Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells
The main vector of dengue in America is the mosquito Aedes aegypti, which is infected by dengue virus (DENV) through receptors of midgut epithelial cells. The envelope protein (E) of dengue virus binds to receptors present on the host cells through its domain III that has been primarily recognized t...
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doaj-98b718d6526947e3b3f0ef1516d2b8db2020-11-24T22:42:40ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/875958875958Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus CellsMaria de Lourdes Muñoz0Gustavo Limón-Camacho1Rosalinda Tovar2Alvaro Diaz-Badillo3Guillermo Mendoza-Hernández4William C. Black5Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 07360 Mexico, DF, MexicoDepartment of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 07360 Mexico, DF, MexicoDepartment of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 07360 Mexico, DF, MexicoDepartment of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 07360 Mexico, DF, MexicoDepartment of Biochemestry, Faculty of Medicine, Universidad Nacional Autonoma de México, Edificio de Investigación, 04510 Mexico, DF, MexicoDepartment of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-0015, USAThe main vector of dengue in America is the mosquito Aedes aegypti, which is infected by dengue virus (DENV) through receptors of midgut epithelial cells. The envelope protein (E) of dengue virus binds to receptors present on the host cells through its domain III that has been primarily recognized to bind cell receptors. In order to identify potential receptors, proteins from mosquito midgut tissue and C6/36 cells were purified by affinity using columns with the recombinant E protein domain III (rE-DIII) or DENV particles bound covalently to Sepharose 4B to compare and evaluate their performance to bind proteins including putative receptors from female mosquitoes of Ae. aegypti. To determine their identity mass spectrometric analysis of purified proteins separated by polyacrylamide gel electrophoresis was performed. Our results indicate that both viral particles and rE-DIII bound proteins with the same apparent molecular weights of 57 and 67 kDa. In addition, viral particles bound high molecular weight proteins. Purified proteins identified were enolase, beta-adrenergic receptor kinase (beta-ARK), translation elongation factor EF-1 alpha/Tu, and cadherin.http://dx.doi.org/10.1155/2013/875958 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria de Lourdes Muñoz Gustavo Limón-Camacho Rosalinda Tovar Alvaro Diaz-Badillo Guillermo Mendoza-Hernández William C. Black |
spellingShingle |
Maria de Lourdes Muñoz Gustavo Limón-Camacho Rosalinda Tovar Alvaro Diaz-Badillo Guillermo Mendoza-Hernández William C. Black Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells BioMed Research International |
author_facet |
Maria de Lourdes Muñoz Gustavo Limón-Camacho Rosalinda Tovar Alvaro Diaz-Badillo Guillermo Mendoza-Hernández William C. Black |
author_sort |
Maria de Lourdes Muñoz |
title |
Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells |
title_short |
Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells |
title_full |
Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells |
title_fullStr |
Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells |
title_full_unstemmed |
Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells |
title_sort |
proteomic identification of dengue virus binding proteins in aedes aegypti mosquitoes and aedes albopictus cells |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
The main vector of dengue in America is the mosquito Aedes aegypti, which is infected by dengue virus (DENV) through receptors of midgut epithelial cells. The envelope protein (E) of dengue virus binds to receptors present on the host cells through its domain III that has been primarily recognized to bind cell receptors. In order to identify potential receptors, proteins from mosquito midgut tissue and C6/36 cells were purified by affinity using columns with the recombinant E protein domain III (rE-DIII) or DENV particles bound covalently to Sepharose 4B to compare and evaluate their performance to bind proteins including putative receptors from female mosquitoes of Ae. aegypti. To determine their identity mass spectrometric analysis of purified proteins separated by polyacrylamide gel electrophoresis was performed. Our results indicate that both viral particles and rE-DIII bound proteins with the same apparent molecular weights of 57 and 67 kDa. In addition, viral particles bound high molecular weight proteins. Purified proteins identified were enolase, beta-adrenergic receptor kinase (beta-ARK), translation elongation factor EF-1 alpha/Tu, and cadherin. |
url |
http://dx.doi.org/10.1155/2013/875958 |
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