The detection and subsequent volume optimization of biological nanocrystals
Identifying and then optimizing initial crystallization conditions is a prerequisite for macromolecular structure determination by crystallography. Improved technologies enable data collection on crystals that are difficult if not impossible to detect using visible imag...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AIP Publishing LLC and ACA
2015-07-01
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| Series: | Structural Dynamics |
| Online Access: | http://dx.doi.org/10.1063/1.4921199 |
| Summary: | Identifying and then optimizing initial crystallization conditions is a prerequisite for
macromolecular structure determination by crystallography. Improved technologies
enable data collection on crystals that are difficult if not impossible to detect using
visible imaging. The application of second-order nonlinear imaging of chiral crystals and
ultraviolet two-photon excited fluorescence detection is shown to be applicable in a
high-throughput manner to rapidly verify the presence of nanocrystals in crystallization
screening conditions. It is noted that the nanocrystals are rarely seen without also producing
microcrystals from other chemical conditions. A crystal volume optimization method is
described and associated with a phase diagram for crystallization. |
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| ISSN: | 2329-7778 |