Lactobacillus plantarum C29 alleviates NF-κB activation and Th17/Treg imbalance in mice with TNBS-induced colitis
In this study, we examined whether Lactobacillus plantarum C29 could restore 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced T helper 17 (Th17)/regulatory T cells (Tregs) imbalance in mice. Treatment with C29 inhibited the differentiation of splenic T cells into Th17 cells and the expression of re...
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doaj-98f73420ccf641d49622f6588f2edc932020-11-25T02:21:16ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432018-01-0129157758910.1080/09540105.2017.14188411418841Lactobacillus plantarum C29 alleviates NF-κB activation and Th17/Treg imbalance in mice with TNBS-induced colitisJin-Ju Jeong0Hae-Ji Lee1Se-Eun Jang2Myung Joo Han3Dong-Hyun Kim4Kyung Hee UniversityKyung Hee UniversityKyung Hee UniversityKyung Hee UniversityKyung Hee UniversityIn this study, we examined whether Lactobacillus plantarum C29 could restore 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced T helper 17 (Th17)/regulatory T cells (Tregs) imbalance in mice. Treatment with C29 inhibited the differentiation of splenic T cells into Th17 cells and the expression of retinoic acid receptor-related orphan receptor gamma t (RORγt) and IL-17 in vitro, whereas promoting the differentiation into Tregs. Oral administration of Lactobacillus plantarum C29 in mice attenuated TNBS-induced colon shortening, myeloperoxidase (MPO) activity, inducible Nitric oxide (NO) synthase, and cyclooxygenase-2 expression, and activation of NF-κB in the colon of mice. C29 treatment downregulated TNF-α, IL-17, and IL-1β expression, while increasing IL-10 expression. C29 treatment suppressed TNBS-induced Th17 cell differentiation and reduced IL-17 and RORγt expression, while promoting the TNBS-suppressed Tregs differentiation and IL-10 and forkhead box P3 expression. These findings suggest that C29 can alleviate colitis by modulating NF-κB activation as well as Th17/Treg balance.http://dx.doi.org/10.1080/09540105.2017.1418841lactobacillus plantarumcolitist cell differentiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin-Ju Jeong Hae-Ji Lee Se-Eun Jang Myung Joo Han Dong-Hyun Kim |
spellingShingle |
Jin-Ju Jeong Hae-Ji Lee Se-Eun Jang Myung Joo Han Dong-Hyun Kim Lactobacillus plantarum C29 alleviates NF-κB activation and Th17/Treg imbalance in mice with TNBS-induced colitis Food and Agricultural Immunology lactobacillus plantarum colitis t cell differentiation |
author_facet |
Jin-Ju Jeong Hae-Ji Lee Se-Eun Jang Myung Joo Han Dong-Hyun Kim |
author_sort |
Jin-Ju Jeong |
title |
Lactobacillus plantarum C29 alleviates NF-κB activation and Th17/Treg imbalance in mice with TNBS-induced colitis |
title_short |
Lactobacillus plantarum C29 alleviates NF-κB activation and Th17/Treg imbalance in mice with TNBS-induced colitis |
title_full |
Lactobacillus plantarum C29 alleviates NF-κB activation and Th17/Treg imbalance in mice with TNBS-induced colitis |
title_fullStr |
Lactobacillus plantarum C29 alleviates NF-κB activation and Th17/Treg imbalance in mice with TNBS-induced colitis |
title_full_unstemmed |
Lactobacillus plantarum C29 alleviates NF-κB activation and Th17/Treg imbalance in mice with TNBS-induced colitis |
title_sort |
lactobacillus plantarum c29 alleviates nf-κb activation and th17/treg imbalance in mice with tnbs-induced colitis |
publisher |
Taylor & Francis Group |
series |
Food and Agricultural Immunology |
issn |
0954-0105 1465-3443 |
publishDate |
2018-01-01 |
description |
In this study, we examined whether Lactobacillus plantarum C29 could restore 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced T helper 17 (Th17)/regulatory T cells (Tregs) imbalance in mice. Treatment with C29 inhibited the differentiation of splenic T cells into Th17 cells and the expression of retinoic acid receptor-related orphan receptor gamma t (RORγt) and IL-17 in vitro, whereas promoting the differentiation into Tregs. Oral administration of Lactobacillus plantarum C29 in mice attenuated TNBS-induced colon shortening, myeloperoxidase (MPO) activity, inducible Nitric oxide (NO) synthase, and cyclooxygenase-2 expression, and activation of NF-κB in the colon of mice. C29 treatment downregulated TNF-α, IL-17, and IL-1β expression, while increasing IL-10 expression. C29 treatment suppressed TNBS-induced Th17 cell differentiation and reduced IL-17 and RORγt expression, while promoting the TNBS-suppressed Tregs differentiation and IL-10 and forkhead box P3 expression. These findings suggest that C29 can alleviate colitis by modulating NF-κB activation as well as Th17/Treg balance. |
topic |
lactobacillus plantarum colitis t cell differentiation |
url |
http://dx.doi.org/10.1080/09540105.2017.1418841 |
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