Root System Architecture Plasticity of Bread Wheat in Response to Oxidative Burst under Extended Osmotic Stress
There is a demand for an increase in crop production because of the growing population, but water shortage hinders the expansion of wheat cultivation, one of the most important crops worldwide. Polyethylene glycol (PEG) was used to mimic drought stress due to its high osmotic potentials generated in...
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doaj-98f54139b35b461e8b59f870c717d02d2021-05-31T23:26:55ZengMDPI AGPlants2223-77472021-05-011093993910.3390/plants10050939Root System Architecture Plasticity of Bread Wheat in Response to Oxidative Burst under Extended Osmotic StressOmar Azab0Abdullah Al-Doss1Thobayet Alshahrani2Salah El-Hendawy3Adel M. Zakri4Ahmed M. Abd-ElGawad5Plant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaThere is a demand for an increase in crop production because of the growing population, but water shortage hinders the expansion of wheat cultivation, one of the most important crops worldwide. Polyethylene glycol (PEG) was used to mimic drought stress due to its high osmotic potentials generated in plants subjected to it. This study aimed to determine the root system architecture (RSA) plasticity of eight bread wheat genotypes under osmotic stress in relation to the oxidative status and mitochondrial membrane potential of their root tips. Osmotic stress application resulted in differences in the RSA between the eight genotypes, where genotypes were divided into adapted genotypes that have non-significant decreased values in lateral roots number (LRN) and total root length (TRL), while non-adapted genotypes have a significant decrease in LRN, TRL, root volume (RV), and root surface area (SA). Accumulation of intracellular ROS formation in root tips and elongation zone was observed in the non-adapted genotypes due to PEG-induced oxidative stress. Mitochondrial membrane potential (∆Ψm) was measured for both stress and non-stress treatments in the eight genotypes as a biomarker for programmed cell death as a result of induced osmotic stress, in correlation with RSA traits. PEG treatment increased scavenging capacity of the genotypes from 1.4-fold in the sensitive genotype Gemmiza 7 to 14.3-fold in the adapted genotype Sakha 94. The adapted genotypes showed greater root trait values, ∆Ψm plasticity correlated with high scavenging capacity, and less ROS accumulation in the root tissue, while the non-adapted genotypes showed little scavenging capacity in both treatments, accompanied by mitochondrial membrane permeability, suggesting mitochondrial dysfunction as a result of oxidative stress.https://www.mdpi.com/2223-7747/10/5/939oxidative stressroot system architecturedichlorofluorescin diacetatemitochondrial membrane stability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Omar Azab Abdullah Al-Doss Thobayet Alshahrani Salah El-Hendawy Adel M. Zakri Ahmed M. Abd-ElGawad |
spellingShingle |
Omar Azab Abdullah Al-Doss Thobayet Alshahrani Salah El-Hendawy Adel M. Zakri Ahmed M. Abd-ElGawad Root System Architecture Plasticity of Bread Wheat in Response to Oxidative Burst under Extended Osmotic Stress Plants oxidative stress root system architecture dichlorofluorescin diacetate mitochondrial membrane stability |
author_facet |
Omar Azab Abdullah Al-Doss Thobayet Alshahrani Salah El-Hendawy Adel M. Zakri Ahmed M. Abd-ElGawad |
author_sort |
Omar Azab |
title |
Root System Architecture Plasticity of Bread Wheat in Response to Oxidative Burst under Extended Osmotic Stress |
title_short |
Root System Architecture Plasticity of Bread Wheat in Response to Oxidative Burst under Extended Osmotic Stress |
title_full |
Root System Architecture Plasticity of Bread Wheat in Response to Oxidative Burst under Extended Osmotic Stress |
title_fullStr |
Root System Architecture Plasticity of Bread Wheat in Response to Oxidative Burst under Extended Osmotic Stress |
title_full_unstemmed |
Root System Architecture Plasticity of Bread Wheat in Response to Oxidative Burst under Extended Osmotic Stress |
title_sort |
root system architecture plasticity of bread wheat in response to oxidative burst under extended osmotic stress |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2021-05-01 |
description |
There is a demand for an increase in crop production because of the growing population, but water shortage hinders the expansion of wheat cultivation, one of the most important crops worldwide. Polyethylene glycol (PEG) was used to mimic drought stress due to its high osmotic potentials generated in plants subjected to it. This study aimed to determine the root system architecture (RSA) plasticity of eight bread wheat genotypes under osmotic stress in relation to the oxidative status and mitochondrial membrane potential of their root tips. Osmotic stress application resulted in differences in the RSA between the eight genotypes, where genotypes were divided into adapted genotypes that have non-significant decreased values in lateral roots number (LRN) and total root length (TRL), while non-adapted genotypes have a significant decrease in LRN, TRL, root volume (RV), and root surface area (SA). Accumulation of intracellular ROS formation in root tips and elongation zone was observed in the non-adapted genotypes due to PEG-induced oxidative stress. Mitochondrial membrane potential (∆Ψm) was measured for both stress and non-stress treatments in the eight genotypes as a biomarker for programmed cell death as a result of induced osmotic stress, in correlation with RSA traits. PEG treatment increased scavenging capacity of the genotypes from 1.4-fold in the sensitive genotype Gemmiza 7 to 14.3-fold in the adapted genotype Sakha 94. The adapted genotypes showed greater root trait values, ∆Ψm plasticity correlated with high scavenging capacity, and less ROS accumulation in the root tissue, while the non-adapted genotypes showed little scavenging capacity in both treatments, accompanied by mitochondrial membrane permeability, suggesting mitochondrial dysfunction as a result of oxidative stress. |
topic |
oxidative stress root system architecture dichlorofluorescin diacetate mitochondrial membrane stability |
url |
https://www.mdpi.com/2223-7747/10/5/939 |
work_keys_str_mv |
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