DNA directed immobilization of horseradish peroxidase on phase- transitioned lysozyme modified TiO2 for efficient degradation of phenol in wastewater
In this work, TiO2 nano particle was used for two purposes: it played as a role of a solid carrier for immobilization of HRP, and meanwhile acted as a photo-catalyst itself. In this way, the loss of enzymatic activity during enzyme immobilization can be compensated by the coupling of TiO2 photo-cata...
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doaj-999bc4a7384f43cea62c002cf02fa71a2021-02-21T04:30:26ZengElsevierMaterials & Design0264-12752021-03-01201109463DNA directed immobilization of horseradish peroxidase on phase- transitioned lysozyme modified TiO2 for efficient degradation of phenol in wastewaterBaolin Chen0Xun Wang1Xia Gao2Jie Jiang3Mancheng Hu4Shuni Li5Quanguo Zhai6Yucheng Jiang7School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR ChinaSchool of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR ChinaShaanxi Key Laboratory of Comprehensive Utilization of Tailings Resources, Shangluo University, Shangluo 726000, PR ChinaSchool of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR ChinaSchool of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China; Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi'an 710062, PR ChinaSchool of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China; Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi'an 710062, PR ChinaSchool of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China; Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi'an 710062, PR ChinaSchool of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China; Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi'an 710062, PR China; Corresponding author at: School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, PR China.In this work, TiO2 nano particle was used for two purposes: it played as a role of a solid carrier for immobilization of HRP, and meanwhile acted as a photo-catalyst itself. In this way, the loss of enzymatic activity during enzyme immobilization can be compensated by the coupling of TiO2 photo-catalysis. Then, the surface of TiO2 nanoparticles were modified by phase-transitioned lysozyme (PTL) and connected with horseradish peroxidase (HRP) via a DNA helix (HRP-DNA-PTL@TiO2). The DNA double helix chain was utilized as a flexible spacer arm to keep the flexibility of the immobilized enzyme. The HRP-DNA-PTL@TiO2 showed a high recovered activity (90.52%) of the original activity of free HRP. This data is better than most reported activity recovery of immobilized enzyme. Moreover, HRP-DNA-PTL@TiO2 can maintain high catalytic activity, stability and reusability when degrading phenol in wastewater. The phenol of 0.5 mmol∙L−1 could be totally degraded within 10 min. Moreover, a visual colorimetric method for phenol concentration by HRP-DNA-PTL@TiO2 was established, and the phenol concentration of 0–0.2 mmol∙L−1 can be visually detected with naked eyes. Furthermore, an enzymatic-photocatalytic synergetic effect of HRP-DNA-PTL@TiO2 is found and investigated. The efficiency of HRP-DNA-PTL@TiO2 ensures it a potential application in the wastewater treatment.http://www.sciencedirect.com/science/article/pii/S0264127521000162Enzyme-TiO2 compositeModification of surfaceBiocatalysisSynergetic effect |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Baolin Chen Xun Wang Xia Gao Jie Jiang Mancheng Hu Shuni Li Quanguo Zhai Yucheng Jiang |
spellingShingle |
Baolin Chen Xun Wang Xia Gao Jie Jiang Mancheng Hu Shuni Li Quanguo Zhai Yucheng Jiang DNA directed immobilization of horseradish peroxidase on phase- transitioned lysozyme modified TiO2 for efficient degradation of phenol in wastewater Materials & Design Enzyme-TiO2 composite Modification of surface Biocatalysis Synergetic effect |
author_facet |
Baolin Chen Xun Wang Xia Gao Jie Jiang Mancheng Hu Shuni Li Quanguo Zhai Yucheng Jiang |
author_sort |
Baolin Chen |
title |
DNA directed immobilization of horseradish peroxidase on phase- transitioned lysozyme modified TiO2 for efficient degradation of phenol in wastewater |
title_short |
DNA directed immobilization of horseradish peroxidase on phase- transitioned lysozyme modified TiO2 for efficient degradation of phenol in wastewater |
title_full |
DNA directed immobilization of horseradish peroxidase on phase- transitioned lysozyme modified TiO2 for efficient degradation of phenol in wastewater |
title_fullStr |
DNA directed immobilization of horseradish peroxidase on phase- transitioned lysozyme modified TiO2 for efficient degradation of phenol in wastewater |
title_full_unstemmed |
DNA directed immobilization of horseradish peroxidase on phase- transitioned lysozyme modified TiO2 for efficient degradation of phenol in wastewater |
title_sort |
dna directed immobilization of horseradish peroxidase on phase- transitioned lysozyme modified tio2 for efficient degradation of phenol in wastewater |
publisher |
Elsevier |
series |
Materials & Design |
issn |
0264-1275 |
publishDate |
2021-03-01 |
description |
In this work, TiO2 nano particle was used for two purposes: it played as a role of a solid carrier for immobilization of HRP, and meanwhile acted as a photo-catalyst itself. In this way, the loss of enzymatic activity during enzyme immobilization can be compensated by the coupling of TiO2 photo-catalysis. Then, the surface of TiO2 nanoparticles were modified by phase-transitioned lysozyme (PTL) and connected with horseradish peroxidase (HRP) via a DNA helix (HRP-DNA-PTL@TiO2). The DNA double helix chain was utilized as a flexible spacer arm to keep the flexibility of the immobilized enzyme. The HRP-DNA-PTL@TiO2 showed a high recovered activity (90.52%) of the original activity of free HRP. This data is better than most reported activity recovery of immobilized enzyme. Moreover, HRP-DNA-PTL@TiO2 can maintain high catalytic activity, stability and reusability when degrading phenol in wastewater. The phenol of 0.5 mmol∙L−1 could be totally degraded within 10 min. Moreover, a visual colorimetric method for phenol concentration by HRP-DNA-PTL@TiO2 was established, and the phenol concentration of 0–0.2 mmol∙L−1 can be visually detected with naked eyes. Furthermore, an enzymatic-photocatalytic synergetic effect of HRP-DNA-PTL@TiO2 is found and investigated. The efficiency of HRP-DNA-PTL@TiO2 ensures it a potential application in the wastewater treatment. |
topic |
Enzyme-TiO2 composite Modification of surface Biocatalysis Synergetic effect |
url |
http://www.sciencedirect.com/science/article/pii/S0264127521000162 |
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