Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure
The aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical rele...
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doaj-99a2a9c308eb48029f881ee1a0fe785b2020-12-05T00:06:26ZengMDPI AGCells2073-44092020-12-0192611261110.3390/cells9122611Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological ProcedureJosef Vodicka0Martin Pesta1Vlastimil Kulda2Katerina Houfkova3Bohuslava Vankova4Jakub Sebek5Martin Skala6Jakub Fichtl7Kristyna Prochazkova8Ondrej Topolcan9Department of Surgery, Faculty Hospital Plzen, Faculty of Medicine in Pilsen, Charles University, alej Svobody 80, 304 60 Plzen, Czech RepublicDepartment of Biology, Faculty of Medicine in Pilsen, Charles University, alej Svobody 76, 323 00 Plzen, Czech RepublicDepartment of Medical Chemistry and Biochemistry, Faculty of Medicine in Pilsen, Charles University, Karlovarska 48, 301 66 Plzen, Czech RepublicDepartment of Biology, Faculty of Medicine in Pilsen, Charles University, alej Svobody 76, 323 00 Plzen, Czech RepublicDepartment of Pathology, Faculty Hospital Plzen, Faculty of Medicine in Pilsen, Charles University, Edvarda Benese 13, 305 99 Plzen, Czech RepublicDepartment of Surgery, Faculty Hospital Plzen, Faculty of Medicine in Pilsen, Charles University, alej Svobody 80, 304 60 Plzen, Czech RepublicDepartment of Surgery, Faculty Hospital Plzen, Faculty of Medicine in Pilsen, Charles University, alej Svobody 80, 304 60 Plzen, Czech RepublicDepartment of Surgery, Faculty Hospital Plzen, Faculty of Medicine in Pilsen, Charles University, alej Svobody 80, 304 60 Plzen, Czech RepublicDepartment of Surgery, Faculty Hospital Plzen, Faculty of Medicine in Pilsen, Charles University, alej Svobody 80, 304 60 Plzen, Czech RepublicDepartment of Immunochemistry Diagnostics, Faculty Hospital Plzen, Faculty of Medicine in Pilsen, Charles University, Edvarda Benese 13, 305 99 Plzen, Czech RepublicThe aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical relevance of the results was evaluated based on relation to prognosis; the disease-free interval (DFI) and overall survival (OS) were analyzed. During radical surgical treatment, a total of 1426 lymph nodes were obtained from 100 patients, creating 472 groups of nodes (4–5 groups per patient) and examined by H&E, IHC CK19 and OSNA. The median follow-up was 44 months. Concordant results on the lymph node status of the H&E, IHC CK19 and OSNA examinations were reported in 78% of patients. We recorded shorter OS in patients with positive results provided by both OSNA and H&E. The study demonstrated a higher percentage of detected micrometastases in lymph nodes by the OSNA method. However, the higher sensitivity of the OSNA, with the cut-off value 250 copies of mRNA of CK19/µL, resulted in a lower association of OSNA positivity with progress of the disease compared to H&E. Increasing the cut-off to 615 copies resulted in an increase in concordance between the OSNA and H&E, which means that the higher cut-off is more relevant in the case of lung tumors.https://www.mdpi.com/2073-4409/9/12/2611lung cancerlymph nodesH&ampEIHC CK19 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Josef Vodicka Martin Pesta Vlastimil Kulda Katerina Houfkova Bohuslava Vankova Jakub Sebek Martin Skala Jakub Fichtl Kristyna Prochazkova Ondrej Topolcan |
spellingShingle |
Josef Vodicka Martin Pesta Vlastimil Kulda Katerina Houfkova Bohuslava Vankova Jakub Sebek Martin Skala Jakub Fichtl Kristyna Prochazkova Ondrej Topolcan Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure Cells lung cancer lymph nodes H& amp E IHC CK19 |
author_facet |
Josef Vodicka Martin Pesta Vlastimil Kulda Katerina Houfkova Bohuslava Vankova Jakub Sebek Martin Skala Jakub Fichtl Kristyna Prochazkova Ondrej Topolcan |
author_sort |
Josef Vodicka |
title |
Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure |
title_short |
Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure |
title_full |
Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure |
title_fullStr |
Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure |
title_full_unstemmed |
Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure |
title_sort |
prognostic significance of lymph node examination by the osna method in lung cancer patients—comparison with the standard histopathological procedure |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2020-12-01 |
description |
The aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical relevance of the results was evaluated based on relation to prognosis; the disease-free interval (DFI) and overall survival (OS) were analyzed. During radical surgical treatment, a total of 1426 lymph nodes were obtained from 100 patients, creating 472 groups of nodes (4–5 groups per patient) and examined by H&E, IHC CK19 and OSNA. The median follow-up was 44 months. Concordant results on the lymph node status of the H&E, IHC CK19 and OSNA examinations were reported in 78% of patients. We recorded shorter OS in patients with positive results provided by both OSNA and H&E. The study demonstrated a higher percentage of detected micrometastases in lymph nodes by the OSNA method. However, the higher sensitivity of the OSNA, with the cut-off value 250 copies of mRNA of CK19/µL, resulted in a lower association of OSNA positivity with progress of the disease compared to H&E. Increasing the cut-off to 615 copies resulted in an increase in concordance between the OSNA and H&E, which means that the higher cut-off is more relevant in the case of lung tumors. |
topic |
lung cancer lymph nodes H& amp E IHC CK19 |
url |
https://www.mdpi.com/2073-4409/9/12/2611 |
work_keys_str_mv |
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