Undernourishment and Yersinia enterocolitica Enterocolitis Alter Intestinal Contractility in the Rabbit: Role of Smooth Muscle Contractile Protein Content
Previous studies have demonstrated that the longitudinal smooth muscle of rabbits infected with Yersinia enterocolitica and undernourished because of reduced food intake exhibit a significantly reduced ability to develop tension in response to carbachol compared with pair-fed animals, which are unin...
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1999-01-01
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Series: | Canadian Journal of Gastroenterology |
Online Access: | http://dx.doi.org/10.1155/1999/673635 |
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doaj-991ba8de1c0b413c897990de704fe5322020-11-24T20:58:59ZengHindawi LimitedCanadian Journal of Gastroenterology0835-79001999-01-0113431932410.1155/1999/673635Undernourishment and Yersinia enterocolitica Enterocolitis Alter Intestinal Contractility in the Rabbit: Role of Smooth Muscle Contractile Protein ContentBeth C Chin0Daimen TM Tan1R Brent Scott2Gastrointestinal Research Group and the Department of Pediatrics, University of Calgary, Calgary, Alberta, CanadaGastrointestinal Research Group and the Department of Pediatrics, University of Calgary, Calgary, Alberta, CanadaGastrointestinal Research Group and the Department of Pediatrics, University of Calgary, Calgary, Alberta, CanadaPrevious studies have demonstrated that the longitudinal smooth muscle of rabbits infected with Yersinia enterocolitica and undernourished because of reduced food intake exhibit a significantly reduced ability to develop tension in response to carbachol compared with pair-fed animals, which are uninfected but equivalently undernourished. To determine whether the alteration in smooth muscle contractility results from changes in cell number (hypo- or hyperplasia), or in contractile protein content or isoform distribution, New Zealand White rabbits (600 to 1000 g) were randomly assigned to one of three treatment groups: infected, pair-fed or control. Tissue contractility was measured, morphometric studies were performed and immunoassays were developed for the measurement of total actin, gamma-enteric and alpha-vascular isoactins, and myosin heavy chain. Consistent with what was found in previous reports, the contractility of longitudinal smooth muscle in response to carbachol was found to increase in pair-fed animals and to decrease in Y enterocolitica-infected animals. There was no significant change in the proportional thickness of the ileal longitudinal smooth muscle coat, and the number of cross-sectioned longitudinal smooth muscle cells/mm2 was not significantly different in infected, pair-fed or control tissues. Immunoassay indicated that the proportion of each specific contractile protein, relative to total protein content in the muscularis propria, was unaffected by Y enterocolitica infection or by pair-feeding. Thus, the alterations in intestinal longitudinal smooth muscle function observed after Y enterocolitica infection were concluded not to be associated with tissue hypo- or hyperplasia, or changes in the total content or isoform distribution of contractile proteins in the muscularis propria.http://dx.doi.org/10.1155/1999/673635 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Beth C Chin Daimen TM Tan R Brent Scott |
spellingShingle |
Beth C Chin Daimen TM Tan R Brent Scott Undernourishment and Yersinia enterocolitica Enterocolitis Alter Intestinal Contractility in the Rabbit: Role of Smooth Muscle Contractile Protein Content Canadian Journal of Gastroenterology |
author_facet |
Beth C Chin Daimen TM Tan R Brent Scott |
author_sort |
Beth C Chin |
title |
Undernourishment and Yersinia enterocolitica Enterocolitis Alter Intestinal Contractility in the Rabbit: Role of Smooth Muscle Contractile Protein Content |
title_short |
Undernourishment and Yersinia enterocolitica Enterocolitis Alter Intestinal Contractility in the Rabbit: Role of Smooth Muscle Contractile Protein Content |
title_full |
Undernourishment and Yersinia enterocolitica Enterocolitis Alter Intestinal Contractility in the Rabbit: Role of Smooth Muscle Contractile Protein Content |
title_fullStr |
Undernourishment and Yersinia enterocolitica Enterocolitis Alter Intestinal Contractility in the Rabbit: Role of Smooth Muscle Contractile Protein Content |
title_full_unstemmed |
Undernourishment and Yersinia enterocolitica Enterocolitis Alter Intestinal Contractility in the Rabbit: Role of Smooth Muscle Contractile Protein Content |
title_sort |
undernourishment and yersinia enterocolitica enterocolitis alter intestinal contractility in the rabbit: role of smooth muscle contractile protein content |
publisher |
Hindawi Limited |
series |
Canadian Journal of Gastroenterology |
issn |
0835-7900 |
publishDate |
1999-01-01 |
description |
Previous studies have demonstrated that the longitudinal smooth muscle of rabbits infected with Yersinia enterocolitica and undernourished because of reduced food intake exhibit a significantly reduced ability to develop tension in response to carbachol compared with pair-fed animals, which are uninfected but equivalently undernourished. To determine whether the alteration in smooth muscle contractility results from changes in cell number (hypo- or hyperplasia), or in contractile protein content or isoform distribution, New Zealand White rabbits (600 to 1000 g) were randomly assigned to one of three treatment groups: infected, pair-fed or control. Tissue contractility was measured, morphometric studies were performed and immunoassays were developed for the measurement of total actin, gamma-enteric and alpha-vascular isoactins, and myosin heavy chain. Consistent with what was found in previous reports, the contractility of longitudinal smooth muscle in response to carbachol was found to increase in pair-fed animals and to decrease in Y enterocolitica-infected animals. There was no significant change in the proportional thickness of the ileal longitudinal smooth muscle coat, and the number of cross-sectioned longitudinal smooth muscle cells/mm2 was not significantly different in infected, pair-fed or control tissues. Immunoassay indicated that the proportion of each specific contractile protein, relative to total protein content in the muscularis propria, was unaffected by Y enterocolitica infection or by pair-feeding. Thus, the alterations in intestinal longitudinal smooth muscle function observed after Y enterocolitica infection were concluded not to be associated with tissue hypo- or hyperplasia, or changes in the total content or isoform distribution of contractile proteins in the muscularis propria. |
url |
http://dx.doi.org/10.1155/1999/673635 |
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