Pharmacokinetics and Bioavailability of Carprofen in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Broodstock

• Main Authors: Kamil Uney, Duygu Durna Corum, Ertugrul Terzi, Orhan Corum
• Format: Article
• Language: English
• Published: MDPI AG 2021-06-01
• Series: Pharmaceutics
• Subjects: bioavailability; broodstock; carprofen; pharmacokinetics; rainbow trout
• Online Access: https://www.mdpi.com/1999-4923/13/7/990

The aim of this study was to determine the pharmacokinetics of carprofen following intravenous (IV), intramuscular (IM) and oral routes to rainbow trout (<i>Oncorhynchus mykiss</i>) broodstock at temperatures of 10 ± 1.5 °C. In this study, thirty-six healthy rainbow trout broodstock (body weight, 1.45 ± 0.30 kg) were used. The plasma concentrations of carprofen were determined using high-performance liquid chromatography and pharmacokinetic parameters were calculated using non-compartmental analysis. Carprofen was measured up to 192 h for IV route and 240 h for IM, and oral routes in plasma. The elimination half-life (t_1/2λz) was 30.66, 46.11, and 41.08 h for IV, IM and oral routes, respectively. Carprofen for the IV route showed the total clearance of 0.02 L/h/kg and volume of distribution at steady state of 0.60 L/kg. For IM and oral routes, the peak plasma concentration (C_max) was 3.96 and 2.52 μg/mL with the time to reach C_max of 2 and 4 h, respectively. The bioavailability was 121.89% for IM route and 78.66% for oral route. The favorable pharmacokinetic properties such as the good bioavailability and long t_1/2λz for IM and oral route of carprofen suggest the possibility of its effective use for the treatment of various conditions in broodstock.