Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1
Thermococcus onnurineus NA1 is an anaerobic archaeon usually found in a deep-sea hydrothermal vent area, which can use elemental sulfur (S0) as a terminal electron acceptor for energy. Sulfur, essential to many biomolecules such as sulfur-containing amino acids and cofactors including iron-sulfur cl...
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doaj-98ea6eb2995146d1a65b001cbc9f01652021-07-02T02:16:55ZengHindawi LimitedArchaea1472-36461472-36542017-01-01201710.1155/2017/53952935395293Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1Thien-Hoang Ho0Kim-Hung Huynh1Diem Quynh Nguyen2Hyunjae Park3Kyoungho Jung4Bookyo Sur5Yeh-Jin Ahn6Sun-Shin Cha7Lin-Woo Kang8Department of Biological Sciences, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Biological Sciences, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Biological Sciences, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Biological Sciences, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Biological Sciences, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Biological Sciences, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of KoreaDepartment of Life Science, Sangmyung University, 7 Hongji-dong, Jongno-gu, Seoul 03016, Republic of KoreaDepartment of Chemistry and Nano Science, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 03760, Republic of KoreaDepartment of Biological Sciences, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of KoreaThermococcus onnurineus NA1 is an anaerobic archaeon usually found in a deep-sea hydrothermal vent area, which can use elemental sulfur (S0) as a terminal electron acceptor for energy. Sulfur, essential to many biomolecules such as sulfur-containing amino acids and cofactors including iron-sulfur cluster, is usually mobilized from cysteine by the pyridoxal 5′-phosphate- (PLP-) dependent enzyme of cysteine desulfurase (CDS). We determined the crystal structures of CDS from Thermococcus onnurineus NA1 (ToCDS), which include native internal aldimine (NAT), gem-diamine (GD) with alanine, internal aldimine structure with existing alanine (IAA), and internal aldimine with persulfide-bound Cys356 (PSF) structures. The catalytic intermediate structures showed the dihedral angle rotation of Schiff-base linkage relative to the PLP pyridine ring. The ToCDS structures were compared with bacterial CDS structures, which will help us to understand the role and catalytic mechanism of ToCDS in the archaeon Thermococcus onnurineus NA1.http://dx.doi.org/10.1155/2017/5395293 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thien-Hoang Ho Kim-Hung Huynh Diem Quynh Nguyen Hyunjae Park Kyoungho Jung Bookyo Sur Yeh-Jin Ahn Sun-Shin Cha Lin-Woo Kang |
spellingShingle |
Thien-Hoang Ho Kim-Hung Huynh Diem Quynh Nguyen Hyunjae Park Kyoungho Jung Bookyo Sur Yeh-Jin Ahn Sun-Shin Cha Lin-Woo Kang Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1 Archaea |
author_facet |
Thien-Hoang Ho Kim-Hung Huynh Diem Quynh Nguyen Hyunjae Park Kyoungho Jung Bookyo Sur Yeh-Jin Ahn Sun-Shin Cha Lin-Woo Kang |
author_sort |
Thien-Hoang Ho |
title |
Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1 |
title_short |
Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1 |
title_full |
Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1 |
title_fullStr |
Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1 |
title_full_unstemmed |
Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1 |
title_sort |
catalytic intermediate crystal structures of cysteine desulfurase from the archaeon thermococcus onnurineus na1 |
publisher |
Hindawi Limited |
series |
Archaea |
issn |
1472-3646 1472-3654 |
publishDate |
2017-01-01 |
description |
Thermococcus onnurineus NA1 is an anaerobic archaeon usually found in a deep-sea hydrothermal vent area, which can use elemental sulfur (S0) as a terminal electron acceptor for energy. Sulfur, essential to many biomolecules such as sulfur-containing amino acids and cofactors including iron-sulfur cluster, is usually mobilized from cysteine by the pyridoxal 5′-phosphate- (PLP-) dependent enzyme of cysteine desulfurase (CDS). We determined the crystal structures of CDS from Thermococcus onnurineus NA1 (ToCDS), which include native internal aldimine (NAT), gem-diamine (GD) with alanine, internal aldimine structure with existing alanine (IAA), and internal aldimine with persulfide-bound Cys356 (PSF) structures. The catalytic intermediate structures showed the dihedral angle rotation of Schiff-base linkage relative to the PLP pyridine ring. The ToCDS structures were compared with bacterial CDS structures, which will help us to understand the role and catalytic mechanism of ToCDS in the archaeon Thermococcus onnurineus NA1. |
url |
http://dx.doi.org/10.1155/2017/5395293 |
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