Value of serum cytokine biomarkers TNF-α, IL-4, sIL-2R and IFN-γ for use in monitoring bacterial load and anti-tuberculosis treatment progress
Tuberculosis (TB) patient serum cytokine levels may be predictive of anti-tuberculosis treatment progress. Here, serum levels of cytokines TNF-α, IL-4, sIL-2R and IFN-γ were measured then correlated to clinical TB manifestations, bacterial burden, chest imaging findings and clinical course. Study su...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2020-06-01
|
Series: | Cytokine: X |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2590153220300082 |
id |
doaj-99087a6303c74608a4781af9b1b59930 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wenjuan Nie Jun Wang Wei Jing Wenhui Shi Qingfeng Wang Xuerui Huang Baoyun Cai Qiping Ge Lihui Nie Xiqin Han Yadong Du Jing Wang Ru Guo Naihui Chu |
spellingShingle |
Wenjuan Nie Jun Wang Wei Jing Wenhui Shi Qingfeng Wang Xuerui Huang Baoyun Cai Qiping Ge Lihui Nie Xiqin Han Yadong Du Jing Wang Ru Guo Naihui Chu Value of serum cytokine biomarkers TNF-α, IL-4, sIL-2R and IFN-γ for use in monitoring bacterial load and anti-tuberculosis treatment progress Cytokine: X Serum cytokine Biomarker TNF-α IL-4 sIL-2R IFN-γ |
author_facet |
Wenjuan Nie Jun Wang Wei Jing Wenhui Shi Qingfeng Wang Xuerui Huang Baoyun Cai Qiping Ge Lihui Nie Xiqin Han Yadong Du Jing Wang Ru Guo Naihui Chu |
author_sort |
Wenjuan Nie |
title |
Value of serum cytokine biomarkers TNF-α, IL-4, sIL-2R and IFN-γ for use in monitoring bacterial load and anti-tuberculosis treatment progress |
title_short |
Value of serum cytokine biomarkers TNF-α, IL-4, sIL-2R and IFN-γ for use in monitoring bacterial load and anti-tuberculosis treatment progress |
title_full |
Value of serum cytokine biomarkers TNF-α, IL-4, sIL-2R and IFN-γ for use in monitoring bacterial load and anti-tuberculosis treatment progress |
title_fullStr |
Value of serum cytokine biomarkers TNF-α, IL-4, sIL-2R and IFN-γ for use in monitoring bacterial load and anti-tuberculosis treatment progress |
title_full_unstemmed |
Value of serum cytokine biomarkers TNF-α, IL-4, sIL-2R and IFN-γ for use in monitoring bacterial load and anti-tuberculosis treatment progress |
title_sort |
value of serum cytokine biomarkers tnf-α, il-4, sil-2r and ifn-γ for use in monitoring bacterial load and anti-tuberculosis treatment progress |
publisher |
Elsevier |
series |
Cytokine: X |
issn |
2590-1532 |
publishDate |
2020-06-01 |
description |
Tuberculosis (TB) patient serum cytokine levels may be predictive of anti-tuberculosis treatment progress. Here, serum levels of cytokines TNF-α, IL-4, sIL-2R and IFN-γ were measured then correlated to clinical TB manifestations, bacterial burden, chest imaging findings and clinical course. Study subjects included 67 newly diagnosed pulmonary TB (PTB) patients with active disease admitted to Beijing Chest Hospital for anti-TB chemotherapeutic treatment. Blood was drawn at 0 months (pre-treatment), 1–2 months (at any time between 1 and 2 month) and after 6 months completion of treatment and serum TNF-α, IL-4, sIL-2R and IFN-γ levels were measured in duplicate using enzyme-linked immunosorbent assays (ELISAs). Correlation analysis was conducted to evaluate sensitivity and specificity of cytokine levels as predictors of disease activity and treatment progress. The results indicated that the pre-treatment serum TNF-α level of the smear-negative group was lower than that of the smear 1+ group, while serum TNF-α after 6 months completion of treatment and IFN-γ levels at 1–2 months and after 6 months completion of treatment were significantly lower, respectively, than at 0 months (before treatment) (P < 0.05). Using a cut-off value of 845 pg/ml, serum TNF-α level was predictive of treatment progress, with a sensitivity of 51%, specificity of 60% and AUC of 0.594 (P = 0.013). Meanwhile, using a cut-off value of 393 pg/ml, serum IFN-γ provided superior monitoring efficacy, with a sensitivity of 60%, specificity of 64% and AUC of 0.651 (P = 0.017). In conclusion, both serum TNF-α and IFN-γ levels might be useful biomarkers for monitoring treatment progress. |
