The detection and subsequent volume optimization of biological nanocrystals

Identifying and then optimizing initial crystallization conditions is a prerequisite for macromolecular structure determination by crystallography. Improved technologies enable data collection on crystals that are difficult if not impossible to detect using visible imag...

Full description

Bibliographic Details
Main Authors:	Joseph R. Luft, Jennifer R. Wolfley, Eleanor Cook Franks, Angela M. Lauricella, Ellen J. Gualtieri, Edward H. Snell, Rong Xiao, John K. Everett, Gaetano T. Montelione
Format:	Article
Language:	English
Published:	AIP Publishing LLC and ACA 2015-07-01
Series:	Structural Dynamics
Online Access:	http://dx.doi.org/10.1063/1.4921199

id	doaj-9866f852b1d941f08eb6c88c4e015247
record_format	Article
spelling	doaj-9866f852b1d941f08eb6c88c4e0152472020-11-25T02:26:03ZengAIP Publishing LLC and ACAStructural Dynamics2329-77782015-07-0124041710041710-1710.1063/1.4921199010591SDYThe detection and subsequent volume optimization of biological nanocrystalsJoseph R. Luft0Jennifer R. Wolfley1Eleanor Cook Franks2Angela M. Lauricella3Ellen J. Gualtieri4Edward H. Snell5Rong Xiao6John K. Everett7Gaetano T. Montelione8 Hauptman-Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, New York 14203, USA Hauptman-Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, New York 14203, USA Hauptman-Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, New York 14203, USA Hauptman-Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, New York 14203, USA Formulatrix, Inc., 10 DeAngelo Drive, Bedford, Massachusetts 01730, USA Hauptman-Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, New York 14203, USA Department of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, and Northeast Structural Genomics Consortium, Rutgers, The State University of New Jersey, 679 Hoes Lane, Piscataway, New Jersey 08854-8021, USA Department of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, and Northeast Structural Genomics Consortium, Rutgers, The State University of New Jersey, 679 Hoes Lane, Piscataway, New Jersey 08854-8021, USA Department of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, and Northeast Structural Genomics Consortium, Rutgers, The State University of New Jersey, 679 Hoes Lane, Piscataway, New Jersey 08854-8021, USAIdentifying and then optimizing initial crystallization conditions is a prerequisite for macromolecular structure determination by crystallography. Improved technologies enable data collection on crystals that are difficult if not impossible to detect using visible imaging. The application of second-order nonlinear imaging of chiral crystals and ultraviolet two-photon excited fluorescence detection is shown to be applicable in a high-throughput manner to rapidly verify the presence of nanocrystals in crystallization screening conditions. It is noted that the nanocrystals are rarely seen without also producing microcrystals from other chemical conditions. A crystal volume optimization method is described and associated with a phase diagram for crystallization.http://dx.doi.org/10.1063/1.4921199
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Joseph R. Luft Jennifer R. Wolfley Eleanor Cook Franks Angela M. Lauricella Ellen J. Gualtieri Edward H. Snell Rong Xiao John K. Everett Gaetano T. Montelione
spellingShingle	Joseph R. Luft Jennifer R. Wolfley Eleanor Cook Franks Angela M. Lauricella Ellen J. Gualtieri Edward H. Snell Rong Xiao John K. Everett Gaetano T. Montelione The detection and subsequent volume optimization of biological nanocrystals Structural Dynamics
author_facet	Joseph R. Luft Jennifer R. Wolfley Eleanor Cook Franks Angela M. Lauricella Ellen J. Gualtieri Edward H. Snell Rong Xiao John K. Everett Gaetano T. Montelione
author_sort	Joseph R. Luft
title	The detection and subsequent volume optimization of biological nanocrystals
title_short	The detection and subsequent volume optimization of biological nanocrystals
title_full	The detection and subsequent volume optimization of biological nanocrystals
title_fullStr	The detection and subsequent volume optimization of biological nanocrystals
title_full_unstemmed	The detection and subsequent volume optimization of biological nanocrystals
title_sort	detection and subsequent volume optimization of biological nanocrystals
publisher	AIP Publishing LLC and ACA
series	Structural Dynamics
issn	2329-7778
publishDate	2015-07-01
description	Identifying and then optimizing initial crystallization conditions is a prerequisite for macromolecular structure determination by crystallography. Improved technologies enable data collection on crystals that are difficult if not impossible to detect using visible imaging. The application of second-order nonlinear imaging of chiral crystals and ultraviolet two-photon excited fluorescence detection is shown to be applicable in a high-throughput manner to rapidly verify the presence of nanocrystals in crystallization screening conditions. It is noted that the nanocrystals are rarely seen without also producing microcrystals from other chemical conditions. A crystal volume optimization method is described and associated with a phase diagram for crystallization.
url	http://dx.doi.org/10.1063/1.4921199
work_keys_str_mv	AT josephrluft thedetectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT jenniferrwolfley thedetectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT eleanorcookfranks thedetectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT angelamlauricella thedetectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT ellenjgualtieri thedetectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT edwardhsnell thedetectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT rongxiao thedetectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT johnkeverett thedetectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT gaetanotmontelione thedetectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT josephrluft detectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT jenniferrwolfley detectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT eleanorcookfranks detectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT angelamlauricella detectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT ellenjgualtieri detectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT edwardhsnell detectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT rongxiao detectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT johnkeverett detectionandsubsequentvolumeoptimizationofbiologicalnanocrystals AT gaetanotmontelione detectionandsubsequentvolumeoptimizationofbiologicalnanocrystals
_version_	1724848721369235456

The detection and subsequent volume optimization of biological nanocrystals

Similar Items