibitor, BIO, activated the Wnt signals and also enhanced the colony formation of keratinocytes dose dependently. Gene expression profile of the BIO-treated keratinocytes revealed the linkage of BIO with cell mitosis and indicated that epithelial cell adhesion molecule (EpCAM), a Wnt target gene, was significantly upregulated. Compared to the sorted EpCAM - keratinocytes, the EpCAM + cells showed a higher proliferation rate and efficacy of colony formation. Inhibiting the EpCAM expression by shRNA attenuated the proliferation effect of BIO and the growth advantage of the EpCAM + keratinocytes. These evidences emphasize the positive roles of canonical Wnt and EpCAM on the regulation of cell growth and self-renewal of human keratinocytes.https://doi.org/10.3727/096368913X666403 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ching-I Shen Hsiu-Chin Lee Ying-Hsien Kao Chieh-Shan Wu Po-Hung Chen Shinn-Zong Lin Ping-Shan Lai Hong-Lin Su |
spellingShingle |
Ching-I Shen Hsiu-Chin Lee Ying-Hsien Kao Chieh-Shan Wu Po-Hung Chen Shinn-Zong Lin Ping-Shan Lai Hong-Lin Su EpCAM Induction Functionally Links to the Wnt-Enhanced Cell Proliferation in Human Keratinocytes Cell Transplantation |
author_facet |
Ching-I Shen Hsiu-Chin Lee Ying-Hsien Kao Chieh-Shan Wu Po-Hung Chen Shinn-Zong Lin Ping-Shan Lai Hong-Lin Su |
author_sort |
Ching-I Shen |
title |
EpCAM Induction Functionally Links to the Wnt-Enhanced Cell Proliferation in Human Keratinocytes |
title_short |
EpCAM Induction Functionally Links to the Wnt-Enhanced Cell Proliferation in Human Keratinocytes |
title_full |
EpCAM Induction Functionally Links to the Wnt-Enhanced Cell Proliferation in Human Keratinocytes |
title_fullStr |
EpCAM Induction Functionally Links to the Wnt-Enhanced Cell Proliferation in Human Keratinocytes |
title_full_unstemmed |
EpCAM Induction Functionally Links to the Wnt-Enhanced Cell Proliferation in Human Keratinocytes |
title_sort |
epcam induction functionally links to the wnt-enhanced cell proliferation in human keratinocytes |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2014-08-01 |
description |
Accelerating proliferation of primary keratinocytes benefits skin autografts for severely burned patients. Wnt signal, a conserved pathway controlling cell cycle and morphogenesis in embryo, also involves in cell proliferation and tumorigenesis in adult tissues. Here the effects of Wnt signal on the growth of human interfollicular keratinocytes were investigated. We demonstrated that recombinant Wnt3a significantly promoted the growth of primary keratinocytes at a low cell density. A well-characterized GSK-3b inhibitor, BIO, activated the Wnt signals and also enhanced the colony formation of keratinocytes dose dependently. Gene expression profile of the BIO-treated keratinocytes revealed the linkage of BIO with cell mitosis and indicated that epithelial cell adhesion molecule (EpCAM), a Wnt target gene, was significantly upregulated. Compared to the sorted EpCAM - keratinocytes, the EpCAM + cells showed a higher proliferation rate and efficacy of colony formation. Inhibiting the EpCAM expression by shRNA attenuated the proliferation effect of BIO and the growth advantage of the EpCAM + keratinocytes. These evidences emphasize the positive roles of canonical Wnt and EpCAM on the regulation of cell growth and self-renewal of human keratinocytes. |
url |
https://doi.org/10.3727/096368913X666403 |
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