Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo
Masamichi Fukuda1, Hiroshi Sasaki11Department of Ophthalmology, Kanazawa Medical University, Uchinada, JapanPurpose: We attempted to develop a device for measuring electrical corneal resistance (CR) using corneal contact lens electrodes to quantitatively evaluate corneal injury in vivo. In the prese...
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doaj-9944ce4db6ba440e9c680234c36412a32020-11-25T00:56:05ZengDove Medical PressClinical Ophthalmology1177-54671177-54832012-04-012012default585593Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivoFukuda MSasaki HMasamichi Fukuda1, Hiroshi Sasaki11Department of Ophthalmology, Kanazawa Medical University, Uchinada, JapanPurpose: We attempted to develop a device for measuring electrical corneal resistance (CR) using corneal contact lens electrodes to quantitatively evaluate corneal injury in vivo. In the present study, full-thickness detachment of the corneal epithelium was induced by n-heptanol, and the feasibility of the quantitative evaluation of this injury by corneal contact lens electrodes was evaluated in vivo.Methods: The central area of an albino rabbit's cornea was exposed to a filter paper pre-immersed in n-heptanol for 1 minute to induce injury of the corneal epithelium. After induction of injury, the electrical CR was measured and the percentage of CR (%CR) was calculated. Fluorescein solution (3 µL) was applied to the wound/affected area of the corneal epithelium for photography with a slit-lamp biomicroscope. The wound/affected area was measured using an image analysis system. The correlation between the %CR and the wound/affected area was analyzed.Results: As the size of the wound/affected area of the corneal epithelium increased, the %CR decreased after corneal epithelium detachment. Thus, a close correlation was found between the area of corneal epithelium detachment and the %CR.Conclusion: The corneal resistance device that we developed was capable of quantitatively evaluating n-heptanol-induced full-thickness injuries of the corneal epithelium.Keywords: eyes in vivo, corneal injury, corneal contact lens electrode corneal resistance device, n-heptanolhttp://www.dovepress.com/quantitative-evaluation-of-corneal-epithelial-injury-caused-by-n-hepta-a9707 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fukuda M Sasaki H |
spellingShingle |
Fukuda M Sasaki H Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo Clinical Ophthalmology |
author_facet |
Fukuda M Sasaki H |
author_sort |
Fukuda M |
title |
Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo |
title_short |
Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo |
title_full |
Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo |
title_fullStr |
Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo |
title_full_unstemmed |
Quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo |
title_sort |
quantitative evaluation of corneal epithelial injury caused by n-heptanol using a corneal resistance measuring device in vivo |
publisher |
Dove Medical Press |
series |
Clinical Ophthalmology |
issn |
1177-5467 1177-5483 |
publishDate |
2012-04-01 |
description |
Masamichi Fukuda1, Hiroshi Sasaki11Department of Ophthalmology, Kanazawa Medical University, Uchinada, JapanPurpose: We attempted to develop a device for measuring electrical corneal resistance (CR) using corneal contact lens electrodes to quantitatively evaluate corneal injury in vivo. In the present study, full-thickness detachment of the corneal epithelium was induced by n-heptanol, and the feasibility of the quantitative evaluation of this injury by corneal contact lens electrodes was evaluated in vivo.Methods: The central area of an albino rabbit's cornea was exposed to a filter paper pre-immersed in n-heptanol for 1 minute to induce injury of the corneal epithelium. After induction of injury, the electrical CR was measured and the percentage of CR (%CR) was calculated. Fluorescein solution (3 µL) was applied to the wound/affected area of the corneal epithelium for photography with a slit-lamp biomicroscope. The wound/affected area was measured using an image analysis system. The correlation between the %CR and the wound/affected area was analyzed.Results: As the size of the wound/affected area of the corneal epithelium increased, the %CR decreased after corneal epithelium detachment. Thus, a close correlation was found between the area of corneal epithelium detachment and the %CR.Conclusion: The corneal resistance device that we developed was capable of quantitatively evaluating n-heptanol-induced full-thickness injuries of the corneal epithelium.Keywords: eyes in vivo, corneal injury, corneal contact lens electrode corneal resistance device, n-heptanol |
url |
http://www.dovepress.com/quantitative-evaluation-of-corneal-epithelial-injury-caused-by-n-hepta-a9707 |
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