Vancomycin Resistance Among Methicillin Resistant Staphylococcus aureus Isolates from Neonatal Sepsis Attending Intensive Care Unit in Shibin El-Kom Teaching Hospital, Egypt

• Main Authors: Mahmoud AbdEl-Mongy, Elham Taha Awad, Fatma Mosaed
• Format: Article
• Language: English
• Published: Journal of Pure and Applied Microbiology 2018-09-01
• Series: Journal of Pure and Applied Microbiology
• Subjects: mrsa; vrsa; nuc gene; antibiotic resistance
• Online Access: https://microbiologyjournal.org/vancomycin-resistance-among-methicillin-resistant-staphylococcus-aureus-isolates-from-neonatal-sepsis-attending-intensive-care-unit-in-shibin-el-kom-teaching-hospital-egypt/

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of Nosocomial, community acquired infections and neonatal sepsis. The Glycopeptide vancomycin was the drug of choice for treating infections. Aim: Identifying the vancomycin- resistance phenotypically and genotypically among the MRSA isolates from Shibin El-Kom teaching Hospital. Materials and Methods: All samples were collected from Neonatal intensive care unit (NICU) of Shibin El-Kom teaching hospital in Minoufiya, Egypt and identified by conventional methods. S aureus and MRSA were isolated and identified from different clinical samples using conventional methods confirmed by antibiogram of the isolates and mec A gene detection. vancomycin MIC and Vancomycin screening agar were determined following CLSI guidelines. Van A was amplified by PCR using standard primers. Out of the 200 neonates included in this study, 85% were positive growth and 15% were negative growth. Among them, 25% isolates were staphylococci, 42 isolates had nuc gene. Out of 42 S. aureus, 80.95% had mecA gene and 19.05% had not Mec A gene. The VRSA isolates had not van A gene. Conclusions: Vancomycin was still the most effective drug against S. aureus infection. All MRSA in Shibin El-Kom Teaching Hospital had not vanA gene.