Vancomycin Resistance Among Methicillin Resistant Staphylococcus aureus Isolates from Neonatal Sepsis Attending Intensive Care Unit in Shibin El-Kom Teaching Hospital, Egypt
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of Nosocomial, community acquired infections and neonatal sepsis. The Glycopeptide vancomycin was the drug of choice for treating infections. Aim: Identifying the vancomycin- resistance phenotypically and genotypically among the...
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doaj-99287594320e4d81a11573d36bb499622021-10-02T18:52:34ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2018-09-011231093110010.22207/JPAM.12.3.07Vancomycin Resistance Among Methicillin Resistant Staphylococcus aureus Isolates from Neonatal Sepsis Attending Intensive Care Unit in Shibin El-Kom Teaching Hospital, EgyptMahmoud AbdEl-Mongy0Elham Taha Awad1Fatma Mosaed2Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.Medical Microbiology and Immunology, Shibin El-kom Teaching Hospital, Monifiya, Egypt.Microbiology and Chemistry Department, Faculty of Science, Aswan University, Egypt.Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of Nosocomial, community acquired infections and neonatal sepsis. The Glycopeptide vancomycin was the drug of choice for treating infections. Aim: Identifying the vancomycin- resistance phenotypically and genotypically among the MRSA isolates from Shibin El-Kom teaching Hospital. Materials and Methods: All samples were collected from Neonatal intensive care unit (NICU) of Shibin El-Kom teaching hospital in Minoufiya, Egypt and identified by conventional methods. S aureus and MRSA were isolated and identified from different clinical samples using conventional methods confirmed by antibiogram of the isolates and mec A gene detection. vancomycin MIC and Vancomycin screening agar were determined following CLSI guidelines. Van A was amplified by PCR using standard primers. Out of the 200 neonates included in this study, 85% were positive growth and 15% were negative growth. Among them, 25% isolates were staphylococci, 42 isolates had nuc gene. Out of 42 S. aureus, 80.95% had mecA gene and 19.05% had not Mec A gene. The VRSA isolates had not van A gene. Conclusions: Vancomycin was still the most effective drug against S. aureus infection. All MRSA in Shibin El-Kom Teaching Hospital had not vanA gene.https://microbiologyjournal.org/vancomycin-resistance-among-methicillin-resistant-staphylococcus-aureus-isolates-from-neonatal-sepsis-attending-intensive-care-unit-in-shibin-el-kom-teaching-hospital-egypt/mrsavrsanuc geneantibiotic resistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mahmoud AbdEl-Mongy Elham Taha Awad Fatma Mosaed |
spellingShingle |
Mahmoud AbdEl-Mongy Elham Taha Awad Fatma Mosaed Vancomycin Resistance Among Methicillin Resistant Staphylococcus aureus Isolates from Neonatal Sepsis Attending Intensive Care Unit in Shibin El-Kom Teaching Hospital, Egypt Journal of Pure and Applied Microbiology mrsa vrsa nuc gene antibiotic resistance |
author_facet |
Mahmoud AbdEl-Mongy Elham Taha Awad Fatma Mosaed |
author_sort |
Mahmoud AbdEl-Mongy |
title |
Vancomycin Resistance Among Methicillin Resistant Staphylococcus aureus Isolates from Neonatal Sepsis Attending Intensive Care Unit in Shibin El-Kom Teaching Hospital, Egypt |
title_short |
Vancomycin Resistance Among Methicillin Resistant Staphylococcus aureus Isolates from Neonatal Sepsis Attending Intensive Care Unit in Shibin El-Kom Teaching Hospital, Egypt |
title_full |
Vancomycin Resistance Among Methicillin Resistant Staphylococcus aureus Isolates from Neonatal Sepsis Attending Intensive Care Unit in Shibin El-Kom Teaching Hospital, Egypt |
title_fullStr |
Vancomycin Resistance Among Methicillin Resistant Staphylococcus aureus Isolates from Neonatal Sepsis Attending Intensive Care Unit in Shibin El-Kom Teaching Hospital, Egypt |
title_full_unstemmed |
Vancomycin Resistance Among Methicillin Resistant Staphylococcus aureus Isolates from Neonatal Sepsis Attending Intensive Care Unit in Shibin El-Kom Teaching Hospital, Egypt |
title_sort |
vancomycin resistance among methicillin resistant staphylococcus aureus isolates from neonatal sepsis attending intensive care unit in shibin el-kom teaching hospital, egypt |
publisher |
Journal of Pure and Applied Microbiology |
series |
Journal of Pure and Applied Microbiology |
issn |
0973-7510 2581-690X |
publishDate |
2018-09-01 |
description |
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of Nosocomial,
community acquired infections and neonatal sepsis. The Glycopeptide vancomycin was the drug
of choice for treating infections. Aim: Identifying the vancomycin- resistance phenotypically and
genotypically among the MRSA isolates from Shibin El-Kom teaching Hospital. Materials and
Methods: All samples were collected from Neonatal intensive care unit (NICU) of Shibin El-Kom
teaching hospital in Minoufiya, Egypt and identified by conventional methods. S aureus and
MRSA were isolated and identified from different clinical samples using conventional methods
confirmed by antibiogram of the isolates and mec A gene detection. vancomycin MIC and
Vancomycin screening agar were determined following CLSI guidelines. Van A was amplified
by PCR using standard primers. Out of the 200 neonates included in this study, 85% were
positive growth and 15% were negative growth. Among them, 25% isolates were staphylococci,
42 isolates had nuc gene. Out of 42 S. aureus, 80.95% had mecA gene and 19.05% had not Mec
A gene. The VRSA isolates had not van A gene. Conclusions: Vancomycin was still the most
effective drug against S. aureus infection. All MRSA in Shibin El-Kom Teaching Hospital had
not vanA gene. |
topic |
mrsa vrsa nuc gene antibiotic resistance |
url |
https://microbiologyjournal.org/vancomycin-resistance-among-methicillin-resistant-staphylococcus-aureus-isolates-from-neonatal-sepsis-attending-intensive-care-unit-in-shibin-el-kom-teaching-hospital-egypt/ |
work_keys_str_mv |
AT mahmoudabdelmongy vancomycinresistanceamongmethicillinresistantstaphylococcusaureusisolatesfromneonatalsepsisattendingintensivecareunitinshibinelkomteachinghospitalegypt AT elhamtahaawad vancomycinresistanceamongmethicillinresistantstaphylococcusaureusisolatesfromneonatalsepsisattendingintensivecareunitinshibinelkomteachinghospitalegypt AT fatmamosaed vancomycinresistanceamongmethicillinresistantstaphylococcusaureusisolatesfromneonatalsepsisattendingintensivecareunitinshibinelkomteachinghospitalegypt |
_version_ |
1716848665355616256 |