Langmuir Monolayer Techniques for the Investigation of Model Bacterial Membranes and Antibiotic Biodegradation Mechanisms
The amounts of antibiotics of anthropogenic origin released and accumulated in the environment are known to have a negative impact on local communities of microorganisms, which leads to disturbances in the course of the biodegradation process and to growing antimicrobial resistance. This mini-review...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-09-01
|
Series: | Membranes |
Subjects: | |
Online Access: | https://www.mdpi.com/2077-0375/11/9/707 |
id |
doaj-990ce0b26dba40b9a4f077960f12d048 |
---|---|
record_format |
Article |
spelling |
doaj-990ce0b26dba40b9a4f077960f12d0482021-09-26T00:40:29ZengMDPI AGMembranes2077-03752021-09-011170770710.3390/membranes11090707Langmuir Monolayer Techniques for the Investigation of Model Bacterial Membranes and Antibiotic Biodegradation MechanismsMonika Rojewska0Wojciech Smułek1Ewa Kaczorek2Krystyna Prochaska3Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-965 Poznań, PolandInstitute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-965 Poznań, PolandInstitute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-965 Poznań, PolandInstitute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-965 Poznań, PolandThe amounts of antibiotics of anthropogenic origin released and accumulated in the environment are known to have a negative impact on local communities of microorganisms, which leads to disturbances in the course of the biodegradation process and to growing antimicrobial resistance. This mini-review covers up-to-date information regarding problems related to the omnipresence of antibiotics and their consequences for the world of bacteria. In order to understand the interaction of antibiotics with bacterial membranes, it is necessary to explain their interaction mechanism at the molecular level. Such molecular-level interactions can be probed with Langmuir monolayers representing the cell membrane. This mini-review describes monolayer experiments undertaken to investigate the impact of selected antibiotics on components of biomembranes, with particular emphasis on the role and content of individual phospholipids and lipopolysaccharides (LPS). It is shown that the Langmuir technique may provide information about the interactions between antibiotics and lipids at the mixed film surface (π–A isotherm) and about the penetration of the active substances into the phospholipid monolayer model membranes (relaxation of the monolayer). Effects induced by antibiotics on the bacterial membrane may be correlated with their bactericidal activity, which may be vital for the selection of appropriate bacterial consortia that would ensure a high degradation efficiency of pharmaceuticals in the environment.https://www.mdpi.com/2077-0375/11/9/707antibioticsLangmuir monolayerbacterial membraneLPSphospholipidsantibiotic resistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Monika Rojewska Wojciech Smułek Ewa Kaczorek Krystyna Prochaska |
spellingShingle |
Monika Rojewska Wojciech Smułek Ewa Kaczorek Krystyna Prochaska Langmuir Monolayer Techniques for the Investigation of Model Bacterial Membranes and Antibiotic Biodegradation Mechanisms Membranes antibiotics Langmuir monolayer bacterial membrane LPS phospholipids antibiotic resistance |
author_facet |
Monika Rojewska Wojciech Smułek Ewa Kaczorek Krystyna Prochaska |
author_sort |
Monika Rojewska |
title |
Langmuir Monolayer Techniques for the Investigation of Model Bacterial Membranes and Antibiotic Biodegradation Mechanisms |
title_short |
Langmuir Monolayer Techniques for the Investigation of Model Bacterial Membranes and Antibiotic Biodegradation Mechanisms |
title_full |
Langmuir Monolayer Techniques for the Investigation of Model Bacterial Membranes and Antibiotic Biodegradation Mechanisms |
title_fullStr |
Langmuir Monolayer Techniques for the Investigation of Model Bacterial Membranes and Antibiotic Biodegradation Mechanisms |
title_full_unstemmed |
Langmuir Monolayer Techniques for the Investigation of Model Bacterial Membranes and Antibiotic Biodegradation Mechanisms |
title_sort |
langmuir monolayer techniques for the investigation of model bacterial membranes and antibiotic biodegradation mechanisms |
publisher |
MDPI AG |
series |
Membranes |
issn |
2077-0375 |
publishDate |
2021-09-01 |
description |
The amounts of antibiotics of anthropogenic origin released and accumulated in the environment are known to have a negative impact on local communities of microorganisms, which leads to disturbances in the course of the biodegradation process and to growing antimicrobial resistance. This mini-review covers up-to-date information regarding problems related to the omnipresence of antibiotics and their consequences for the world of bacteria. In order to understand the interaction of antibiotics with bacterial membranes, it is necessary to explain their interaction mechanism at the molecular level. Such molecular-level interactions can be probed with Langmuir monolayers representing the cell membrane. This mini-review describes monolayer experiments undertaken to investigate the impact of selected antibiotics on components of biomembranes, with particular emphasis on the role and content of individual phospholipids and lipopolysaccharides (LPS). It is shown that the Langmuir technique may provide information about the interactions between antibiotics and lipids at the mixed film surface (π–A isotherm) and about the penetration of the active substances into the phospholipid monolayer model membranes (relaxation of the monolayer). Effects induced by antibiotics on the bacterial membrane may be correlated with their bactericidal activity, which may be vital for the selection of appropriate bacterial consortia that would ensure a high degradation efficiency of pharmaceuticals in the environment. |
topic |
antibiotics Langmuir monolayer bacterial membrane LPS phospholipids antibiotic resistance |
url |
https://www.mdpi.com/2077-0375/11/9/707 |
work_keys_str_mv |
AT monikarojewska langmuirmonolayertechniquesfortheinvestigationofmodelbacterialmembranesandantibioticbiodegradationmechanisms AT wojciechsmułek langmuirmonolayertechniquesfortheinvestigationofmodelbacterialmembranesandantibioticbiodegradationmechanisms AT ewakaczorek langmuirmonolayertechniquesfortheinvestigationofmodelbacterialmembranesandantibioticbiodegradationmechanisms AT krystynaprochaska langmuirmonolayertechniquesfortheinvestigationofmodelbacterialmembranesandantibioticbiodegradationmechanisms |
_version_ |
1716870102136127488 |