Recent Advances in Monitoring Cell Behavior Using Cell-Based Impedance Spectroscopy
Cell-based impedance spectroscopy (CBI) is a powerful tool that uses the principles of electrochemical impedance spectroscopy (EIS) by measuring changes in electrical impedance relative to a voltage applied to a cell layer. CBI provides a promising platform for the detection of several properties of...
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Online Access: | https://www.mdpi.com/2072-666X/11/6/590 |
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doaj-99c7b9968bdf46b4964016de51dffbb92020-11-25T03:54:06ZengMDPI AGMicromachines2072-666X2020-06-011159059010.3390/mi11060590Recent Advances in Monitoring Cell Behavior Using Cell-Based Impedance SpectroscopyQusai Hassan0Soha Ahmadi1Kagan Kerman2Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, CanadaDepartment of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, CanadaDepartment of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, CanadaCell-based impedance spectroscopy (CBI) is a powerful tool that uses the principles of electrochemical impedance spectroscopy (EIS) by measuring changes in electrical impedance relative to a voltage applied to a cell layer. CBI provides a promising platform for the detection of several properties of cells including the adhesion, motility, proliferation, viability and metabolism of a cell culture. This review gives a brief overview of the theory, instrumentation, and detection principles of CBI. The recent applications of the technique are given in detail for research into cancer, neurodegenerative diseases, toxicology as well as its application to 2D and 3D in vitro cell cultures. CBI has been established as a biophysical marker to provide quantitative cellular information, which can readily be adapted for single-cell analysis to complement the existing biomarkers for clinical research on disease progression.https://www.mdpi.com/2072-666X/11/6/590cell-based impedancecell cultureelectrochemical impedance spectroscopyhigh-throughput screening |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qusai Hassan Soha Ahmadi Kagan Kerman |
spellingShingle |
Qusai Hassan Soha Ahmadi Kagan Kerman Recent Advances in Monitoring Cell Behavior Using Cell-Based Impedance Spectroscopy Micromachines cell-based impedance cell culture electrochemical impedance spectroscopy high-throughput screening |
author_facet |
Qusai Hassan Soha Ahmadi Kagan Kerman |
author_sort |
Qusai Hassan |
title |
Recent Advances in Monitoring Cell Behavior Using Cell-Based Impedance Spectroscopy |
title_short |
Recent Advances in Monitoring Cell Behavior Using Cell-Based Impedance Spectroscopy |
title_full |
Recent Advances in Monitoring Cell Behavior Using Cell-Based Impedance Spectroscopy |
title_fullStr |
Recent Advances in Monitoring Cell Behavior Using Cell-Based Impedance Spectroscopy |
title_full_unstemmed |
Recent Advances in Monitoring Cell Behavior Using Cell-Based Impedance Spectroscopy |
title_sort |
recent advances in monitoring cell behavior using cell-based impedance spectroscopy |
publisher |
MDPI AG |
series |
Micromachines |
issn |
2072-666X |
publishDate |
2020-06-01 |
description |
Cell-based impedance spectroscopy (CBI) is a powerful tool that uses the principles of electrochemical impedance spectroscopy (EIS) by measuring changes in electrical impedance relative to a voltage applied to a cell layer. CBI provides a promising platform for the detection of several properties of cells including the adhesion, motility, proliferation, viability and metabolism of a cell culture. This review gives a brief overview of the theory, instrumentation, and detection principles of CBI. The recent applications of the technique are given in detail for research into cancer, neurodegenerative diseases, toxicology as well as its application to 2D and 3D in vitro cell cultures. CBI has been established as a biophysical marker to provide quantitative cellular information, which can readily be adapted for single-cell analysis to complement the existing biomarkers for clinical research on disease progression. |
topic |
cell-based impedance cell culture electrochemical impedance spectroscopy high-throughput screening |
url |
https://www.mdpi.com/2072-666X/11/6/590 |
work_keys_str_mv |
AT qusaihassan recentadvancesinmonitoringcellbehaviorusingcellbasedimpedancespectroscopy AT sohaahmadi recentadvancesinmonitoringcellbehaviorusingcellbasedimpedancespectroscopy AT kagankerman recentadvancesinmonitoringcellbehaviorusingcellbasedimpedancespectroscopy |
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