Prediction of the mechanism of action of fusaricidin on Bacillus subtilis.
Long-term use of antibiotics has engendered a large number of resistant pathogens, which pose a serious threat to human health. Here, we investigated the mechanism of fusaricidin antibacterial activity toward Bacillus subtilis and characterized the pathways responsible for drug resistance. We found...
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2012-01-01
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doaj-99c4c45995b24f94ba284f9660f5c6c42020-11-25T01:13:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e5000310.1371/journal.pone.0050003Prediction of the mechanism of action of fusaricidin on Bacillus subtilis.Wen-Bang YuChun-Yun YinYing ZhouBang-Ce YeLong-term use of antibiotics has engendered a large number of resistant pathogens, which pose a serious threat to human health. Here, we investigated the mechanism of fusaricidin antibacterial activity toward Bacillus subtilis and characterized the pathways responsible for drug resistance. We found that σ(w), an extracytoplasmic function sigma factor, plays an important role in the resistance to fusaricidins during the initial 5 minutes of drug addition. Approximately 18 genes were induced more than 3-fold, of which 66.7% are known to be regulated by σ(w). Over the following 3 h, fusaricidins induced 194 genes more than three-fold, and most were associated with classes of antibiotic-responsive stimulons. Moreover, the fusaricidin treatment increased the catabolism of fatty and amino acids but strongly repressed glucose decomposition and gluconeogenesis. In summary, our data provide insight into the mechanism of fusaricidin activity, on which we based our suggested strategies for the development of novel antibiotic agents.http://europepmc.org/articles/PMC3503724?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wen-Bang Yu Chun-Yun Yin Ying Zhou Bang-Ce Ye |
spellingShingle |
Wen-Bang Yu Chun-Yun Yin Ying Zhou Bang-Ce Ye Prediction of the mechanism of action of fusaricidin on Bacillus subtilis. PLoS ONE |
author_facet |
Wen-Bang Yu Chun-Yun Yin Ying Zhou Bang-Ce Ye |
author_sort |
Wen-Bang Yu |
title |
Prediction of the mechanism of action of fusaricidin on Bacillus subtilis. |
title_short |
Prediction of the mechanism of action of fusaricidin on Bacillus subtilis. |
title_full |
Prediction of the mechanism of action of fusaricidin on Bacillus subtilis. |
title_fullStr |
Prediction of the mechanism of action of fusaricidin on Bacillus subtilis. |
title_full_unstemmed |
Prediction of the mechanism of action of fusaricidin on Bacillus subtilis. |
title_sort |
prediction of the mechanism of action of fusaricidin on bacillus subtilis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Long-term use of antibiotics has engendered a large number of resistant pathogens, which pose a serious threat to human health. Here, we investigated the mechanism of fusaricidin antibacterial activity toward Bacillus subtilis and characterized the pathways responsible for drug resistance. We found that σ(w), an extracytoplasmic function sigma factor, plays an important role in the resistance to fusaricidins during the initial 5 minutes of drug addition. Approximately 18 genes were induced more than 3-fold, of which 66.7% are known to be regulated by σ(w). Over the following 3 h, fusaricidins induced 194 genes more than three-fold, and most were associated with classes of antibiotic-responsive stimulons. Moreover, the fusaricidin treatment increased the catabolism of fatty and amino acids but strongly repressed glucose decomposition and gluconeogenesis. In summary, our data provide insight into the mechanism of fusaricidin activity, on which we based our suggested strategies for the development of novel antibiotic agents. |
url |
http://europepmc.org/articles/PMC3503724?pdf=render |
work_keys_str_mv |
AT wenbangyu predictionofthemechanismofactionoffusaricidinonbacillussubtilis AT chunyunyin predictionofthemechanismofactionoffusaricidinonbacillussubtilis AT yingzhou predictionofthemechanismofactionoffusaricidinonbacillussubtilis AT bangceye predictionofthemechanismofactionoffusaricidinonbacillussubtilis |
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1725161241837568000 |