Combining metagenomics and metatranscriptomics to study human, animal and environmental resistomes
Antibiotic resistance (AR) is a grim and very common phenomenon around the world, posing a significant threat to public health and food safety. The emergence of multi-drug and even pan-resistant strains carrying novel antibiotic resistance genes (ARGs) and insufficient development of new antibiotics...
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doaj-99b8d6f0cec048be86ded438ab574eea2021-02-05T15:32:26ZengElsevierMedicine in Microecology2590-09782020-03-013100014Combining metagenomics and metatranscriptomics to study human, animal and environmental resistomesYanan Wang0Yongfei Hu1George Fu Gao2College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, 450046, China; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China; Corresponding author.CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China; Chinese Center for Disease Control and Prevention (China CDC), Beijing, 102206, ChinaAntibiotic resistance (AR) is a grim and very common phenomenon around the world, posing a significant threat to public health and food safety. The emergence of multi-drug and even pan-resistant strains carrying novel antibiotic resistance genes (ARGs) and insufficient development of new antibiotics are the major reasons that limit the choice of antibiotics for treating bacterial infections. Because of the heavy use of antibiotics in human medicine and animal husbandry, human and animal guts and farm environments now constitute reservoirs for antibiotic-resistant pathogens and ARGs. ARGs can circulate in humans, animals, and their associated environments in many ways, leading to a wide dissemination and spread of AR. Characterizing the collective ARGs (resistome) in host-associated and natural environments will deepen our understanding of the origin, evolution and transmission of ARGs. As many microbes cannot be cultivated in the laboratory, culture-independent strategies are urgent needed to explore the resistomes in living and nonliving environments. With the development of high-throughput sequencing technologies, metagenomics and metatranscriptomics are becoming important tools for characterizing microbiomes as well as resistomes. Here, we summarize the research progress and future directions for combining metagenomics and metatranscriptomics to study human, animal and environmental resistomes.http://www.sciencedirect.com/science/article/pii/S2590097820300112ARGsResistomeSequence-based metagenomicsFunctional metagenomicsMetatranscriptomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yanan Wang Yongfei Hu George Fu Gao |
spellingShingle |
Yanan Wang Yongfei Hu George Fu Gao Combining metagenomics and metatranscriptomics to study human, animal and environmental resistomes Medicine in Microecology ARGs Resistome Sequence-based metagenomics Functional metagenomics Metatranscriptomics |
author_facet |
Yanan Wang Yongfei Hu George Fu Gao |
author_sort |
Yanan Wang |
title |
Combining metagenomics and metatranscriptomics to study human, animal and environmental resistomes |
title_short |
Combining metagenomics and metatranscriptomics to study human, animal and environmental resistomes |
title_full |
Combining metagenomics and metatranscriptomics to study human, animal and environmental resistomes |
title_fullStr |
Combining metagenomics and metatranscriptomics to study human, animal and environmental resistomes |
title_full_unstemmed |
Combining metagenomics and metatranscriptomics to study human, animal and environmental resistomes |
title_sort |
combining metagenomics and metatranscriptomics to study human, animal and environmental resistomes |
publisher |
Elsevier |
series |
Medicine in Microecology |
issn |
2590-0978 |
publishDate |
2020-03-01 |
description |
Antibiotic resistance (AR) is a grim and very common phenomenon around the world, posing a significant threat to public health and food safety. The emergence of multi-drug and even pan-resistant strains carrying novel antibiotic resistance genes (ARGs) and insufficient development of new antibiotics are the major reasons that limit the choice of antibiotics for treating bacterial infections. Because of the heavy use of antibiotics in human medicine and animal husbandry, human and animal guts and farm environments now constitute reservoirs for antibiotic-resistant pathogens and ARGs. ARGs can circulate in humans, animals, and their associated environments in many ways, leading to a wide dissemination and spread of AR. Characterizing the collective ARGs (resistome) in host-associated and natural environments will deepen our understanding of the origin, evolution and transmission of ARGs. As many microbes cannot be cultivated in the laboratory, culture-independent strategies are urgent needed to explore the resistomes in living and nonliving environments. With the development of high-throughput sequencing technologies, metagenomics and metatranscriptomics are becoming important tools for characterizing microbiomes as well as resistomes. Here, we summarize the research progress and future directions for combining metagenomics and metatranscriptomics to study human, animal and environmental resistomes. |
topic |
ARGs Resistome Sequence-based metagenomics Functional metagenomics Metatranscriptomics |
url |
http://www.sciencedirect.com/science/article/pii/S2590097820300112 |
work_keys_str_mv |
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