A SIMPLE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF TETRACYCLINE AND DOXYCYCLINE IN PHARMACEUTICAL FORMULATIONS USING CHLORAMINE-T
A simple, rapid, accurate and inexpensive spectrophotometric method for the determination of tetracycline and doxycycline has been developed. The method is based on the reaction between these drugs and chloramine-T in alkaline medium producing red color products with absorbance maximum at the ë = 53...
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Universidade Estadual Paulista
2018-04-01
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doaj-9888b7a379a643acb0201448a4b14c032020-11-25T00:58:16ZengUniversidade Estadual PaulistaEclética Química1678-46182018-04-0135413914510.26850/1678-4618eqj.v35.4.2010.p139-145241A SIMPLE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF TETRACYCLINE AND DOXYCYCLINE IN PHARMACEUTICAL FORMULATIONS USING CHLORAMINE-TJosé Luiz Rufino0Flávio Cesar Bedatty Fernandes1Mayara S. Ruy2Helena Redigolo Pezza3Leonardo Pezza4São Paulo State University - UNESP, Institute of Chemistry of AraraquaraSão Paulo State University - UNESP, Institute of Chemistry of AraraquaraSão Paulo State University - UNESP, Institute of Chemistry of AraraquaraSão Paulo State University - UNESP, Institute of Chemistry of AraraquaraSão Paulo State University - UNESP, Institute of Chemistry of AraraquaraA simple, rapid, accurate and inexpensive spectrophotometric method for the determination of tetracycline and doxycycline has been developed. The method is based on the reaction between these drugs and chloramine-T in alkaline medium producing red color products with absorbance maximum at the ë = 535 and 525 nm for the tetracycline and doxycycline, respectively. The best conditions for the reactions have been found using multivariate method. Beer´s law is obeyed in a concentration ranges 1.03 x 10-5 to 3.61 x 10–4 mol L-1 and 1.75 x 10-5 to 3.48 x 10–4 mol L-1 for the tetracycline and doxycycline, respectively. The quantification limits were 5.63 x 10-6 mol L-1 and 7.12 x 10-7 mol L-1 for the tetracycline and doxycycline, respectively. The proposed method was successfully applied to the determination of these drugs in pharmaceutical formulations and the results obtained were in good agreement with those obtained by the comparative method at the 95% confidence level.https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/241 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
José Luiz Rufino Flávio Cesar Bedatty Fernandes Mayara S. Ruy Helena Redigolo Pezza Leonardo Pezza |
spellingShingle |
José Luiz Rufino Flávio Cesar Bedatty Fernandes Mayara S. Ruy Helena Redigolo Pezza Leonardo Pezza A SIMPLE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF TETRACYCLINE AND DOXYCYCLINE IN PHARMACEUTICAL FORMULATIONS USING CHLORAMINE-T Eclética Química |
author_facet |
José Luiz Rufino Flávio Cesar Bedatty Fernandes Mayara S. Ruy Helena Redigolo Pezza Leonardo Pezza |
author_sort |
José Luiz Rufino |
title |
A SIMPLE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF TETRACYCLINE AND DOXYCYCLINE IN PHARMACEUTICAL FORMULATIONS USING CHLORAMINE-T |
title_short |
A SIMPLE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF TETRACYCLINE AND DOXYCYCLINE IN PHARMACEUTICAL FORMULATIONS USING CHLORAMINE-T |
title_full |
A SIMPLE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF TETRACYCLINE AND DOXYCYCLINE IN PHARMACEUTICAL FORMULATIONS USING CHLORAMINE-T |
title_fullStr |
A SIMPLE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF TETRACYCLINE AND DOXYCYCLINE IN PHARMACEUTICAL FORMULATIONS USING CHLORAMINE-T |
title_full_unstemmed |
A SIMPLE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF TETRACYCLINE AND DOXYCYCLINE IN PHARMACEUTICAL FORMULATIONS USING CHLORAMINE-T |
title_sort |
simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-t |
publisher |
Universidade Estadual Paulista |
series |
Eclética Química |
issn |
1678-4618 |
publishDate |
2018-04-01 |
description |
A simple, rapid, accurate and inexpensive spectrophotometric method for the determination of tetracycline and doxycycline has been developed. The method is based on the reaction between these drugs and chloramine-T in alkaline medium producing red color products with absorbance maximum at the ë = 535 and 525 nm for the tetracycline and doxycycline, respectively. The best conditions for the reactions have been found using multivariate method. Beer´s law is obeyed in a concentration ranges 1.03 x 10-5 to 3.61 x 10–4 mol L-1 and 1.75 x 10-5 to 3.48 x 10–4 mol L-1 for the tetracycline and doxycycline, respectively. The quantification limits were 5.63 x 10-6 mol L-1 and 7.12 x 10-7 mol L-1 for the tetracycline and doxycycline, respectively. The proposed method was successfully applied to the determination of these drugs in pharmaceutical formulations and the results obtained were in good agreement with those obtained by the comparative method at the 95% confidence level. |
url |
https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/241 |
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