Cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved Plasmodium vivax isolates.
The development of a system for the continuous culture of Plasmodium vivax in vitro would benefit from the use of reticulocytes derived from differentiated hematopoietic stem cells (HCS). At present, the need to use both fresh reticulocytes and fresh P. vivax isolates represents a major obstacle tow...
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2012-01-01
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doaj-997cfda45e3d418d9ef3690c3325e3102020-11-25T01:29:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4079810.1371/journal.pone.0040798Cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved Plasmodium vivax isolates.Florian NoulinCéline BorlonPeter van den EedeLuc BoelCatherine M VerfaillieUmberto D'AlessandroAnnette ErhartThe development of a system for the continuous culture of Plasmodium vivax in vitro would benefit from the use of reticulocytes derived from differentiated hematopoietic stem cells (HCS). At present, the need to use both fresh reticulocytes and fresh P. vivax isolates represents a major obstacle towards this goal, particularly for laboratories located in non-endemic countries. Here, we describe a new method for the cryopreservation of HSC-derived reticulocytes to be used for both P. falciparum and P. vivax invasion tests. Cryopreserved P. falciparum and P. vivax isolates could invade both fresh and cryopreserved HSC-derived reticulocytes with similar efficiency. This new technique allows the storage of HSC-derived reticulocytes which can be used for later invasion tests and represents an important step towards the establishment of a continuous P. vivax culture.http://europepmc.org/articles/PMC3402485?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Florian Noulin Céline Borlon Peter van den Eede Luc Boel Catherine M Verfaillie Umberto D'Alessandro Annette Erhart |
spellingShingle |
Florian Noulin Céline Borlon Peter van den Eede Luc Boel Catherine M Verfaillie Umberto D'Alessandro Annette Erhart Cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved Plasmodium vivax isolates. PLoS ONE |
author_facet |
Florian Noulin Céline Borlon Peter van den Eede Luc Boel Catherine M Verfaillie Umberto D'Alessandro Annette Erhart |
author_sort |
Florian Noulin |
title |
Cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved Plasmodium vivax isolates. |
title_short |
Cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved Plasmodium vivax isolates. |
title_full |
Cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved Plasmodium vivax isolates. |
title_fullStr |
Cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved Plasmodium vivax isolates. |
title_full_unstemmed |
Cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved Plasmodium vivax isolates. |
title_sort |
cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved plasmodium vivax isolates. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
The development of a system for the continuous culture of Plasmodium vivax in vitro would benefit from the use of reticulocytes derived from differentiated hematopoietic stem cells (HCS). At present, the need to use both fresh reticulocytes and fresh P. vivax isolates represents a major obstacle towards this goal, particularly for laboratories located in non-endemic countries. Here, we describe a new method for the cryopreservation of HSC-derived reticulocytes to be used for both P. falciparum and P. vivax invasion tests. Cryopreserved P. falciparum and P. vivax isolates could invade both fresh and cryopreserved HSC-derived reticulocytes with similar efficiency. This new technique allows the storage of HSC-derived reticulocytes which can be used for later invasion tests and represents an important step towards the establishment of a continuous P. vivax culture. |
url |
http://europepmc.org/articles/PMC3402485?pdf=render |
work_keys_str_mv |
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