The Solanum lycopersicum WRKY3 Transcription Factor SlWRKY3 Is Involved in Salt Stress Tolerance in Tomato
Salinity threatens productivity of economically important crops such as tomato (Solanum lycopersicum L.). WRKY transcription factors appear, from a growing body of knowledge, as important regulators of abiotic stresses tolerance. Tomato SlWRKY3 is a nuclear protein binding to the consensus CGTTGACC/...
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2017-07-01
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doaj-98af73aa735c43c69f2c76c418e3279a2020-11-24T22:29:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-07-01810.3389/fpls.2017.01343283400The Solanum lycopersicum WRKY3 Transcription Factor SlWRKY3 Is Involved in Salt Stress Tolerance in TomatoImène Hichri0Yordan Muhovski1Eva Žižková2Petre I. Dobrev3Emna Gharbi4Jose M. Franco-Zorrilla5Irene Lopez-Vidriero6Roberto Solano7André Clippe8Abdelmounaim Errachid9Vaclav Motyka10Stanley Lutts11Groupe de Recherche en Physiologie Végétale, Earth and Life Institute - Agronomy, Université Catholique de LouvainLouvain-la-Neuve, BelgiumDépartement Sciences du Vivant, Centre Wallon de Recherches AgronomiquesGembloux, BelgiumInstitute of Experimental Botany, Academy of Sciences of the Czech RepublicPrague, CzechiaInstitute of Experimental Botany, Academy of Sciences of the Czech RepublicPrague, CzechiaGroupe de Recherche en Physiologie Végétale, Earth and Life Institute - Agronomy, Université Catholique de LouvainLouvain-la-Neuve, BelgiumDepartamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, Campus Universidad AutónomaMadrid, SpainDepartamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, Campus Universidad AutónomaMadrid, SpainDepartamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas, Campus Universidad AutónomaMadrid, SpainInstitut des Sciences de la Vie, Université Catholique de LouvainLouvain-la-Neuve, BelgiumInstitut des Sciences de la Vie, Université Catholique de LouvainLouvain-la-Neuve, BelgiumInstitute of Experimental Botany, Academy of Sciences of the Czech RepublicPrague, CzechiaGroupe de Recherche en Physiologie Végétale, Earth and Life Institute - Agronomy, Université Catholique de LouvainLouvain-la-Neuve, BelgiumSalinity threatens productivity of economically important crops such as tomato (Solanum lycopersicum L.). WRKY transcription factors appear, from a growing body of knowledge, as important regulators of abiotic stresses tolerance. Tomato SlWRKY3 is a nuclear protein binding to the consensus CGTTGACC/T W box. SlWRKY3 is preferentially expressed in aged organs, and is rapidly induced by NaCl, KCl, and drought. In addition, SlWRKY3 responds to salicylic acid, and 35S::SlWRKY3 tomatoes showed under salt treatment reduced contents of salicylic acid. In tomato, overexpression of SlWRKY3 impacted multiple aspects of salinity tolerance. Indeed, salinized (125 mM NaCl, 20 days) 35S::SlWRKY3 tomato plants displayed reduced oxidative stress and proline contents compared to WT. Physiological parameters related to plant growth (shoot and root biomass) and photosynthesis (stomatal conductance and chlorophyll a content) were retained in transgenic plants, together with lower Na+ contents in leaves, and higher accumulation of K+ and Ca2+. Microarray analysis confirmed that many stress-related genes were already up-regulated in transgenic tomatoes under optimal conditions of growth, including genes coding for antioxidant enzymes, ion and water transporters, or plant defense proteins. Together, these results indicate that SlWRKY3 is an important regulator of salinity tolerance in tomato.http://journal.frontiersin.org/article/10.3389/fpls.2017.01343/fullSolanum lycopersicumSlWRKY3transcription factorsalinity toleranceplant physiology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Imène Hichri Yordan Muhovski Eva Žižková Petre I. Dobrev Emna Gharbi Jose M. Franco-Zorrilla Irene Lopez-Vidriero Roberto Solano André Clippe Abdelmounaim Errachid Vaclav Motyka Stanley Lutts |
spellingShingle |
Imène Hichri Yordan Muhovski Eva Žižková Petre I. Dobrev Emna Gharbi Jose M. Franco-Zorrilla Irene Lopez-Vidriero Roberto Solano André Clippe Abdelmounaim Errachid Vaclav Motyka Stanley Lutts The Solanum lycopersicum WRKY3 Transcription Factor SlWRKY3 Is Involved in Salt Stress Tolerance in Tomato Frontiers in Plant Science Solanum lycopersicum SlWRKY3 transcription factor salinity tolerance plant physiology |
author_facet |
Imène Hichri Yordan Muhovski Eva Žižková Petre I. Dobrev Emna Gharbi Jose M. Franco-Zorrilla Irene Lopez-Vidriero Roberto Solano André Clippe Abdelmounaim Errachid Vaclav Motyka Stanley Lutts |
author_sort |
Imène Hichri |
title |
The Solanum lycopersicum WRKY3 Transcription Factor SlWRKY3 Is Involved in Salt Stress Tolerance in Tomato |
title_short |
The Solanum lycopersicum WRKY3 Transcription Factor SlWRKY3 Is Involved in Salt Stress Tolerance in Tomato |
title_full |
The Solanum lycopersicum WRKY3 Transcription Factor SlWRKY3 Is Involved in Salt Stress Tolerance in Tomato |
title_fullStr |
The Solanum lycopersicum WRKY3 Transcription Factor SlWRKY3 Is Involved in Salt Stress Tolerance in Tomato |
title_full_unstemmed |
The Solanum lycopersicum WRKY3 Transcription Factor SlWRKY3 Is Involved in Salt Stress Tolerance in Tomato |
title_sort |
solanum lycopersicum wrky3 transcription factor slwrky3 is involved in salt stress tolerance in tomato |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2017-07-01 |
description |
Salinity threatens productivity of economically important crops such as tomato (Solanum lycopersicum L.). WRKY transcription factors appear, from a growing body of knowledge, as important regulators of abiotic stresses tolerance. Tomato SlWRKY3 is a nuclear protein binding to the consensus CGTTGACC/T W box. SlWRKY3 is preferentially expressed in aged organs, and is rapidly induced by NaCl, KCl, and drought. In addition, SlWRKY3 responds to salicylic acid, and 35S::SlWRKY3 tomatoes showed under salt treatment reduced contents of salicylic acid. In tomato, overexpression of SlWRKY3 impacted multiple aspects of salinity tolerance. Indeed, salinized (125 mM NaCl, 20 days) 35S::SlWRKY3 tomato plants displayed reduced oxidative stress and proline contents compared to WT. Physiological parameters related to plant growth (shoot and root biomass) and photosynthesis (stomatal conductance and chlorophyll a content) were retained in transgenic plants, together with lower Na+ contents in leaves, and higher accumulation of K+ and Ca2+. Microarray analysis confirmed that many stress-related genes were already up-regulated in transgenic tomatoes under optimal conditions of growth, including genes coding for antioxidant enzymes, ion and water transporters, or plant defense proteins. Together, these results indicate that SlWRKY3 is an important regulator of salinity tolerance in tomato. |
topic |
Solanum lycopersicum SlWRKY3 transcription factor salinity tolerance plant physiology |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2017.01343/full |
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