Arcobacter cryaerophilus Isolated From New Zealand Mussels Harbor a Putative Virulence Plasmid
A wide range of Arcobacter species have been described from shellfish in various countries but their presence has not been investigated in Australasia, in which shellfish are a popular delicacy. Since several arcobacters are considered to be emerging pathogens, we undertook a small study to evaluate...
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doaj-9963985cff424e008cd0649d94ba78dd2020-11-24T21:21:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-08-011010.3389/fmicb.2019.01802466008Arcobacter cryaerophilus Isolated From New Zealand Mussels Harbor a Putative Virulence PlasmidStephen L. W. On0Damien Althaus1William G. Miller2Darrell Lizamore3Samuel G. L. Wong4Anso J. Mathai5Venkata Chelikani6Glen P. Carter7Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New ZealandDepartment of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New ZealandProduce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, United StatesDepartment of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New ZealandDepartment of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New ZealandDepartment of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New ZealandDepartment of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New ZealandDoherty Applied Microbial Genomics, The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC, AustraliaA wide range of Arcobacter species have been described from shellfish in various countries but their presence has not been investigated in Australasia, in which shellfish are a popular delicacy. Since several arcobacters are considered to be emerging pathogens, we undertook a small study to evaluate their presence in several different shellfish, including greenshell mussels, oysters, and abalone (paua) in New Zealand. Arcobacter cryaerophilus, a species associated with human gastroenteritis, was the only species isolated, from greenshell mussels. Whole-genome sequencing revealed a range of genomic traits in these strains that were known or associated virulence factors. Furthermore, we describe the first putative virulence plasmid in Arcobacter, containing lytic, immunoavoidance, adhesion, antibiotic resistance, and gene transfer traits, among others. Complete genome sequence determination using a combination of long- and short-read genome sequencing strategies, was needed to identify the plasmid, clearly identifying its benefits. The potential for plasmids to disseminate virulence traits among Arcobacter and other species warrants further consideration by researchers interested in the risks to public health from these organisms.https://www.frontiersin.org/article/10.3389/fmicb.2019.01802/fullArcobacter cryaerophilusshellfishmusselpathogenvirulence plasmid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stephen L. W. On Damien Althaus William G. Miller Darrell Lizamore Samuel G. L. Wong Anso J. Mathai Venkata Chelikani Glen P. Carter |
spellingShingle |
Stephen L. W. On Damien Althaus William G. Miller Darrell Lizamore Samuel G. L. Wong Anso J. Mathai Venkata Chelikani Glen P. Carter Arcobacter cryaerophilus Isolated From New Zealand Mussels Harbor a Putative Virulence Plasmid Frontiers in Microbiology Arcobacter cryaerophilus shellfish mussel pathogen virulence plasmid |
author_facet |
Stephen L. W. On Damien Althaus William G. Miller Darrell Lizamore Samuel G. L. Wong Anso J. Mathai Venkata Chelikani Glen P. Carter |
author_sort |
Stephen L. W. On |
title |
Arcobacter cryaerophilus Isolated From New Zealand Mussels Harbor a Putative Virulence Plasmid |
title_short |
Arcobacter cryaerophilus Isolated From New Zealand Mussels Harbor a Putative Virulence Plasmid |
title_full |
Arcobacter cryaerophilus Isolated From New Zealand Mussels Harbor a Putative Virulence Plasmid |
title_fullStr |
Arcobacter cryaerophilus Isolated From New Zealand Mussels Harbor a Putative Virulence Plasmid |
title_full_unstemmed |
Arcobacter cryaerophilus Isolated From New Zealand Mussels Harbor a Putative Virulence Plasmid |
title_sort |
arcobacter cryaerophilus isolated from new zealand mussels harbor a putative virulence plasmid |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-08-01 |
description |
A wide range of Arcobacter species have been described from shellfish in various countries but their presence has not been investigated in Australasia, in which shellfish are a popular delicacy. Since several arcobacters are considered to be emerging pathogens, we undertook a small study to evaluate their presence in several different shellfish, including greenshell mussels, oysters, and abalone (paua) in New Zealand. Arcobacter cryaerophilus, a species associated with human gastroenteritis, was the only species isolated, from greenshell mussels. Whole-genome sequencing revealed a range of genomic traits in these strains that were known or associated virulence factors. Furthermore, we describe the first putative virulence plasmid in Arcobacter, containing lytic, immunoavoidance, adhesion, antibiotic resistance, and gene transfer traits, among others. Complete genome sequence determination using a combination of long- and short-read genome sequencing strategies, was needed to identify the plasmid, clearly identifying its benefits. The potential for plasmids to disseminate virulence traits among Arcobacter and other species warrants further consideration by researchers interested in the risks to public health from these organisms. |
topic |
Arcobacter cryaerophilus shellfish mussel pathogen virulence plasmid |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.01802/full |
work_keys_str_mv |
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