Analysis of Diffracted Intensities from Finite Protein Crystals with Incomplete Unit Cells
Developments in experimental techniques in micro electron diffraction and serial X-ray crystallography provide the opportunity to collect diffraction data from protein nanocrystals. Incomplete unit cells on the surfaces of protein crystals can affect the distribution of diffracted intensities for cr...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2017-07-01
|
Series: | Crystals |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4352/7/7/220 |
id |
doaj-98c8a05c990b4b7b8075c2d6ee713f33 |
---|---|
record_format |
Article |
spelling |
doaj-98c8a05c990b4b7b8075c2d6ee713f332020-11-24T22:16:36ZengMDPI AGCrystals2073-43522017-07-017722010.3390/cryst7070220cryst7070220Analysis of Diffracted Intensities from Finite Protein Crystals with Incomplete Unit CellsSophie R. Williams0Ruben A. Dilanian1Harry M. Quiney2Andrew V. Martin3ARC Centre of Excellence in Advanced Molecular Imaging, School of Physics, University of Melbourne, Parkville, Victoria 3010, AustraliaSchool of Physics, University of Melbourne, Parkville, Victoria 3010, AustraliaARC Centre of Excellence in Advanced Molecular Imaging, School of Physics, University of Melbourne, Parkville, Victoria 3010, AustraliaARC Centre of Excellence in Advanced Molecular Imaging, School of Physics, University of Melbourne, Parkville, Victoria 3010, AustraliaDevelopments in experimental techniques in micro electron diffraction and serial X-ray crystallography provide the opportunity to collect diffraction data from protein nanocrystals. Incomplete unit cells on the surfaces of protein crystals can affect the distribution of diffracted intensities for crystals with very high surface-to-volume ratios. The extraction of structure factors from diffraction data for such finite protein crystals sizes is considered here. A theoretical model for the continuous diffracted intensity distribution for data merged from finite crystals with two symmetry-related sub-units of the conventional unit cell is presented. This is used to extend a whole-pattern fitting technique to account for incomplete unit cells in the extraction of structure factor amplitudes. The accuracy of structure factor amplitudes found from this whole-pattern fitting technique and from an integration approach are evaluated.https://www.mdpi.com/2073-4352/7/7/220finite protein crystalsincomplete unit cellsstructure factor amplitudesmicro electron diffractionserial X-ray crystallographyXFELswhole-pattern fitting |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sophie R. Williams Ruben A. Dilanian Harry M. Quiney Andrew V. Martin |
spellingShingle |
Sophie R. Williams Ruben A. Dilanian Harry M. Quiney Andrew V. Martin Analysis of Diffracted Intensities from Finite Protein Crystals with Incomplete Unit Cells Crystals finite protein crystals incomplete unit cells structure factor amplitudes micro electron diffraction serial X-ray crystallography XFELs whole-pattern fitting |
author_facet |
Sophie R. Williams Ruben A. Dilanian Harry M. Quiney Andrew V. Martin |
author_sort |
Sophie R. Williams |
title |
Analysis of Diffracted Intensities from Finite Protein Crystals with Incomplete Unit Cells |
title_short |
Analysis of Diffracted Intensities from Finite Protein Crystals with Incomplete Unit Cells |
title_full |
Analysis of Diffracted Intensities from Finite Protein Crystals with Incomplete Unit Cells |
title_fullStr |
Analysis of Diffracted Intensities from Finite Protein Crystals with Incomplete Unit Cells |
title_full_unstemmed |
Analysis of Diffracted Intensities from Finite Protein Crystals with Incomplete Unit Cells |
title_sort |
analysis of diffracted intensities from finite protein crystals with incomplete unit cells |
publisher |
MDPI AG |
series |
Crystals |
issn |
2073-4352 |
publishDate |
2017-07-01 |
description |
Developments in experimental techniques in micro electron diffraction and serial X-ray crystallography provide the opportunity to collect diffraction data from protein nanocrystals. Incomplete unit cells on the surfaces of protein crystals can affect the distribution of diffracted intensities for crystals with very high surface-to-volume ratios. The extraction of structure factors from diffraction data for such finite protein crystals sizes is considered here. A theoretical model for the continuous diffracted intensity distribution for data merged from finite crystals with two symmetry-related sub-units of the conventional unit cell is presented. This is used to extend a whole-pattern fitting technique to account for incomplete unit cells in the extraction of structure factor amplitudes. The accuracy of structure factor amplitudes found from this whole-pattern fitting technique and from an integration approach are evaluated. |
topic |
finite protein crystals incomplete unit cells structure factor amplitudes micro electron diffraction serial X-ray crystallography XFELs whole-pattern fitting |
url |
https://www.mdpi.com/2073-4352/7/7/220 |
work_keys_str_mv |
AT sophierwilliams analysisofdiffractedintensitiesfromfiniteproteincrystalswithincompleteunitcells AT rubenadilanian analysisofdiffractedintensitiesfromfiniteproteincrystalswithincompleteunitcells AT harrymquiney analysisofdiffractedintensitiesfromfiniteproteincrystalswithincompleteunitcells AT andrewvmartin analysisofdiffractedintensitiesfromfiniteproteincrystalswithincompleteunitcells |
_version_ |
1725788890237763584 |