Immunophenotyping of blood cells of experimental animals immunized with <i>Brucella abortus</i> thermoextracts

• Main Authors: S. V. Balakhonov, V. I. Dubrovina, V. V. Voitkova, K. M. Korytov, N. L. Barannikova, V. B. Nikolaev, T. T. Shkaruba
• Format: Article
• Language: Russian
• Published: Central Research Institute for Epidemiology 2019-09-01
• Series: Журнал микробиологии, эпидемиологии и иммунобиологии
• Subjects: <i>brucella abortus</i>; thermoextract; experimental animal; blood; flow cytometry
• Online Access: https://microbiol.elpub.ru/jour/article/view/426

Aim. To study the subpopulational structure of blood cells of the experimental animals immunized with thermoextracts (TE) of Brucella abortus in L- and S-form. Materials and methods. Total 100 certified («Vector», Novosibirsk) outbred mice were immunized with B. abortus I-206 TE in L- and S-form in 20 μg protein dose. After 1, 3, 7, 14 and 21 days of observation the phenotypes (CD45, CD3, CD4, CD8, CD19, CD69) of blood cells were detected. Results. General regularities were revealed after injection of the experimental preparations. So, B. abortus TE in L- and S-form caused the immune response that increased granulocyte number and expression of early activation marker CD69 by T- and B-lymphocytes of blood in early period of observation (1-3 days), decrease in general B-lymphocyte content in late periods of observation (7-21 days). Thus, mice received B. abortus ТE in L-form demonstrated authentically higher CD69 expression of blood lymphocyte subpopulations than mice received B. abortus ТE in S-form. Distinctions in formation of humoral immune response were revealed that probably was connected with alteration of Brucella chemical composition in the course of L-transformation. Conclusion. The investigation established that B. abortus TE in L- or S-form caused immunological reorganization in the experimental animal organisms. On the basis of the fin