topic |
Serum cytokine Biomarker TNF-α IL-4 sIL-2R IFN-γ |
url |
http://www.sciencedirect.com/science/article/pii/S2590153220300082 |
work_keys_str_mv |
AT wenjuannie valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT junwang valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT weijing valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT wenhuishi valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT qingfengwang valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT xueruihuang valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT baoyuncai valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT qipingge valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT lihuinie valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT xiqinhan valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT yadongdu valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT jingwang valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT ruguo valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress AT naihuichu valueofserumcytokinebiomarkerstnfail4sil2randifngforuseinmonitoringbacterialloadandantituberculosistreatmentprogress |
_version_ |
1724888725051146240 |
spelling |
doaj-99087a6303c74608a4781af9b1b599302020-11-25T02:16:49ZengElsevierCytokine: X2590-15322020-06-0122100028Value of serum cytokine biomarkers TNF-α, IL-4, sIL-2R and IFN-γ for use in monitoring bacterial load and anti-tuberculosis treatment progressWenjuan Nie0Jun Wang1Wei Jing2Wenhui Shi3Qingfeng Wang4Xuerui Huang5Baoyun Cai6Qiping Ge7Lihui Nie8Xiqin Han9Yadong Du10Jing Wang11Ru Guo12Naihui Chu13Tuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaCorresponding author.; Tuberculosis Department, Beijing Chest Hospital Affiliated to Capital Medical University, No 9, Beiguan Street, Tongzhou District, Beijing 101149, PR ChinaTuberculosis (TB) patient serum cytokine levels may be predictive of anti-tuberculosis treatment progress. Here, serum levels of cytokines TNF-α, IL-4, sIL-2R and IFN-γ were measured then correlated to clinical TB manifestations, bacterial burden, chest imaging findings and clinical course. Study subjects included 67 newly diagnosed pulmonary TB (PTB) patients with active disease admitted to Beijing Chest Hospital for anti-TB chemotherapeutic treatment. Blood was drawn at 0 months (pre-treatment), 1–2 months (at any time between 1 and 2 month) and after 6 months completion of treatment and serum TNF-α, IL-4, sIL-2R and IFN-γ levels were measured in duplicate using enzyme-linked immunosorbent assays (ELISAs). Correlation analysis was conducted to evaluate sensitivity and specificity of cytokine levels as predictors of disease activity and treatment progress. The results indicated that the pre-treatment serum TNF-α level of the smear-negative group was lower than that of the smear 1+ group, while serum TNF-α after 6 months completion of treatment and IFN-γ levels at 1–2 months and after 6 months completion of treatment were significantly lower, respectively, than at 0 months (before treatment) (P < 0.05). Using a cut-off value of 845 pg/ml, serum TNF-α level was predictive of treatment progress, with a sensitivity of 51%, specificity of 60% and AUC of 0.594 (P = 0.013). Meanwhile, using a cut-off value of 393 pg/ml, serum IFN-γ provided superior monitoring efficacy, with a sensitivity of 60%, specificity of 64% and AUC of 0.651 (P = 0.017). In conclusion, both serum TNF-α and IFN-γ levels might be useful biomarkers for monitoring treatment progress.http://www.sciencedirect.com/science/article/pii/S2590153220300082Serum cytokineBiomarkerTNF-αIL-4sIL-2RIFN-γ |